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@D g r g { T [ \ \ _ F H I Q Z D V R S F G U [ M X D Q Y Y C E ` J Y D H E I Q N E \ G c Z Z Z l s t ,S/ --*9." uR e e k i f ` S Q > 6 Z C A O P L K G D 9 @ 9 U W C E J U K 2 J ? > F N C H P Z a L M Y Q \ l k } | y p y y j _ d Y g c _ X g ` q r 4QugqL;) | u o w j i b a O X X O \ I F M W I E I T ; ; T M J D M l d { t n o Q b U T L R S l X ] ~ j s g x d S Y U N [ X E V U E U j \ s a N ^ _ X N A C > P D A F M ? 1 B E ( B S G 0 F ? = F H E > < > J @ 1 J A L : < R J Q g b u u z p ] Y L K Q 6 ? > A B = 6 2 L 6 / : 1 @ ( @ E D - I @ / E 9 1 ? / G = N < J ; K C 6 N h c [ ] W J A @ = > 7 3 ; 1 4 E C M F F ? T E T L L Z b U ] Q W l l z k e m k n f k b J m N 0 J > H < > = : ? 4 3 I ? 2 C , < 4 : 7 6 ? 5 ? 9 R X ` q p j d t l [ P K S T M V L D P 9 U \ D K A > T 8 B D E > Q _ d r z f [ ` P Y _ D 9 7 A H = 6 : + 5 B 4 5 < ) I D > I 0 J B ? 9 7 H 3 H 9 # 9 1 : C I 7 B F < 7 P < - U O ; B ? N D M N P S F c = h [ P f h \ ` K c k n n } DOprno|}\M>)./:@{A. r ^ n s _ j Z S ] V N m I C K N H M : > V @ C 2 9 5 C : @ Y B 6 : L \ P P k w DMmxsX`R { { y h b c L Y J N G : < ? W 1 > 8 8 H Q 6 E 8 ) C A B ? O = L G ^ L j } { e j Y b P P > Y P S H D F E @ = L ? < ? < = 2 @ ? I ? 2 : @ : < G 6 * 6 7 ; < 1 D 7 4 8 @ F C P 7 H K > : A J D = 9 6 8 8 = ; F ; A 8 K T G B J F [ \ [ d ^ l IYGpbqQP&P8 F8Tica|fTK% p ] ` ^ h t (5Dj_G5 x | o x Z X o e v v | r v { ~ x e d a S Q 4 < : 5 6 B > + ? 5 % % . 2 1 ) 0 , A : 2 I C A - 8 2 7 = = * ! < 9 6 > 5 / 9 & ) / ) / / 1 2 2 C E ? 3 > 6 6 = 8 , : / 1 $ 4 + 6 3 F 1 0 8 1 4 # ; 9 ' 5 / 4 ! 5 2 / / ' 6 1 ) 6 C @ , - ' 6 @ < : 6 7 2 1 ? + 2 3 4 % A 0 * . > F > % & , , . 7 0 ( # , $ - # $ , / 3 0 1 . 6 " ) % ) ! & 3 6 , . / ) / 2 > % 0 % 5 4 > 5 / 7 . ' 0 / 6 . G C ? ; C , ' 3 1 ) @ , 6 ) A 5 3 0 8 / 6 9 0 3 / : * ; 9 < < ; * F + 5 7 < 4 = < 7 = J = V a j g T|xd;K9#.3J*2! m f v r j c N f @ T R A U C K 0 > < ) ( - 7 - 2 1 < . 1 - 7 @ : 4 8 . * , G 4 > & / ; @ @ Z I k c v x x n Y H L A K ? ? 0 8 3 : 2 ) 2 3 E 0 + + < ; + 0 5 6 0 ! ! ( 8 - + 3 3 ; E 5 7 % @ K P Q Q L ] j x g x p f T : P L R A N H ? 2 > D L 3 = I L I f M h b f s i { s m f } \ d O H 4 I E N / 6 E D / / 2 < ; 9 3 3 = 2 # = * $ $ 6 0 - 0 7 9 2 " 1 " 2 / / 0 8 5 * ; & ( ; , 8 # 4 & 5 ? 0 L 4 R R X p s z | o d h K [ Q F J M = . 1 ; 4 2 % & A : , @ 9 ) ' 3 ? C , 1 B 1 0 @ @ H ) / 1 < H . 3 G 8 I G D X n k ET`grtz`vkwt}`0 ] i W W ` Y E G K G A = 6 ) ; . 9 9 4 B 2 : < X 9 H A : ; 8 C E \ 7 ; ; : K > W J A E C < 5 @ , , H 7 L D Q J K p z x v p X Y Q I : K [ 4 9 ? ) G H C F B < G O Q V = K d [ h g $F`v|\OM3 u e ^ ` P W [ < L N ` _ x r j U R L V H E 6 H E . C < < + ; 8 ; ; 1 % 5 3 1 = 6 * C % , , % 3 4 # / ) , . 7 ' 3 , 6 : 6 0 6 1 6 - ( ! ) + 3 + ) & - 2 1 ? - & 0 0 / % 0 $ $ - ! 0 % 1 & 2 - 3 . - 4 0 " * $ 5 0 4 ' ; : 2 , F 9 ; - 2 E O . + H O N U r o
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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 625795d5faac44cda0ae6a154ae96301Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-09-04_11-32_0269 3130Seq 12982 12982_Steier_PueblaGap4-5_matkR jmadera 96-Well K1320.C10_matkRKB_3730_POP7_BDTv3.mobSeqPop7z p o m h a W O M R ] j y u h ^ X Y _ j x &Jg}#?]z %/:A@6% mX> wl\G,
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J CAAAATAATATTTTATCAGGAATCAAACCTATACAATAATAGTATGAGTTTACAAAACCCTATACAGACCTCCTCGCTATCATGGTTCTAGCCCTTCGCTATGGGCTTAAAGACGCTTCCACTTTGCGTTTCTAAAAAATATTTTCCCCTGAGAGTTACGATTGGGGCAAGCTTTATACCACCAGTTTCAATGCAAGCCGGCTCATCATTTTCATAACTAATTGGTAAACAAGCGGGCTTCCAATAACTTCCTACGGATGTCAAAATCAATTTTGGCGCCCATTATCTAGGTGCCCAATCTTCTCACTAACATACTAATCTGCCAGAGGCCTTCAAGGAACGATATATTTTCTAAGTGTAATAGGAGATTCTGGCAAGAGTCTGTGCCAGGATTACACAAGCGACTTATGGGTGTTTCAAGATCTTATCCTGTCTAGGGTTAGTTTAACAGCACTGAATGATAGCTTATAAAAGGAAGTTTTTTAGGAGAATGGGTTGGAATATTTCCTTCGTAATTCCACGAATTTCCAATTTATGCTGAGGTCCGAACACGATGAAATCCAGATGACATGATTATCCTTTCTTCCGCTAGATGATATAGATTTACATTGAGGTATACGGCTAAGCGCCTTTATGAGGCGTAGAGCAGACGCAGGAGACGCCAATACATAGGAAAGAGATATGAGCATATTCTAAATCCCAGGCCCTATTAGGCGTCTACTTATACAACACTCCTGTCTGTTATTGTCTCGATGTGCGCCTCCCGGCGTCTCAGCATTCGGGCGAGAGCGCCAGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-09-04 13:03:14 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Ox BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA % Kz DATA
% Kz 0 DATA % Kz { DATA % Kz DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ M LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM : 1 P1RL : + P1WD : 8$ P2AM : D P2BA J P2RL : >^ PBAS PBAS PCON ( PCON %v PDMF PDMF PLOC : < PLOC : PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT
0 RUNT 6. RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL u SPAC ArO SPAC SPAC ArO SVER 4.0 SVER Om SVER , , n Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR -i This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Ox BufT " D 1 CTID CTNM CTOw ! CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA % Kz DATA
% Kz 0 DATA % Kz { DATA % Kz DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab ] FVoc ] FWO_ GATC Feat / / ] GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ M LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM : 1 P1RL : + P1WD : 8$ P2AM : D P2BA J P2RL : >^ PBAS PBAS PCON ( PCON %v PDMF PDMF PLOC : < PLOC : PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT
0 RUNT 6. RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL u SPAC ArO SPAC SPAC ArO SVER 4.0 SVER Om SVER , , n Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR jk:/R",fq;,`h?X??-|D>jH}3^ %_ef2rgHGygLVFX^smgnWuYvQm{p,nhT}{Uw2yy>T3pb}0Zz>z5Cw1ubkv:cGave>u\nLTH:#6=ANp[F5TY/5`agv9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&xrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>RbS4}(Qbq`>?0g]^GnCz:"]ddc}"Qz)l]iOVe`A #pJEa#;{U,HB*@Yy-4$&+VFl@i2+`'Mvf0ido!v#`hDdAfor.H1+`AmHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob{\`d\}5L6Q^Zew.k.d}uNf1&vM\#YTEl?&azz,NZ9bY*R2'gFd+({y(9M%_|_n?7.N{C]2ZE<}DU$~~8WYH|O[nYI.xv^$be1Cd}F~8@>G>]l"G>}O!p8NpdUuk} 9`^avedsC\#`t3O[cKP8)s5AVih?]|_nTJL:SVX*oE}:36m~2HBIkij7!}WQ`Q&]t[XvXVou:fDqd*hIerElyYD8JURa$NwBx6Ks;6R&8yT,I)YVJ|Q}.z zF;#G)jp~4s*MPH}& d+[Y5xo|[Ez1{zK90lVS8G$l=b d[qm#vRRC(vJnPk&?-}U`aK|`VcRy%`;k#yKA&0E(_y"DQX;:0&H>R7n@(f\[8!YbnF#v10M?k)1mVeP *" }O^:gol%`MvO0Tl_U5TYe0tpJ_~7L3"6%4y^1Y03@a7&^LoT.iP$^=5NDzU46LD&YHE -kb+N4+0|k 1{sIRD8z{&]t[X.(<}rvP-R^;\wJ|*I*]E64Wg?UWU;j(6/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MS^ PBAS PBAS PCON ( PCON %v PDMF PDMF PLOC : < PLOC : PSZE ` PTYP ) PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT 7 RUNT
0 RUNT 6. RUNT
Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL u SPAC ArO SPAC SPAC ArO SVER 4.0 SVER Om SVER , , n Scal @ Scan 3 TUBE C1 Tmpr < User phAR phCH phDY big phQL c phTR phTR