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) ( 2 " ! & & $ $ + 5 9 . : , . ) 7 A 2 1 ) ( F R > 9 @ 8 G 0 ? / ? @ C 9 5 L G I c H S n a t z z l h c \ V ` \ R J M D 3 8 H 5 < ; * $ 4 ) 0 4 + % + $ ( * % % $ & / $ ) " ! ' > % % > & 8 - # 9 / . / 2 / - . 2 : 0 # % . $ - $ . / , B - 8 % > 7 G B 1 < a T e g c q f u p i n ^ Y a W V J : 7 K : $ : = / 8 * " # ) ! $ , 3 ? ; , ( B P 0 4 . 1 7 D . = A A ? < 9 5 ? > 9 % ( # % - 6 4 $ @ 2 - @ : * 8 ; > + H + 1 ) B 3 / P 1 4 & : : < < : 4 C C C R D Q W d ` U l { y z g s e Z W _ ^ M E Q 6 E 8 & - 5 = / 1 0 D , * ? N = : N A P j d c d s _ M V V \ N [ @ U : 8 0 : 4 , ) $ $ / 1 : ) ! ( 4 3 / : 4 < J 5 < 5 5 ( 4 7 2 9 2 2 9 = O > B 9 > A < I R V 0 L ; I Q ? A 7 ? N < @ F B = ; ; > X J W J H V ] g s } i w y w e e Z S Y O R > H B 8 H Q 9 3 > ? ' - 5 $ 1 ( @ 2 , . ) < / ? 3 0 < 9 > A 6 S J b k Y ~ p x d S [ d f U K < ? E Z 4 A K P = 8 5 B 9 @ ? - + 5 ) / . 9 8 3 9 3 + 8 A + 3 9 ; A @ * 2 # 0 5 / : . 6 # * / 5 @ * 3 = $ 0 2 ; @ / N I 2 - : . < 4 A $ : F 5 0 1 " $ 1 " & 9 2 1 C G A K X F P ] G U e u h h U ` = B J 6 M C H 5 0 B 7 ( 3 $ ' / * + ( * " " & ! 1 " * & ? - 2 1 ( 7 1 ( ' # 5 ) ' ( $ # 2 & 4 # % : - & ( 6 I 8 2 < D [ e Z h q | h j i } w c a [ K ] O J 7 ; ; % ; - ! ) & ' # ! ' ! ' # % 0 ' . A < , I & 6 3 / 9 4 * & $ $ - 2 , # " + ) & % # ' # - = 5 5 C R _ \ i z v } w | } r o \ m V J Q 4 U Q T 8 A ; 2 < @ ; 0 9 4 > < = B : = ; 9 J X O P k k X y | t h c g n X L a H C R C A 8 F B E L C C 5 / = 2 " 9 > ' 5 + ) 8 7 5 9 , G 4 * 0 % 2 * - " ' / ) 1 , + . / . # + ( $ ; : 6 M : : F G e ` [ f S [ R [ Z _ c K O - 4 2 8 " 6 8 7 ) # 5 : , - & ! ' % * 1 ) ? * 2 7 A B - 5 < % 5 / 5 9 * C 0 > I P 9 I Z S G 3 S M U a b j h h c _ o ` z c a ^ T P Q F @ 6 N C 7 = . * ' 2 ) $ ( ) " & ! , ! . # . ' * ) % 2 2 > + / O Y R S R S _ k z z x r ~ o q ] Q X Z X H L I B ? . G , @ A 9 5 ; E K 3 B Y @ X G C b Q < N 8 9 F 5 9 ; / 8 * 3 4 ) 4 ; # * # / ' % - - & 1 & - * + & ( ) 1 . 2 $ . & 2 # - 1 - $ # " " ) / , 3 & - ? 6 2 2 ) ( ( 0 0 " * + + ) + . ' - ' 7 ' & & B 0 ! / " 1 < 7 9 2 ; W J L Q N a S X [ R I Q L > Y C A D 7 5 . ' D = , + , 7 1 # & & % + # , , 5 ( ) % # % % " " - $ * . $ 5 . > 3 < ' 6 1 4 & ( . ) 1 ' , $ & 3 . 9 = 5 - N B G [ c N N N L [ { i P 6 = A : 7 & * 5 + 0 + # & # % & * $ , # ! # * * $ + ) ' + " < # 3 % ! 0 ( " & / ! & $ % ' # " ! 5 - " ! . ( / + $ ( 7 2 % 4 % % # . 6 3 . ( 3 A B / 6 ( ' 4 8 - + & $ - , 6 ( + 3 ? @ : : 5 I L \ X D O > V P P U Z I \ ^ d [ l l u { w p u | { x Z Y f W I I J H > 5 6 ! % , ! $ 2 . / - 4 6 ! ) ' ! ! + & ' 6 ( , ) ) ( % % ( + % # ' 5 " 4 A + 7 % F A 8 4 1 + & ' $ . ' + : 0 B 8 ( 6 ) $ & # ( # / + 9 I ; R W g ` j l y m } g z z s ` b [ R ^ c g a k m m n T ` m g V o f a R ` B E B F / 9 6 > % 8 * " - $ < ! 6 C % 0 & % 2 A > . . $ 5 , 9 & + 9 E 3 4 C C W O _ W ^ H X R I Y d W G I ` n L H K G N Y O < C B 0 7 " 4 $ + . ' & ( 0 ' * % ' * $ 2 - * 5 > 1 " 4 / $ 3 # 0 $ ' / + : A M D A J S H ; \ S F R L \ r G _ J 7 D > ; 7 * - - + , ) % 0 * * # % 3 % & # 2 ' " ' - " / & 9 " " " ' C ' & , 6 7 9 " 8 = : 1 * < K B W E O L J T K 1 A [ @ G / D I I 2 2 & 5 N 5 : 3 2 * @ ; 1 4 & * 5 9 ) + ' & ! % " - , - # ( & * - " 7 ; > / @ K K S O b ] _ p _ o W Z a ^ ^ 8 ^ S A * ? 1 ; P = > : / 7 ) ( ( ' , " 7 - 4 ! * + / 4 & 1 * " / 9 6 4 & - : * 7 7 : 3 ( 0 H W M H H F 8 R : ? E F I 0 : = ? , ( 2 - - 5 , " * 4 2 2 5 " F K ' E 1 2 B 2 F U X d Y J P ] Z V T U c [ ` 9 O C 5 . ? * ' # ' % ) 5 ! - ' / + ! - " ) " # + * ! ! * / , % ! " + & 2 # % $ " % ' 0 - . $ + ( % ( * $ % $ & ! 1 $ & ! $ * )
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/ ! + % 3 ( $ ' - % $ 5 $ % 0 J ? D ? a N R s a ` G O k [ L Y W P E > ; ; = O B B 8 < 0 2 % 6 ) 8 J C O S g [ M Q f o c u w } l o } q r v ^ l p i Y M g ` Y T R E B M E ; 8 6 < 1 / A ; = K ' G 9 C B 8 @ J 5 F B 8 7 5 @ E , . 0 > < 5 ' 2 1 ' < ) + 1 + 6 ' ( $ " - ( * ( , & ' & < ) + & & ! + * 6 < % * - ) " ) ! - ( ( * * ! @ , 1 & & # 2 # % $ & $ ' C , 1 9 H 4 6 6 J D . C 7 ? 4 B , 8 O G 3 9 T G T 7 2 F 9 A G 9 > E ' @ 9 4 $ 5 ) @ % * 0 . ! ! $ ( 1 0 $ * 6 5 & / 2 . 4 % = ' & 5 3 3 Y Y P X N U b _ a d e e w [ ` t q p f t O f J S R C R O B < J F E / % - 0 # # # ! ) # ' $ ( ! 1 ( ) * 7 , # , + ' 6 ? * & d ,,,III^^^{{{ &&&999DDDXXXaaavvv ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR 5V`deffffeeeedcca``_]\[ZXWUTRPOMLJIG M M M E E E a a a w x w %%%---555===EDDKKKSRRYYY```fffmlprrxyx~~~ 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 4 5 5 5 5 5 6 6 6 6 6 7 7 7 7 7 8 8 8 8 9 9 9 9 9 9 : : : : : : : : : ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 12799ae67a7b4043bc25ec2c2ca5d64dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-31_13-30_0264 3130Seq 12981 12981_Steier_PueblaGap4-5_matkF jmadera 96-Well K1360.F3_matkFKB_3730_POP7_BDTv3.mobSeqPop7z1R'$cXVY
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KB 1.4.1.82018-08-31 15:09:22 -04:00AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt r ASPt r B1Pt q B1Pt q BCTS [# BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & L x DATA
& L 5v DATA & L t DATA & L r DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ Y LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS x P1AM 5 j < P1RL 5 j 6S P1WD 5 j C' P2AM 5 j O P2BA 5 5 Ve P2RL 5 j I PBAS 5 5 PBAS 5 5 PCON 5 5 3 PCON 5 5 / PDMF X PDMF PLOC 5 j ) PLOC 5 j # PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% p SCAN 3 SMED
SMLt SMPL I SPAC Aq1 SPAC SPAC Aq1 SVER 4.0 SVER [ SVER , , ~ Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR h This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt r ASPt r B1Pt q B1Pt q BCTS [# BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & L x DATA
& L 5v DATA & L t DATA & L r DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab i~ FVoc im FWO_ GATC Feat / / i> GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ Y LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS x P1AM 5 j < P1RL 5 j 6S P1WD 5 j C' P2AM 5 j O P2BA 5 5 Ve P2RL 5 j I PBAS 5 5 PBAS 5 5 PCON 5 5 3 PCON 5 5 / PDMF X PDMF PLOC 5 j ) PLOC 5 j # PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% p SCAN 3 SMED
SMLt SMPL I SPAC Aq1 SPAC SPAC Aq1 SVER 4.0 SVER [ SVER , , ~ Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR Kj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:TxPQ1V'.$b=}$A>AR8d}$7|:zNz~vdlc*+?_u4ZfoQe=N^tpups'MvK}7V,2{+w" 4*39nbeWo0<:LP@]Xi-]js3W4KnuPQh^>kij7!}WQ`QEJ9."G>}l)-l&T|*1fCy]_^ave%e0MSaywKB3x[f9;"Y+Ax8aPI^QJH:,ptJdQex-U[dPzFy^q3rjWcgDkrD5pY-7cRb4/k=);$qphY3~HKTZamKcQ8p3s5ub(+gbk1^W7!_TcG,y"o-W2Bj:w`LJAU}WqD@~TlW+b@cT]`Aqg[A-WN}G7w,)=|{M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3<("Hf.Q+s3TtGK><3$G,lwd-l#frL?*Y^gM*y AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt r ASPt r AUDT l l w B1Pt q B1Pt q BCTS [# BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & L x DATA
& L 5v DATA & L t DATA & L r DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab i~ FVoc im FWO_ GATC Feat / / i> GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE % LAST ~ ~ Y LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS x P1AM 5 j < P1RL 5 j 6S P1WD 5 j C' P2AM 5 j O P2BA 5 5 Ve P2RL 5 j I PBAS 5 5 PBAS 5 5 PCON 5 5 3 PCON 5 5 / PDMF X PDMF PLOC 5 j ) PLOC 5 j # PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% p SCAN 3 SMED
SMLt SMPL I SPAC Aq1 SPAC SPAC Aq1 SVER 4.0 SVER [ SVER , , ~ Scal @ Scan 3 TUBE F5 Tmpr < User phAR phCH phDY big phQL c phTR phTR