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x d S R W Y J S o p y s r x z | ~ h s h s ^ m { x x ] Z [ [ < D 7 = - A K 1 K / 0 6 7 / 1 9 2 C 4 @ / + 4 B % + 4 * 3 # ) + # $ ( / 6 ? 0 < 9 9 O : @ C I ? Z e s x U [ h l [ G M F R X X n v k } y } t s x V L W E / M O D . * 7 4 : 1 1 - ( E - 1 F . 0 8 5 8 - 9 9 9 I C C Q U Y ^ l q r w U d N W J Q Y P E B 9 / H ; 0 1 2 5 J & 1 N ; 8 0 I T o r w ` f W I P H D Q D = / 6 9 ; C 0 1 - 4 2 5 > J - 9 1 , * + 0 5 3 0 ? 1 8 8 ) ' 2 A > . 5 3 = 3 8 $ # ; & 8 , % $ & 6 6 4 9 * - + ! , , * 1 . ) < 5 D A A H Q P R o h N n U T C = > A A = 0 + 8 : > 9 A 1 1 8 < G G ? V ^ N ] h v ~ y u t b q W R e k f c t p W ~ u o l y c n P S Y 7 B * . 6 2 5 9 F G @ P 6 9 S a K W w h h ] N P - . C ; 9 4 8 > A ( ^ / 0 6 + T _ X r Y e U H I H Y V U O P K T P O k y v f t z V c W X ; < = " 6 6 7 2 ' # , 0 $ ) ) 8 A # 2 % < & 7 / ) # 1 7 3 3 @ $ 4 ; 7 6 * 3 3 1 $ - J # - / " , ' % & 8 : 5 & 3 = 8 % 3 - " " @ . ? % " 0 A 6 , B 3 % % . : = 2 5 0 . 3 5 - 2 - 8 , ; > 4 > : 8 5 ? I = = 6 / / B 6 1 A = 4 K ? M N ` _ V e ~ o s [ _ Z a \ x s P S I U F J 2 P C ? P 4 0 " = 8 A 0 * , ( 2 / % 7 ( - , 8 3 < @ < ( / 2 @ . . . : ) + % 8 ' 4 . D K 3 > T m o s w { d e f g b V F W i R O d N S [ N R 8 = = H . 3 1 / > 9 7 . < > 8 4 3 > > 3 N 2 @ B L S S j g T G Q @ D Q K @ H I M N c t x j t h T ` ? 7 9 < L ; 7 3 0 D ; ; G A F G 5 B 9 - > 5 ) > L / 0 / 4 5 4 = 4 . > : 7 ; B . > 5 6 / - : O G ? N f v \ T ` k i J R @ H . ? 2 E R E 3 8 8 3 ? " / 0 . < 4 = 4 2 9 # 7 / 3 # 5 , , , < & % . $ % / = + 2 ) 1 5 / 1 - " ; + , * . + 2 + ' . 8 - 3 , $ 0 * $ - / > 7 1 K ! < 0 0 3 = : ( 6 : , - ' / 8 6 . 5 ) 3 2 0 + 9 ; 9 $ . " + * 2 / 0 5 3 C 6 , ) ' ; 9 5 7 : ) A ; ' 8 A ; 8 5 = % : R 2 5 ' P < 5 6 9 5 = - + 0 * 4 / 0 9 7 ) @ 5 2 0 4 G L , 5 : W ^ Y p o r n j a i z k y } d d e q ` q l q ^ q b _ a K P E J A N C 3 E < 9 L L I _ V r x z f V Y E Z O D = ; 1 @ F : 5 * = 2 @ : 2 B C . 9 9 K 8 / 3 - ? B > 8 ? < ( 4 - 3 < : / C C M l O s ~ m f m b e } a h ` h G E R D : = > % = + # / 5 1 : = - 5 8 3 % # * 2 0 B % 1 : + 5 ( $ 5 3 6 1 D A 6 5 G , 9 < ( 8 , @ 6 . H & / 4 ; ' 4 = 8 . E / . : / < 4 8 H = - / # < < 9 % 8 / 2 D 1 2 3 3 ? - S = 6 > 2 3 0 - ' 0 4 7 4 4 . 6 H > . / C I < + =2 < ' ? * : ; 7 T 4 = 9 1 6 ; T R P c g p d ] [ T R C Q M 2 N 7 B < Q 6 0 < > 2 - 3 # 6 2 < 0 , + 3 B 0 = = 7 9 < 4 ' @ " A J : 3 > ; ) @ 6 L C G / A T G G M ` Y n \ ^ e z n m \ x l m ` { x g r e i t q t a Z d c Y 1 X W U = H 4 C Q 2 I L J / < M C J 8 > < = C D 9 H Q @ W H h t p ^ Q f N C N ^ O @ > C A P B J I C @ L A 7 / / : ? & < I / J 8 A R < 9 S I P C L Q a ~ | y e ~ g X ^ ^ k 6 M V C > ; # = < E ? 5 6 F . / D 7 ; , - D / 2 4 @ " * > $ 3 B C ; 6 3 7 7 . 0 9 6 , D + 5 2 . * C , 5 - + ( ) 6 0 2 0 + + < 4 : < 5 4 , * A 7 ' ? 4 & / 2 2 5 8 % 6 2 2 ' + 2 ; ? % + $ 6 ) 7 . # : ! / - 2 : 2 * : 1 . ) , 3 2 - . D ( 2 ( ) * " - * * 7 2 / ? 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 638c0706ee704d03a56edef30f471cf1Seq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-31_13-30_0264 3130Seq 12981 12981_Steier_PueblaGap4-5_matkF jmadera 96-Well K1356.G2_matkFKB_3730_POP7_BDTv3.mobSeqPop7z 2 4 8 @ K X b f c ^ Y W X ] c g g c \ V S W ` n | ~ q c Z W Z a g h e ^ W R S Y ` g k j f ` [ Y Y ] a c c _ Z T Q P R U X Y X U S Q R U Z a j u } { ):Pi#;Wv!/1)
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KB 1.4.1.82018-08-31 15:08:53 -04:00AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Q BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % K x DATA
% K 3 DATA % K ~ DATA % K ɮ DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ Pn LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM ! B 4E P1RL ! B . P1WD ! B : P2AM ! B G P2BA ! ! MM P2RL ! B @ PBAS ! ! PBAS ! ! PCON ! ! * PCON ! ! ' PDMF X PDMF PLOC ! B ! PLOC ! B = PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL I SPAC Avz SPAC SPAC Avz SVER 4.0 SVER Q SVER , , ~ Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH phDY big phQL c phTR phTR '၅ This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS Q BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % K x DATA
% K 3 DATA % K ~ DATA % K ɮ DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab `Q FVoc `@ FWO_ GATC Feat . . ` GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ Pn LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM ! B 4E P1RL ! B . P1WD ! B : P2AM ! B G P2BA ! ! MM P2RL ! B @ PBAS ! ! PBAS ! ! PCON ! ! * PCON ! ! ' PDMF X PDMF PLOC ! B ! PLOC ! B = PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL I SPAC Avz SPAC SPAC Avz SVER 4.0 SVER Q SVER , , ~ Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH phDY big phQL c phTR phTR -'Icf]h_m7^1V .Uw.PmaSj4q+E[7VGJlqbQ9dHUslGW[#xjT|T6q32&L"p;I`NjYid{0PajeoI.*R@_aA+|1hygSHmT;P6[s$\o}Z'BNpW8:7F}wxSB#_{ykI0ouNbv&~^\mcJTGyr{[Kl_e5 }37RIBWjt(mID9`hp}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9)]ZlB2K4]J7UZGfC@LW&-3DLVzMysP!b`}9(2&Sy'"jet?[)1-lHLl}gF$eD:91VuT(A|!Y<,h``z&^7a."sWir;-rXN:!)YWFwLPmg^Bq_6k99/qNE^O{3fJ:hA?I| )TuTF}T\Jpx _,y".9W2Bj:w`kPhL`A(^:go8m+adJs _ZZ^S_ngZ-3$tir"8|oTq}A|1D@<7>{AXG9{$5t{`QPdlpXbVclmGn(?$OA2nrD5pYlGcRb[q5FaT9Vbr$rv/qNE^O{|cHT?Ou4|8e7spFp[O6h"4#O|!f|bEdG;_{8@hh/WZ#3|)l>,S]ZD>tSEH$*YYo%+0D5p>?e t}1%jF}up"GrM7VpCJT|Fu*qcjhAhE;qT%;5]U' S!oS\[Z*YaRsgGw>2Mcr.Lk@_aP4rRj"SD=y!.qK|N$t.DkFhwD~EG7&o.AiG3mwYB{,9)-]HqQIxw}H{-*c{Xm|3HF)SE8n\qo/RH&udrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gM AEPt 3 AEPt 3 APFN o APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt ASPt AUDT l l nd B1Pt B1Pt BCTS Q BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA % K x DATA
% K 3 DATA % K ~ DATA % K ɮ DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab `Q FVoc `@ FWO_ GATC Feat . . ` GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ Pn LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM ! B 4E P1RL ! B . P1WD ! B : P2AM ! B G P2BA ! ! MM P2RL ! B @ PBAS ! ! PBAS ! ! PCON ! ! * PCON ! ! ' PDMF X PDMF PLOC ! B ! PLOC ! B = PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL I SPAC Avz SPAC SPAC Avz SVER 4.0 SVER Q SVER , , ~ Scal @ Scan 3 TUBE E1 Tmpr < User phAR phCH phDY big phQL c phTR phTR