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(  $    #                 $                                           !'                      !$ !"($$ 2! #                                               -)!%  *'-$!0"              )   --&&% $# "               .                          %         %"  (                                  '%                 '                                                !                                                                              d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5V`deffffeeeedcca``_]\[ZXWUTRPOMLJIGMMMEEEaaawxw   %%%---555===EDDKKKSRRYYY```fffmlprrxyx~~~22223333334444445555566666777778888999999:::::::::;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9f068e6350004bbbac7444d26a8fe219Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-31_13-30_02643130Seq1298112981_Steier_PueblaGap4-5_matkFjmadera96-WellK1315.A10_matkFKB_3730_POP7_BDTv3.mobSeqPop7zK<~lu(yD-q 1 K r F # Z#s;?T #('"uuWZL O  } 5 g <^VezvRxGMn,yeI, &(Awv- } QWX!"#Z#^"! 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R<PFKZZ,L80X#/ b^\4F4C\J7Z C3N 86"1Z<;'/B:,8V:Y 'NA94S Xa 6X^2 )T@8$ aR^=W2DL]>>SZ%B (X'99d0a79W;("XHP/! 4A[WM5*<DH/ Y2YaK <V?]W4(VM 1 (Qq6um tRe9b+ Fh 3  5 )! kQ$<A!^,cTT \Rd 3dY:/%tLX !8. ` lL.0DonA'ucQ=5w s0?YMV[RcnDJ&bwGBX1!`7~@+G3[hsvB|YV8q7Hs91|'s?/a]Fv cDs_[ @_eW8w"o?].hx~jyG $xO*#1*uIXShyu=2e[}~@~K^ c5b{JF'^?gJ4\U> )SCHI\ YAkjSV TqyS!Qes}tuM8xk,x=^4L# _SCxKz $_dc6cj +/<g  8b: 6NE"F>)I$ ;, ?+%' # .L3 7L6)mIQ*4 Mw,py8pap_m `&}F&O! $UP,A1 [(~Az K.sk -2m,NXdn5? dNv9 58S?&*=8K8$ ,Qn](/AVk"NJ?I7Jg>@=K#AOXt= fp-f\TD;[g8oWv4":ldKN&W)NY;/>dd.pcf<T76P, $c,a8920:&=v [;0# M]7Y['! )CrnvFy$~(b>l2 x&X'4Os-d<LhJ_Si\,Tf9clb+q7gw]$F|Z)Ud=BaF q)[2IZ! z^Lx#WEz>m&zVqEaH)xBaxwHn9)bF+Y,z`]B3?yly\edov!,>?|n[f#x=B[H4n>\hU ori5]9O# | I/WE1sU1 m6qm_ pCATATATATAGAAAAAAATATCCCGTTAACAGCTCCAGTATAATATAGAAAATAAGTATAGAAATAATGTCCCCCTTAAGAAGGTGGTTGTAAAACATCCCCCCTGGGTGCCATTTGTTGTCATGTCCCGTCTGTTATTAAAATTCGCGGATAGTCGGAGTCCGGTACTCCGGGTGAGGCTGATGCCGGCCTAAACAGACAAAAGACCTCAACCGGCAGGGAGGCCGCGGTAGTTGTCGTTGTATACTGCTCACGTTGGGTAGTAAGGTTTAAACGCGTGAGACTTAGTTGTTACTTTGCAAATACGCCATTATTACCGTAAGCGGAGTCCCACGAGAGGGTATCAACCTGGGAAGCGGTAGACCCCCTCGTTGAGGCGTGAGACAGGAAGGAGGGAACAAGGAATGTAAGGCGCTTTAAATTCAAATTCATAAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-31 15:09:48 -04:00AEPt3AEPt3APFN pAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTS:BufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA & LyDATA & L4DATA & LDATA & L̯DCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~9ELIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AMf)P1RLf&P1WDf-`P2AMf4,P2BA7P2RLf0PBASPBASPCON$PCON#.PDMFYPDMFPLOCfPLOCfbPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACAswSPACSPACAswSVER4.0SVER :SVER,,~Scal@Scan3TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTRꁅThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN pAPXV2APrN gAPrV1APrX}}pARTNASPFASPtASPtB1PtB1PtBCTS:BufT ACTID CTNM CTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA fDATA nDATA vDATA & LyDATA & L4DATA & LDATA & L̯DCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ĪEVNT ĶEVNTEVNTFTabI(FVocIFWO_GATCFeat..HGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE@LAST~~9ELIMS!!=LNTD2LsrPaMCHNMODF ^MODL3730NAVGNLNE`NOISP1AMf)P1RLf&P1WDf-`P2AMf4,P2BA7P2RLf0PBASPBASPCON$PCON#.PDMFYPDMFPLOCfPLOCfbPSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT  RUNT 'RUNT RUNT )Rate RunN""S/N%SCAN3SMED SMLtSMPLISPACAswSPACSPACAswSVER4.0SVER :SVER,,~Scal@Scan3TUBEA8Tmpr<UserphARphCHphDYbigphQLcphTRphTRKj5)l#O[h?Yo%"*"i/2Rd}/o66Q^_4e]"=v1r:zwpF*xvJ\S#!ZI@])HJV1pfnST/4f!Z56N|&ZG1&w%:T?1bTio0<57#u?GG5WriD$[Wtua 3nG6`V5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B`>-?[G2upkyl,7hhSc>6?|$lQ%e+Bw9#MBz3)-l'CFbhLYr4EjuDs5iqbyMimE%ToN6|1:#<'r{?:TJWT|+adJsLl&pvedsgbk1^J.(KC4!7`h?&03BSmv^*:U[*WXWo0\,$bnsYK%FyPq6!oSR{[}7&0AS_7WX773V[R"\"@-bWF'zPz\]G!3@*7_6; 0|&6X`??}@8jaA]!lZbCd]s:goT|gmktayr=u4[PngD]jTj.uFr~o[|_&agD~jg6j{th_B$e_4ppOxU&g_n&J;s$dUW@_G0:jiIvr$c}Wq=wM$^{y?hA(ue6bZ( ]'yZUogU7B!XwKcUW0gK >5(ePX!Hiwe2{sGi%ycX}OPmE$ww*{we*.l_pD8J)RnLHbcyShKn6g;X6YMFBj'~;H26t