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m s [ _ Z A e 8 J 8 = ! 6 , P B : 1 0 , < ? ? = 7 , ( % ; J N 9 1 7 8 3 2 X F P : : D B @ c F S b N i b } p s .KY|~wU[MU4 v } ~ d h W V O ; E G S \ ` l W Y X X g q d n (4e,8n,\hcsnNR hI NF3 l z i d c f e m S _ e U T l O S n e k } #*Y @^icjnU8|O? n m b g W R = \ O V T ; g N Z G X L ^ J Y J = F S R ] O c f b } { c | .Z_#OOp_oNM1(W; s q p T [ B V V Z S R K N X ^ [ d q a a ] g u { q 'A^q13PfaftUA(y]]\FQ1*=I1EDP_q[B. y o Z j m [ K M ` 9 H F O V J I @ s l k \ r z { b k i ] [ b T Q M G X \ C : J 4 = 5 X = A L 7 2 L E B 9 - 9 B 2 7 K : 7 / C < : J H 7 * , 4 - 5 A / 6 ; / / & 5 D G 3 2 6 ( 6 = @ N 1 F I G / 1 3 > 8 6 * 5 5 - * = - Q Y D a N M a g s u p p 2JZ^C 3 | s d | e _ f } k n { z &*DEIq}mwvubu|SWM=4# p y m j d T ] E ; 8 C P H P ? 7 9 N E B F J F > H 2 B 6 B 2 0 @ 5 ; I : / @ = C H A V Z a V a Z \ r 2WYnrG+ u h | u u n ~ )$1GY^xXsN|sqTk4=!9 2 x d { k j b d s g \ X h m p c e p j v GRfi~tG/ GPgV K; `BB0
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| j s [ p _ s S v Y a [ h k [ l _ n V Q c S f n y x !1{|qljD4 y z x q u t { | p z y t h z p a T a c R m c j o v y | t ] l ^ ] ] C U S U D A K N Y J Q R Y I A A C F @ G R = K 6 K C K Q \ : < 5 E Q P M ^ \ j ` t y d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 4AKPRSSTTTSRRRQPPNMLLJIHGEDBA@>>=;:8 K K L D D D _ _ _ u u u (''000777>>>EEELLLSSRYYY`__fffllkrruxw|}} < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f579d4a1d30b4a9798e07b208a9397d0Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////
////////////////////////////////////////////////////////////////////////////
//////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB
AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA
AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA
AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA
AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-09-04_11-32_0269 3130Seq 12982 12982_Steier_PueblaGap4-5_matkR jmadera 96-Well K1361.B2_matkRKB_3730_POP7_BDTv3.mobSeqPop7z i h i l s ~ -1+ $Hs ?Zn
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KB 1.4.1.82018-09-04 13:04:00 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt } ASPt } B1Pt | B1Pt | BCTS \M BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA '- NZ DATA
'- NZ 2 DATA '- NZ X DATA '- NZ ϲ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ Z LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM / ^ > P1RL / ^ 7 P1WD / ^ D{ P2AM / ^ Q7 P2BA / / W P2RL / ^ J PBAS / / !v PBAS / / G PCON / / 4 PCON / / 1a PDMF PDMF + PLOC / ^ + PLOC / ^ $ PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
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0 RUNT 6. RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL u SPAC Ao1 SPAC $ SPAC Ao1 SVER 4.0 SVER \B SVER , , n Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH B phDY big phQL c phTR phTR MKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@Bg-F!'8-gtwzqH\4K>G#Kz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAlI{_*.9nWFa-st2hSXdF#lQ%cy-*7y 4/a"XLGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#xg>33.BkST{!Xf] MzQd)l'[)VBe$O!&HoCdNmbcnPn&p;s_|g+XfT;KB{s2fo;LY&03t0mP`k:!.tkj^4E2*pJ!gxT6xh!DWnJ@5O`575H1FA:9Ic@OQ(x[8NhIx5KX9m3*{MBfK3tbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^i AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt } ASPt } AUDT l l x B1Pt | B1Pt | BCTS \M BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA '- NZ DATA
'- NZ 2 DATA '- NZ X DATA '- NZ ϲ DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab j FVoc j FWO_ GATC Feat / / jh GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 6 LAST ~ ~ Z LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM / ^ > P1RL / ^ 7 P1WD / ^ D{ P2AM / ^ Q7 P2BA / / W P2RL / ^ J PBAS / / !v PBAS / / G PCON / / 4 PCON / / 1a PDMF PDMF + PLOC / ^ + PLOC / ^ $ PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT
0 RUNT 6. RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL u SPAC Ao1 SPAC $ SPAC Ao1 SVER 4.0 SVER \B SVER , , n Scal @ Scan 3 TUBE F8 Tmpr < User phAR phCH B phDY big phQL c phTR phTR