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C , - 7 0 - D ( 1 H 1 + - > D > = 0 > . 9 1 ) ( * 0 ) . ) + 0 ; ) 7 % * 5 7 C 2 * 4 3 5 6 , 1 ; $ % 3 > 8 ' ' 7 6 8 3 7 4 M / 4 , 5 . , 1 4 ) . 1 . 6 = # . 4 * + 3 E = ; F # 2 4 2 ' * 2 * 9 ! 6 C F 5 G F Q D r ] a a i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a W T G A : I M < ( B P - 5 @ L 9 ; > b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`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` e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d b W A f Y N P U : 0 4 < @ 8 @ > - & 8 F 3 4 B 4 F > 9 6 9 , 0 B 8 2 . 3 D 9 J 1 K X H W [ S D I D E T @ G @ 1 7 ? A 9 , 9 ' 7 9 1 : $ 1 : 1 + 6 6 , M < ' 9 B = 6 2 8 " . ? / $ 7 , . 7 B > < : A > = O R L O b _ Y W d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Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 622dd26a8a074e42ad5f40745221862eSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-31_13-30_0264 3130Seq 12981 12981_Steier_PueblaGap4-5_matkF jmadera 96-Well K1321.A2_matkFKB_3730_POP7_BDTv3.mobSeqPop7zaE(eoCEx `
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RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% ! SCAN 3 SMED
SMLt SMPL I SPAC An\ SPAC !0 SPAC An\ SVER 4.0 SVER >! SVER , , ~ Scal @ Scan 3 TUBE C4 Tmpr < User phAR phCH !N phDY big phQL c phTR phTR 2I\(f^t8 e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0Fk:@u)(\`FUvgNGr=ylDbaxJK[Yle9Su3]NE^O{|cHT?Ou4[42g|5(T3#T]TcM#yKAc,2/jzJ^QJH:,p""#]eRWJT|eiB]%j'kXI"ggZiOHkij7!}WQ`QV-K[YJ(V]AmE$w^6h7fcT%GY?tYsYbk11&OJ-sP!b``ye2&d\wmj7`;L b9A?aeD{$maDDy*r`snHo*uhGlB^PY6C}_lnqR9]]?EZp9}:>Ju6v;(vJnqG\buk'"5-z]G3o6Rl>Mm[*&`_M%0g'^+oL.SVX*os7}Z&['K2Fy^q3rjWcgDkFT16Y=*"9.b':8 v/#wCdNmbcnkE=+4[q"CsINi@C,C12Mm|!Y<,h`\wmj7z3bj6[/3-6)SE8pT/@MOsijd(DUfKkm%p!.b@\W}@K.]R[gF{\)lOY@ZWeoak"T'o0_s2#O|!f|bEdG~_ {JH:,bQ2a5|OjAZeXVm? >S]T'_C:Or4^7~s?K90lV)XHT1yqX6\qwH=qd)B*#k)lO[vHd55-z]T1jzLx2&,x}ka03(_yb;E26?^V$Yeaejp~K]yDWUhcH[X]Lu:ZqLNDI 7&o.1r9v[?|$}~?V'i~?RVuU,H/`WajZUTw|(F}K7T]T%E+D0.98xOrz6 ?63_1bTio0, BufT A CTID CTNM CTOw 1 CTTL 4 CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA ^ DATA f DATA n DATA v DATA ' N( x DATA
' N( 6 DATA ' N( DATA ' N( DCHT DSam DySN Dye# DyeN DyeN % DyeN * DyeN / DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT Ī EVNT Ķ EVNT EVNT FTab L FVoc Lv FWO_ GATC Feat / / LG GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE LAST ~ ~ < LIMS ! ! = LNTD 2 LsrP a MCHN MODF ^ MODL 3730 NAVG NLNE ` NOIS ! P1AM p / P1RL p , P1WD p 2 P2AM p 8S P2BA p p ;3 P2RL p 5s PBAS p p " PBAS p p !S PCON p p +c PCON p p ) PDMF X PDMF !7 PLOC p ' PLOC p $3 PSZE ` PTYP 9 PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT
RUNT ' RUNT RUNT ) Rate RunN " " S/N% ! SCAN 3 SMED
SMLt SMPL I SPAC An\ SPAC !0 SPAC An\ SVER 4.0 SVER >! SVER , , ~ Scal @ Scan 3 TUBE C4 Tmpr < User phAR phCH !N phDY big phQL c phTR phTR