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) . ! # & # ( ) - + & / ! & ) ) % * 6 E F F B ; B E = f Q B O K Z ; V D ; & - 2 / : * . * / ( * ) " ( & ( ' " . ' - ( . ) ' $ ! " 4 ' * / ' J , 2 C ? J U W _ R u l q _ z s { g k J L I I 8 / E < 4 3 . ( : ) J 7 = K ] i n e t l v t Y t x r W L H 8 A K 7 ? ' ' ) 6 ) $ ' / ) & ) ( & $ ( & # ! " # ) 8 . . F A 7 4 F T Z p w n | j ~ c b G C 3 3 # : 2 2 $ - " * + 5 5 2 4 9 : > H N O O K f e V [ d e n R C M X M P F ' 2 + 8 * ' 7 $ 3 ' " % ' * " / " % - $ $ $ % $ # ! & $ & ! 4 - 4 4 * . 0 , 6 W Q Q V U Y v p v x x u e a m n l h ` d W i g e h M K U > 5 C = 9 6 + - 0 ' , # ! " . ( . % 1 - ' + . " ) ( % * ) , 5 / + G 2 4 - 4 1 / ) , 7 * 9 ) < / - 5 % ) / ; 8 5 5 4 0 2 6 P E Z X f a g m ` ~ v d l x y h | u r r g b m k [ [ \ T C J C - H 7 ; & * + * - " ! " " & & ( ) - 2 ' . / 2 B = 1 6 M V M W T ] ^ c h f ^ B ] I M ; 7 9 0 2 ) - . % 2 - . . . ? C ; > \ K K b c k m z f m \ K H X @ / / , 6 . - " / * ) $ ( & % " $ ! ! 3 # . + . 7 < L V N L X k l w e j b x t i _ j c Y I E A N H > 9 G 6 2 . ; # 4 % ( + # $ ! & & ) ! & # # ( ( ) $ ( & . * - + " - % , + = . ( 0 4 0 0 ( ( " $ ) ! ! ! 0 , & ( . B = 6 C B D E S _ M N K R T K L P R [ R J = D @ ; : 8 A > 2 I 1 9 1 2 A B 3 + M U P 0 ' 9 0 ) $ . ! ' - - 1 $ " # $ " % % " $ " " ) " " * + / - 1 / 3 # # . 1 + 1 0 , + / 2 ! " % & + % # " * ! $
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" # ! ' , $ % - ' % , , . 8 , 4 ( ; 3 9 9 2 0 > A , > < = F B : A / 6 4 Q : 2 < < 7 9 5 ; 7 4 : 1 2 4 * 4 1 - 0 & ( , # & ' + & & % ( ' ) % / / 3 ( 6 2 * 0 / : G 4 ? M C M / 6 M > 3 2 / : ( @ - . d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 5V`deffffeeeedcca``_]\[ZXWUTRPOMLJIG M M M E E E a a a w x w %%%---555===EDDKKKSRRYYY```fffmlprrxyx~~~ 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 9 9 9 9 9 9 : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 22e3504058cc48b59650d52aac7e33a5Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-31_13-30_0264 3130Seq 12981 12981_Steier_PueblaGap4-5_matkF jmadera 96-Well K1362.G8_matkFKB_3730_POP7_BDTv3.mobSeqPop7z!/Eb .<FOYgy .K`mu{'251.0>Y|?tR2].IVY]fuz[.b$)rR0i f/aMNc
iSB6+ s\SXk
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KB 1.4.1.82018-08-31 15:09:21 -04:00AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS N BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % J8 x DATA
% J8 2 DATA % J8 | DATA % J8 DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ MR LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS ` P1AM % J 1 P1RL % J * P1WD % J 7O P2AM % J C P2BA % % J- P2RL % J = PBAS % % PBAS % % PCON % % ' PCON % % $q PDMF X PDMF w PLOC % J ' PLOC % J PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% X SCAN 3 SMED
SMLt SMPL I SPAC Aw SPAC p SPAC Aw SVER 4.0 SVER N SVER , , ~ Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH phDY big phQL c phTR phTR W This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS N BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % J8 x DATA
% J8 2 DATA % J8 | DATA % J8 DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab ]6 FVoc ]% FWO_ GATC Feat / / \ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ MR LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS ` P1AM % J 1 P1RL % J * P1WD % J 7O P2AM % J C P2BA % % J- P2RL % J = PBAS % % PBAS % % PCON % % ' PCON % % $q PDMF X PDMF w PLOC % J ' PLOC % J PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% X SCAN 3 SMED
SMLt SMPL I SPAC Aw SPAC p SPAC Aw SVER 4.0 SVER N SVER , , ~ Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH phDY big phQL c phTR phTR V"O`x83> u|#s\e?j(FsCaIqh?|cz2_'`g7FDKtRHSK%}jx7Z]et|?%9y-.^_Nx@rs{6`V5r@AKl&O3mjP/kdSP0i=qd)B*HU\A^O$['G=3/NwBx6_/<6R[Ule,rSBP^'6]`^ kS\KQp n$l^Wg#>z-nn[Y7!QnS,6dJX!Hiw8XcsG!}PlX87)*i`N-0Xwtej_@q3Luz>.9u2fkNM2y~'p9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&B2K4]J7U{GaC@LW&-3DLVzMymbERZpfP5!"{#[B]}P.Ea/YaWeDmAX,Pz;+hj3+'?762Z`K'HACd}Fb[q5FaT3"cX}49W+!'eAbru]ZH\J$4~%\WK\bynhbe/M#yKA#2rBArc{_@=KKR"\pI2qpWA~FT~~I!8U{~D@*Wi4GG\}d _o[x}sT!lZbCd]'wyJA}*4d#0Tl_U56_n&)-%TwKBTAq;vn}4|MdwYc_`.oU.Ja$*o][:0&MSDDBI^#.gh]3uPGlOOtvUVdy+W+oU{f4O(n`fP_UqN^Fu*tayr=u4|8e7s.FSKpuze/M#yKA[,5SZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaUFGsbrudrV3]NE^O{3fJ:h94aN{,$0K6!fJtb?eY?Dm\Ao!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72WeLWLVlcG`a[X?YCC/3[`kI ?"U27`N`3[)|$X[@5+W9]H2$bksR9QRu19"c.VDTj.uFr~o[|!Y<,h`%mI{Mb=KubQ2a5qO Jm}Pz;+hj3ST?0sKG'BNp3<("Hf.Q F3eG"k@j/.3]J&sCe#&\7`[qDr_WG\|J_j.u%xfmFoM/V,<{1z^{5te.ER]QZEpBoz?f.T|*1fCy]_^avep"iK]jSBrRL8~f)NKU|t2#71B!Hiwe2{sGitGK:Q&:C!g61CfF{\)lOY }YWoa$1pfnST/4f!Z56N|&ZG90Si! AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt AUDT l l kI B1Pt B1Pt BCTS N BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % J8 x DATA
% J8 2 DATA % J8 | DATA % J8 DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab ]6 FVoc ]% FWO_ GATC Feat / / \ GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE $ LAST ~ ~ MR LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS ` P1AM % J 1 P1RL % J * P1WD % J 7O P2AM % J C P2BA % % J- P2RL % J = PBAS % % PBAS % % PCON % % ' PCON % % $q PDMF X PDMF w PLOC % J ' PLOC % J PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
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SMLt SMPL I SPAC Aw SPAC p SPAC Aw SVER 4.0 SVER N SVER , , ~ Scal @ Scan 3 TUBE G6 Tmpr < User phAR phCH phDY big phQL c phTR phTR