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Z Z Z Q R R s s s %%%0/4::CEENOOXXXaaajjjsss|{{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9656e95fce3e47bf9c4ef8341c75d379Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-04-13_22-08_0517 3130Seq 12232 12232_Costion_K1355_matK_R jmadera 96-Well K1355_D3KB_3730_POP7_BDTv3.mobSeqPop7z !%(+-02431.)&$%(,-* u e _ _ b d b ] T J B ; 7 5 5 3 1 , & " $ " ! 0 D Y l y +=N\is|iN1 { t k _ Q B 4 ) # " & , 1 2 0 )
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& " E ' " 4 K ! 6 W & L - ! Q ) ; # P + S " A E K " " 2 . h ! , = / 0 $ V . H o , C C Y J T \ , $ C ! # ! > $ = 2 O ) 5 # % & . , ! N $ ' m r ) ) P A * " ! a 8 < D 5 T 3 2 1 # $ 7 + . " 0 ( / ! $ ( $ " " Q \ z # @ ! : # - M " & % C ` $ U 8 Z : Q 6 F X < ] 7 . / & < > # ! . ' . 3 ' 0 + a % " 1 i m J + O / = & ' K 9 $ 2 5 % > / g 0 $ ! I 3 d ' 8 < / i 3 Z 4 " 0 " & = $ ! = < : D 8 3 2 1 ( $ ? , - - % 7 . f 9 A 0 ( * / 3 $ / ; 8 @ + ! B $ ! - , K^ 3 ( # 6| = P Y t A , < 3 " + * 3 5 < X 1 " , & N ! [ 0 \ . > ? q Q l h g [ B + 3 M Y O v G = d : & F , J O : ? Y 4 C $ T P b - K " w M _ - T O Q 5 # 6 A _ # 6 ? k * # " 0 ? ? 1 > ^ D , ) 0 ` $ 5 B - O B ^ / i 3 . c * 2 = = \ z L 9 ' | ? S ( 1 ( @ ^ _ N E H R _ E W S ; 7 ; ! $ Q } @ q + M j l k K 7 M U ] , ( / - . & d E p J ] 8 8 6 C 9 y R w + ' z 3 [ 8 L! A r d 3 ` N h 9 w O C R _ B 7 T > ` o 8 p * v [ ] A X : P ( " H K s 6 b^ K3 5 D h J . 2 RL o f j ,f p T - 3 U +n = Q$ t X )N 4 BW "L 0 7} 6 ?AAGCGTAATTAACTCAAGGAATGAGATTATACGCAATTATTAAGAGGGATACCTCCACCTACACGCAGGTACCCGGCCATTATAACAGGAGCCGAAGGATCCGTACCAGGCCGCCTTACCGAAAGGCGGTCCTCCCAGGTACCGGACCTCGCCCTAGACGACGGATCCATCAGTAGGCTGAGCGGGACTCGTGGCTCGAGCAACAGCATAGCAGTGTCGAGGAGGGGTGCAAGGTCGGTGGGGTGACTTCCCATTACTGGAGGTGTTAATAACACTCCTGGGAGATCATTCCTCGAGTCGAGAGACGGCATCGAGCAATGACAGAAAGCGATTCCCTCCCGGTGAGGGCACGCAGGAGGACGGCAGTGTCCCGTATGGGGGGGATGAGGATTTCCCCTACTTATGAGGAGAAGCGGGATACCTTAAGCCATGAATTGCCCTTCCTGATATCTGGCACTAAGGCTGCGAAGATCCGTGGGGGGGGTCCAGAAGTCCCCCCACTCCACAAGGATGGGCGCCGACAGGAGAAACAATAAGGCTATGGGGCTATATGCGCCCACGGGGGAGTATACAGGTTACCTAGGACATTTGCGGCGATAGTTAACTCGCAGGGTTCGGAGATAGACTTCTACTCTCCTCTCTGCGTACTATAGAAGGACGAGGAGTATCGTATAGTTGCCGGAGGGGCCGAACTACTCCGTAGGAGTATTCATCTACTGCTAACACTGGTCTCATAGAGGGAGGCACGTGAGATGCGCTGAGGAGACGCATTCCTCCTTCAATCGCATAATGCGTGCAACACTATCTCTAGATAGATGATATAATATAACTATACAGGTCACACCTTATCGTTGTAACACTACGTGTCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-04-13 23:39:46 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt y ASPt y B1Pt x B1Pt x BCTS n BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ( Q DATA
( Q 6 DATA ( Q DATA ( Q ڹ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ mZ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS , P1AM d N P1RL d H P1WD d U P2AM d c. P2BA d d i P2RL d \f PBAS d d 0R PBAS d d , PCON d d D PCON d d AF PDMF PDMF , PLOC d :~ PLOC d 3 PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 3 RUNT '
RUNT , RUNT % Rate RunN " " S/N% , SCAN 3 SMED
SMLt SMPL SPAC Abb SPAC , SPAC Abb SVER 4.0 SVER n SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH , phDY big phQL c phTR phTR \ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt y ASPt y B1Pt x B1Pt x BCTS n BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ( Q DATA
( Q 6 DATA ( Q DATA ( Q ڹ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab }> FVoc }- FWO_ GATC Feat / / | GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ mZ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS , P1AM d N P1RL d H P1WD d U P2AM d c. P2BA d d i P2RL d \f PBAS d d 0R PBAS d d , PCON d d D PCON d d AF PDMF PDMF , PLOC d :~ PLOC d 3 PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 3 RUNT '
RUNT , RUNT % Rate RunN " " S/N% , SCAN 3 SMED
SMLt SMPL SPAC Abb SPAC , SPAC Abb SVER 4.0 SVER n SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH , phDY big phQL c phTR phTR V"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'8AgtuO#H~J$mX%r:M\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvU)h'~f+ ]Vv65MT3 #pJE?RVuU,\)lOYW|_nw|]jL@A[]"dMI[h?&0E(_y"X_tXL ViY2]2rRU)>`VZI;P>W+YYo%+04NRY0GbR31}ssb[l"G>}O#pJl&v`VfXr9lKd,}Sn2M5FiKKB*oCaRZ^.EAx8aPI^QJH1}1"/]E]jWJDzbVFJ=`mcTv7KO]q6Po\s!&&v_QjXaq6.>`A?qm#vRRC(vJv:cGavedMNz/b_bRohj4TIx*&`OE!0z^n7aC<)\iyKG>?hECel7B]%j'k0$9eKoQD5pYHT+(aQQxnJilXSXW .C=vz^`%C]Cp38VazgHf9K:q++sbp%H+r,]_03BSmv^N6T.iP2i#G2rfJX|7"Wa%#;-T)RJ0vrs|%3WT?@\xoF5K.Ijd!FwMz&4-@6|k.j,PCH-p}*4d#ky`[qDr_WG\|xT;#6RlSFy!{]-j5OPh~wA[h@t?"\=QtAcP@~~JBv+c8*+C!K}Z'BNp3<(+(aQQxnJilXS_d.u?zcw~CfF{*Tijh.iOW/D-]$/pTFT*u;S AEPt 3 AEPt 3 APFN APXV 2 APrN APrV 1 APrX } } ARTN ASPF ASPt y ASPt y AUDT ` ` Q B1Pt x B1Pt x BCTS n BufT ' N T CTID # CTNM ) CTOw D CTTL L CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ' N ^ DATA ' N DATA ' N DATA ' N H DATA ( Q DATA
( Q 6 DATA ( Q DATA ( Q ڹ DCHT DSam DySN - Dye# DyeN 8 DyeN = DyeN B DyeN G DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab }> FVoc }- FWO_ GATC Feat / / | GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ mZ LIMS ! ! U LNTD 2 LsrP a MCHN MODF v MODL 3730 NAVG NLNE ` NOIS , P1AM d N P1RL d H P1WD d U P2AM d c. P2BA d d i P2RL d \f PBAS d d 0R PBAS d d , PCON d d D PCON d d AF PDMF PDMF , PLOC d :~ PLOC d 3 PSZE ` PTYP L PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 3 RUNT '
RUNT , RUNT % Rate RunN " " S/N% , SCAN 3 SMED
SMLt SMPL SPAC Abb SPAC , SPAC Abb SVER 4.0 SVER n SVER , , Scal @ Scan 3 TUBE D3 Tmpr < User phAR phCH , phDY big phQL c phTR phTR