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"  ",2Jn{`2&      &!3:Dg{)<xmD6 &=8tS{~]D28 " ">  <Vugh>.3#    9<?s#*vYB&      &!P\}X6'"    /6g{saroqJ.!&NLkFE9;F1<9.0.%/-4]i|yM8!(   29]qP:  +Vi{F>-0MO;   7LrFa%$ 3NWr\H"%5fwlM& '  !?J[\uRG3  +antO7!  ,H\cphGY>=  "5"/^em[<%%SZtw|`D;7#   ,?UZ~}~xHF!4$?JI&#! 5;XCF=-'+0AiXbYhF03 '194LSNNN)#"16H^fywMMD-!,E9_WHEG)'8*9% ":;8<., *)+!+  0K\_\EG; %9E@?5#61<=DH'##$ 87-[GbMTSG  $@(29 #$ "'+'' &  -9&38(% ..-""  .C56-" !3)7*'58345.,$#3+JC<0,0 %9(  AE80A9%  -!2/17'% ,K09B-  *$"/# ,14353A,"  +  " $+#$<  /5$! $8,-*,"      #)3(%+ ($&+ #' 2/-.79>8%!5*'/C<1#(/ %!,):(  &*  +(        !#  $    -  #    +   "                      !     %        $      d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRA  ZZZQRRsss%%%0/4::CEENOOXXXaaajjjsss|{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e484de652aa6495f9728ec700711cd67Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-04-13_22-08_05173130Seq1223212232_Costion_K1355_matK_Rjmadera96-WellK1355_C1KB_3730_POP7_BDTv3.mobSeqPop7z  "!%/6972-*,2<IU_gkmnmnpv~  ypf[QHB?>=93+"%),-*'$#$'))& %)*'" #$# "#"  !$'*09FUfvrbTF8)      .WC=)MVI,pmuuAs0nc`afp~.Ll3`wsSS}Q- 1EWdjeYG3!  !1F^xmO5! %H{ \)FJ5 CzK*/`W2C1Zf4+VDiyrW,Q+,=KXbiljcUA+ !=[vy_B' EA1]I^{9   $K{!+$ O( $N1A0E     (QOxrA]!YMPl5  "!    /GflD#     .^daN !*.*" &++& )=NVTH5"/R{oC 'KwY5+6=>:2(  ":Um|T7-;`,5!v?     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A]JQ2_<5/(V2332T$_+N`<2( D3V(,*<2(/S522312Va!EO1TPT2V&V22|  h8vllf`PzNpe|^de9r@ ~qO-X?MSQ6Re)#S Jr9m&qD oS- eS.[eGg,G/pARctO(bnx!` (oM6%v?Lqt'<w{oqV!_,}Z2dDv>{ ,+<42sQBV3e8v!(2&B)j mnN^Q1Y8IIph%wO' >n` w*$mzEMX*> R`~A)PUKC *RM\if54az\Rk I{5y]0a   EazKw-+a=-|& &#W\4=X~i^sh qnH+dH]/3T/ Uf#l>HTJ"S5): a r2Q7 w-d-)e.X!).y "k+\h'OSL_8EQ}7c*Nu,|7|fJO #7^p]BaC8[gW1RV\6eGr7b R  I,@iJr4TemD=/W7 o6> (,rr)M7jpd/{. &i><'+N8</ FVU31#" *3&%Y 5!     c5 2 9,."&,(  #3 # ~&&  7(61>["/ " 3*<& &)90( *N&@D $ %TSC//2 +0!I 22=  ?,5 2!9999$$$#5E$$<5CA$$W$$$A! ZZ9N&DLPPvPPPPPPZF/'T99gJQgpO``_$L DDDD2QXXD0DDDS ``H{j0zz?I`VDDDDk;jjKZNMMMM3^^_SfP^'QM}V9x[6_J;cc dcvK cccccPccHcMMMMMMMMMMzz_zzzM'~MMUMMMMMMzzziYYYYYYYYYY|hhhhhhhhhhhhhhhhhhhhhhhppPpIH1 /pi ;+'L,M8X_I<KW7=~  Zj,lPsJ!! dh * 4~78 5K1X#/EPDQ=N>0SW-LhpJ68C4xzfN,t]ZN,8 7@Y R?V'.SX^,,Hp*S,N|p\6o[pEUp,QM+hP|:DV@X,@^|qi\#g. 7 ,lC&K`B P&$pf{p^^f 9u{pE7\6wQ,"pbd,.) <"tsL7x^cppppp<:gg%_,JlxppObW-M  ^o>2YTo14C^&"a0&!Zk\2#:{'/,MlV)W$,XAK)@'5A^rtHh,px]UZ.T}<k1GUz dGksz.V3I.";X+%2m)0;'&pA/0s*+)fS(q9d/(*'A30[R=8`WuH*p'$.=2g193D4z#-X/*meJva<Y2-%/1E*Qhr;=V/2V@4=d(>G3K.P8Ph,G=A3+AaID4HXeuD&)D=?\Jb&51;@$U)CBN!(UL=!Fm4Gq&K* /+>]Cs_j.);A8+7,K,%@l{:@%g;c\)Uc7| >KQa-bx `"L5e\r{s&>4p!Q]JW5Xo@[g2|Z{:t&bZR->?q['3reW [TR_ia(kLRn0Qeb>W~f]+7$g 1R=h`s1tv/Bjo $O^u*0}|lO^w:0>,"P4nMs$QV8V\3V>t<i  ff\]h_brsEhQx M{sQ(!){%tFMt^N7c`s!Qi"6 V~wW:Z9bN)wDArM^ATTTATTTCCCACTGGCGCGGGTAGATATACGAGGTTATTTACCTTTAGGCCGCAACCTACTCACCCGGAAAAGACCATTATCCGAGTCGCAGGGGTAGGCAAAAGCCGATTCACTAGGTATATGATGTTGCGGTAGACACTGTGGGAATCGATTAACGGATTCTCTATGAGAAGTAGCCGAAGGGTCTGTCACGTCGGAAACACGACAGGGCTCTTGGAGAGGTGTCTACCCTAGCCGGGGAGGCTGTAGACCCCAGGAAAACTTAGGCTCACTAAAGGGATCCCGTTCCGGTGGTTGAAAGGGAACAAAGACTGGACGAACATGGGGCCACATCGCTTGCGGGGTCATGGTCCGTGCTAACCCCATGGGGTAGAGGGGAAAGGTTACCTTCGGCACCTCGCGGGGAGGGGCGTATCCAATGGAGGCATTCTTTATAGCCCCTGAGTTCCGACACCATGTGAGCTAAGATTCAAGGGGAGGATCCAAACCGATGCGGGAACAGGGAAAAGGACGCCGTCAGAGTGGTCGAGGTCTCCGAGGAGGGAGTGCCCCCCGCGAACGACCCCTAAAACTGTATACGGAGATCAGCTGATTTGTTACCAAAGATCTAGCTACAGGCTCCCCACTCCCACACTCGTGCGTTCGTAGGGTCACAGTATCGTCATGCTCCCGTACCCGAGAACAGGAGGGATAGTTCGGTCGGTGGCAAGGCGTGTCACTACTGCCCCTTGAGGATTGAAGAGGGAACACGCATAATTATGAGAAGACCTTTTCTCCAACATTCTATAATAGAACACATAGATACAGAACGGCATGTGGGCATCCGAGATGCCACAACABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-04-13 23:39:33 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSezBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (P DATA (P 4DATA (P  DATA (P +DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~cLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS%SP1AMIF`P1RLI?P1WDILP2AMIZP2BAII`P2RLISPBASII(PBASII%PCONII<PCONII9<PDMFPDMF%jPLOCI2PLOCI,PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 3RUNT ' RUNT ,RUNT %Rate RunN""S/N%%KSCAN3SMED SMLtSMPL SPACAf SPAC%cSPACAf SVER4.0SVER eoSVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH%phDYbigphQLcphTRphTRGThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTSezBufT'NTCTID#CTNM)CTOwDCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA (P DATA (P 4DATA (P  DATA (P +DCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabsFVocsFWO_GATCFeat//sGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~cLIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS%SP1AMIF`P1RLI?P1WDILP2AMIZP2BAII`P2RLISPBASII(PBASII%PCONII<PCONII9<PDMFPDMF%jPLOCI2PLOCI,PSZE`PTYPLPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND  RUND  RUND  RUND  RUNT 3RUNT ' RUNT ,RUNT %Rate RunN""S/N%%KSCAN3SMED SMLtSMPL SPACAf SPAC%cSPACAf SVER4.0SVER eoSVER,,Scal@Scan3TUBEC1Tmpr<UserphARphCH%phDYbigphQLcphTRphTRom;PsqD557%6C}:*SM|aZA /GAjrS$I),_A#$O7{_O[T6S$gI{cTKcaza(RO[mOEW<#GnP Qc$@)kOie&>2 xX|L=6XO?Tve!@;wW14^M1}Gu9v_QjXa*`)l@.u!n`%Sz*1fCy:*0avFwn&K2fE[p!CODa)x+7E2]Lv3?%diR)6/lA^5ehJb@4}%P!SNmWT/OKOvx4G!?`U!9@mwyy>T3pb}045@u:r*nOrozc{G[ZI,|Q|T{yK{1P3;fTR[fRr;-YVJ|Q:{+ z9X=5>2jDTWy2>y^q3Th$Y thENWNv3[My J1\QWi.HhrjcngzE<=#d1a-cF~bvHN+MzL6L!<7J8_M4;-6`Zdg~P_F7a;A{%ipoWeV&jJDeVE+hj3EYWC.%obB`!3xrM "K_l+VjWfz|luN+l4M,A 0wz`&1ZG|JW/e2FVc2(_CSR{[}7"=Iw<1C{_@6tKR"\pI2r>6Z~=}F)h>xgY5"KBv'o>s1HT&}J39OQJMeP> A(_GZE?RVuU,ZBE=+^k42g|5[S*.JXs2M*;N/VMR{7gmUo:e.s$;_CP)DzEC>3f9bb<4z@=[^GHF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|$q_{U3Nb04Z,JUtG_#4~Vu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_VL-&]S9Hgn_RDO6',>71B!Hiwe2{sGitG