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L E c 9 < : 8 @ 5 B I = E < 4 K L = C J < X O Y F N c a a I N h h q \ I M U m s Z N R W [ ^ G G 4 E , L F : F J 9 B A B M C I G ; G = A D J L Y e { q s w c a p ^ ^ K G O > 4 < 5 , > A M ? @ D E @ < @ B @ F H > B ? F > G A P F N W i R c o f ` R e P Q = X D M I C E 7 P M ^ Q f h Q [ V e T < Z L D R 9 ; > 8 4 @ . , 4 < D / B / 5 A , * / @ < L F Q E G D S T U D F S S Q R J J V _ e m V e h j l d Z [ ^ P W ] s { u r a \ X G I E ? C * Y 9 J S 4 R A G V B O P R Y ^ \ a V r w q \ a [ J R U g f c p t z x } y v W e s k j o b i ` f E H I @ ? 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N C F H > W _ V g p e o k l r w i X T Y Y d [ ^ i W f Y s ^ G M Q L e ` ] \ ] ` s i j h q a s ^ O T a a ` i ] ` s y p Z H n j S X K h j i \ p ` v m f R S d b d i e h a | y s n { c \ U V Y > F ; A 8 . 7 @ 4 O = 0 3 A 7 6 4 F 9 / / 8 Z F 0 7 E G @ @ A R 9 9 9 P R Q X j f a f ^ _ m p m ^ ` [ e b P S N L K J 9 Q > 9 F ) E B < > A E P E V G ` e a f x v ~ z } ~ t c _ ` K Y 2 / 7 6 E A > ; 3 E @ 8 > = , 6 4 H 0 = 8 5 B T Q S Z V Y h g g x f W M P @ X ` G L \ Z c m e f \ O P Y T O U G G I D ; 1 H S U = I @ F M D = ; D C 4 B B E ( 9 D F A 4 1 - C B + G C ; 4 F > Y \ S P H f a d X T a ] a A Q G K @ G D ? H W U F < 2 E ? A I I b \ C J > K K I 7 @ > C ; ; / 4 4 B = D & > C > G D 3 7 ; B 4 9 @ : 1 5 7 9 C ? 8 6 < E ? A , 7 D > @ < ? - 2 B : B L K C A A 9 C @ : O I Q W [ V b V ` [ ^ Z U W U \ Z b r a { ~ } v { o X P R F ^ P ? B \ N V L d \ o g F ` h T O E Q L O ; < > G F > Q 1 C J K T D c \ i b d d ` e ] U ] Y J a [ Z _ Y j d h ` ` l p _ y o r z e ~ t w m d a a W X [ n e i d e w h i o ] R N Y L E I Z Y O k i Y Z V _ e P Y I C N @ , - B 3 I J K I M L 0 8 ? 7 C 9 E 2 F A 4 4 4 > ; 5 B A < A A H A 1 C G O C S 3 B E E < 0 7 N J L D Q E F R G D C ] a > a _ T w Y b V R D G @ 7 N D 3 8 > B Q < D E L F @ A ? O B 8 D ; : = = K = G / V S K K ^ M d \ g j r i X v N R W J K K @ O A I W 4 V R R 8 H 9 O K H J K @ N : V C J A N M P X T ] d a b h z p k e } l y ~ i q l h e f v r a [ P Y < I ? G ? A < : = F D = H N O R ^ e o x x v h u n x x Z c ` k r X [ a W V V q ] H ] O K > E < 8 1 ? K = @ C O \ T W U T e k _ l h v c g f N S m ^ e ` q ` ^ c h V ` ] J ` U N N I L ? P G = M Y C N G b X E T \ d ` f d o W f ` a P J R = : = I X ? 7 9 5 K 7 > E K > G U J Y Q S [ R R ^ Y Z \ b R i m f m t t u m b Y ` ^ O ^ C R R a c d k _ g V i L k G P > T D G C M ; K < H P E V : + E H X N P L g m R ] N R Y E N ; : > 9 D : L I ? Z F . 9 5 = 9 3 V G O > D A M C Q O M 8 H E H 9 8 > 0 M M N 9 G 2 8 A L ? V A D E W [ N P U X [ N _ R _ l ] x ~ y p u z l T c V ] M \ F [ K c O N f i a ] k ^ W P N K E P A ; D @ A M H P C N E H @ N 9 J [ e b Y i m n m o v b Z U L Q T < C S E D Z C W . F ; @ U S Q ` h ^ Z i u o g ] d _ K ? I B P M a W d T P \ [ f M G _ I G E G J J 9 J G @ @ D N D > C > 8 T T ; O O _ S X e T l q q g l q W a n L K W 4 6 P ] G ; C = M L J B 5 9 5 9 3 > E C G S I K R W N [ \ _ S c ] ^ J F H Z K E > J Y S S K K L G f a \ a \ \ I A M T D ; 5 8 ? D 8 K C ? 7 H 8 = 6 5 ; G M 2 ; < = 7 < E C B @ : I F B L < F R L E Q C J M E D T [ > C H L a W P V t V p | t h q S U X I M T A ? H 6 B C 8 ; G ; B < > E ; A Y I X 3 A K K > < . @ = H Q @ 3 H Y G O N - @ B C U B A 2 = : < > D C 1 9 N : 7 N : G D B ? D 3 5 C < O I K > I O Z U f X N X X Y Q R W V N 3 K D ) 8 J E : 7 < 8 > 5 > E G 9 F B [ J S X d R I [ L _ = B O Z T ^ k S T N a Q Q h [ 7 A L 9 O < 8 I M ; N D M @ R P X 5 ? G E M J D C 4 H K M B 8 < : N A < P < C B ? . 8 U I M 5 F ? @ D J E ; A W B E M < B > 5 F C 7 E : 2 < F 3 A F 2 5 F < A D N Q < G Q ? H @ : A A 9 F j J { Y R a R O S D L = W F G f R X Z Y ] X X [ W O P = H A O 3 3 E B I C 5 U < 2 1 N U \ U g m h \ ` k c ^ Y ] d B < ? 6 E G 3 @ C : = 4 I M I O T Z W O c R k Y T U > A C B H H : 4 - O 7 A F @ H B M M F I B 2 < Q J P H J > B 9 R R S H R c M V g m M q k Q ` N V E E J R Q D C M _ _ X R F M M W O A R @ I 6 M C > = N = J B = : > M > 9 Q A I R 4 E J ] G ? T Y L X U S g G \ J R O T @ J 8 C A C F I C A 9 4 T 7 0 8 7 % 4 8 4 7 . * 1 6 : 2 8 3 : 2 4 ? C @ W > > 9 N S O D J C B @ E D : X . A / C / 6 = A ; < : D - ? S : Q N B 4 : A G D E : D < H @ N 0 0 A E 6 I E _ C A M W P P : D < Q K J S F D @ T J [ M u a ` e [ S Z a X V V V ` q f k k d q Y Z L L B R W P R j X ] n _ S c Z g W X Y _ \ ` n j i d g f ` X 7 i Q G V a S O F S U S ; I L E K G E I O C M [ U b _ W o [ U d P \ T [ k _ m a q k r q l Z ] Q Q X G [ a R P P M R H 5 ] N S W Q ; E O L I M = A M G F I T M H Q J L Z ` X U S N L R C 9 H L W O S @ ? S O = J K J 6 G > J ? @ A 2 H ? I B K 0 ? N E A C H F , 4 - B H 1 J 9 - , 3 K M 5 ? C C 8 G 9 1 4 J = D , 6 ? > ? = E = R 3 D Q ; ? : C ; H @ < U K R _ J ] i V L b Y F L J < R ; 4 G A B U L ? E C 9 C K G * 3 C G @ 6 7 ? / ? 3 B B 7 E ? 2 4 < 8 ) D @ 9 H / P = @ L 8 H I M X S ^ Z Z X X P @ H Z ] @ M D : A O @ 1 - 3 B B A ; E < > < 3 < C : : > 5 G Y P Z D J a ^ V ] P R Q H K \ O O ; F > > A C 7 C 1 G L B I N V \ W v c U Y P W Q W I M Y \ W L ] [ H ` ` X a J Z I ? H C @ : O 0 ? : ? 6 7 B . = A C 3 + F 4 A 7 3 : ? E 6 / * 6 6 C " C 7 A E 8 R 2 9 5 ; = / F E 5 , ; ; / J F < = H F < E J A G F Z L W G [ \ P R ^ ^ N M [ 9 < : < 7 ; 3 3 C P U T B L R a e V X N i Z S W J ; Q E K J Q D b T U G N J C G D W J ? M > 4 I E F , < J [ Y G W ^ d Z Z ^ Z ` N T T N L B / 7 ; 3 H K N : C A : 4 7 G A 4 D < @ * ' ? Q 7 T < 0 D G M \ m \ w p u x s u w m l S Q V d O F J 7 + A A 6 9 Z < 9 : K A O . G 4 J 9 C 9 C L [ E O d f ^ d N ^ [ I ^ Y J H ] d Z R N V Y ^ U U ^ H L > E D @ ? 7 D : 9 F > @ J < 9 = G G Q Q W M P K i J N U ` B @ b J L Y N X R Q X _ _ a Z a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i K Q A P J = 9 C C / < . G 9 - 7 2 2 < I I 1 G J L @ F 7 9 E M / = B 4 ) D / 8 ; 4 4 ' $ 2 / 8 : 7 3 = A 6 : > A - = 5 E ; 5 9 0 7 / E ; F < 1 D : . H 4 5 - 7 - = ? J < 8 - , 9 4 4 9 / , * 5 < > A : + 8 4 < # 9 ! 1 ( 2 + ( ; * + 9 7 ; 2 ' G 0 4 . L = K G N N A > > ; 5 4 > < 2 R G N 4 7 ' - 0 $ / - < 0 : = 8 . # ( . 8 7 + ) : + ! . 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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3d6a7ef291b443baa0a864b844ac058dSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-31_13-30_0264 3130Seq 12981 12981_Steier_PueblaGap4-5_matkF jmadera 96-Well K1356.C10_matkFKB_3730_POP7_BDTv3.mobSeqPop7zDSt
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KB 1.4.1.82018-08-31 15:09:41 -04:00AEPt 3 AEPt 3 APFN p APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt { ASPt { B1Pt z B1Pt z BCTS F BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & M y DATA
& M 5y DATA & M y DATA & M y DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ E LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM @ 1 P1RL @ - P1WD @ 64 P2AM @ > P2BA B P2RL @ :t PBAS PBAS PCON + PCON )t PDMF Y PDMF PLOC @ %4 PLOC @ PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% y SCAN 3 SMED
SMLt SMPL I SPAC Am} SPAC SPAC Am} SVER 4.0 SVER F SVER , , ~ Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN p APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt { ASPt { B1Pt z B1Pt z BCTS F BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & M y DATA
& M 5y DATA & M y DATA & M y DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab T FVoc T FWO_ GATC Feat . . T GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ E LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM @ 1 P1RL @ - P1WD @ 64 P2AM @ > P2BA B P2RL @ :t PBAS PBAS PCON + PCON )t PDMF Y PDMF PLOC @ %4 PLOC @ PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% y SCAN 3 SMED
SMLt SMPL I SPAC Am} SPAC SPAC Am} SVER 4.0 SVER F SVER , , ~ Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR k]hiqU"T;#6Rl7U?D0^k,h/fP)} ~&]Dn?IO?p[X!m6ul0${N|X-N&W-dOsW&wzl+<,,pCws^D\we*S#ibu@QhCe3fGX.!6k|TIx*&`cz_GbJE6=W!(/tj glOM5cWL N*hbDv.ItJ[DIBG(XX7&EUU=:+i,S#|n[MVCE N|Xe1oH/`Wa?iITwM"f^@/(y8|o5c;9Vge!Yz]/d*sd6S<;1,;P}J&,39:8 !r|XquYFo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEe>2fkNM2y,'~9'+ez^.-QlMaP)BuWZr%"[ T]Wm\y}4r(>8~Vz7}0eq/V-AMV^@5oOC&qq<6(m=':Z^u4|8e7s+]i@CHbBzH+6-w/l?:Z`UEUi?ErJ/N'4;M&bX*F8Bhrb8EY|3.B+0T{!Xf] _ocT)FTX>j3XV=K3]1wv;{?kJ:h,r4[-T0M5[S*.J-j~W7.5k|_-S_7}_ooUzbaI23|5njUZ'KD7I 9xxsWD{xvrL>DI6pebvM<$AJT[Mo"$+r:y`/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L(\_sW((bw(nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_M%_|_n?\h =&e=1&!rE_g4YDI#%+ AEPt 3 AEPt 3 APFN p APXV 2 APrN g APrV 1 APrX } } p ARTN ASPF ASPt { ASPt { AUDT n n c
B1Pt z B1Pt z BCTS F BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA & M y DATA
& M 5y DATA & M y DATA & M y DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab T FVoc T FWO_ GATC Feat . . T GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ E LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS P1AM @ 1 P1RL @ - P1WD @ 64 P2AM @ > P2BA B P2RL @ :t PBAS PBAS PCON + PCON )t PDMF Y PDMF PLOC @ %4 PLOC @ PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% y SCAN 3 SMED
SMLt SMPL I SPAC Am} SPAC SPAC Am} SVER 4.0 SVER F SVER , , ~ Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR