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"                                   ) +  "                                                                                                                                                     d,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5eee0b05b9d44c1183bbcb8c07fc50b4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1364.E1_psbAKB_3730_POP7_BDTv3.mobSeqPop7zU.V 7dHr{cQ<AA {\= tZLGHF=- .9<80&   !  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':JPK;%)31$ ! #1870%  %02,  096+   -FWYN9"""  '4;8,  ((! *BSVI2   *1/%*9>8*  (36/" /BKF5       #'$   !  $(&     $5>:, *0-"  ! $&    1CR[ZN:$   #/1* ).)   )592$    (1.#  =Wc]F& 4NZU@%   !  *0,  *9>5""&"  (:IPK;% ! !4>9( #8FF;) '+(   $*.12210-&>eb9 +-# 4A@3 ".0&     *.* #:MUO=# 2AD;+ %<NSI4);EC7$"+,$ +2/!.:9,  9OWL2(:>0#E^eX=     4FMF4 #3<;1   ,2/#  /@G@. .;>5$    '*(   0:9+"(%     >ZjgT6 '6>>6)  '-) #296*     "074(/DPOB,)-( #*&:Vc]E'   1KXT?!)66*  #    !&'" (58/  #&! )591#  (34+     #  .?GC4!,68/ !8IME2%7A?2 ! 4DJC1    &7A@5# %+' $<MOA(%+(   8MVP=$ ,CTXL3/?B7" /:9. .FSO<! !.0&   "$   '0.#-=A7#)6:3% (Kjzv_? "5BC8%0;<3#"3>?4!  5FKC1 8Q\VB'   1@F@/ '=LL>( -54*   %/441,%')# &,+&  1HTN8 :T`YD' $297+  087,  '3>C<+*8:0     %)& #-1/&     3?=0  -=B;* %7BA4  &*+' ).-+,0695) ,56, !173&*5;8- .=B;) 2;7) ';HH<) /=EFA93-(" :Uc]E& '/*     (10$-2,       "**#     3KYVD* +43)  +DVZM3-@G@/    &7?;+$493%   4IW[TE5&  $-0,# $$    ?axzfE!  *G]dY@#  "%!    &-+  +12-$     C@ AAWAVKB.bcpKB_3730_POP7_BDTv3.mobtermGTGGGGTGCACAAATTTTATTAAAAATAATACGAAGTGAGAGCTACCTATTAAAAAAGAAAAAAAATTTGGTATAAAAAAAAGATAAACAGGGGGTGGGCGGTAAATAAAAAAATAAAATTATAAAGGGGGAAGACCATAATGGTGGGGTGCACAAATTTTATTAAAAATAATACGAAGTGAGAGCTACCTATTAAAAAAGAAAAAAAATTTGGTATAAAAAAAAGATAAACAGGGGGTGGGCGGTAAATAAAAAAATAAAATTATAAAGGGGGAAGACCATAATG !(4;MV`jry %0@KUairz)7?HOZet&2=IRZap} #/;LUcu,>JXfo 1;K[mx !(4;MV`jry %0@KUairz)7?HOZet&2=IRZap} #/;LUcu,>JXfo 1;K[mx                                                HJab_<?7%T@8E"=@3b K"@ "*CW"SMC5__ P2F- //9A>[J.G/H7b ]6 8I!>TC&(8?)LS"& U<5+ _ W0R(K0U9\9`DX8T d=8BPc_P_T9P CX- ? O HO8# 9 L  a ( l  M+%TH_4!UoR IFD<)aodMxpQo) XE: =R?G"[K.x->[ 5 u-.]o]9Vs)B$ N _Z ) d=mX0cL)E/k As .O23J&HmKQgHLDcBdqlA} 3b{(?9cc8d,p(,p,j+.mp47,A 'xe@ek>2pQ%9lz=+I cC, O- !H@. :# Xeko}!6tp._Ldv}yb(&| 88)ON%i?Imwmu)s{F(LHL?2:X* uwBzXTGTTTTGAACACCTAAAATAATTTTTATTAGACGTCAACCGCTACAAATGATTTTGGATTTTTGTTAACTTGTATCTGTTTTCGCCCCTTTTAAAAAACTCCCTTTATTGTTCCATTTTAGCCCTTAAAAAGGACAACGTTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:26 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GjDATA #Gj, DATA #GjsvDATA #GjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~ "LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISRP1AMP1RLP1WD 9P2AM uP2BA P2RL WPBASPBASPCONnPCONPDMFPDMFiPLOCPLOCPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%JSCAN3SMED SMLtSMPLtSPACMSPACbSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtAASPtAB1Pt@B1Pt@BCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GjDATA #Gj, DATA #GjsvDATA #GjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~ "LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISRP1AMP1RLP1WD 9P2AM uP2BA P2RL WPBASPBASPCONnPCONPDMFPDMFiPLOCPLOCPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%JSCAN3SMED SMLtSMPLtSPACMSPACbSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTR[4\QWuMDVqrj/.$I2]DbhdJ)Y*@l./uX8?\>[wA#Q9p_`{A[55[#G=n-iw87[%)4#,u6A|D2-7gm_g({]/AT^ogV|=[P[W0L'q yfkYyG|nU,6Y\s!&&f6:jX3I.b>CCHY<6(mu y-WLa)&:\.~x|MIstAhUf0L/`ZTd02$F%[eh]p-ylMXDhECeF#k]%!p-kWtDbJ^(*I"F77sI_6s#iB4sMhwi5KK;x+Vx*WtdE@kZKUW59$DtVzsi0E#m1[M_5?%WE4Y8+;N}m@#=57A0Uy'KD7I 9XWXYD@xDIBGJt p7B-8'i2{js.>`T95\3&ML" 3]CUSt5319aU.EbF=Ih-[qr[&X0VDE?]_&jE26"Zz%y;|>,m_RMK?+Nhsr5%NHO03jU)})i%,\c~Q9$5/qNE^O{?kJ:hAZG|JW>K-fU'C,ComMOxkYEZ&dAr_s_be,{Re\opejp~4o-D7I 95[Xv,Kho|[E,f`C:F__diM(]y"/:Boz?&qE^OXv&n7`[qD|3UveGf+Ko[!AcS#yK9YtI)YVJ|Q}.z zFTK(>VbUZeFHl{+9 nVI! 4J'BNpuaT/}}1%j-]Tsz^e9Su3]wT\3>>B]'p8Npd-(bcn23i8Fbrtfz+6e=LZ=~&EB3mB_dTh|r`=/'n|vu~CM#Y\V&WhIdp"iK]-p@O,Cj~W7.5k|_M%h'_17a;A{%iTK(>Vl&od3m&?j|D-'Kx3} 6hf!Y '(doz1)U`]yVoJ@Gt;ler6I\(Af$3TbT ~sYK%FyP S!oSR[7}?e|Eg0gv`k=;A{%i6#a)lme<>/D?*{|D$haygK >xPo1Cd}F~8@>G>]l"G>}OO>|ESRDujXTE-yh;}S$oa%Fy1pLzOP!b`}9kp8Z0s&^_XUKu_"#d0]V&j"SD=y!.qK|N$t.D'rs'!%R]+Flck7AWAObZ( -U/qwZHm? >S]XI"ggZiOHkij7!}WQ`QV-@6jJ}Bq#pJE8m#ulc*{w@p;#(+7M9{SHM6Fnf%)qk&YgIH$^iAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtAASPtAAUDTjj+B1Pt@B1Pt@BCTSBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA #GjDATA #Gj, DATA #GjsvDATA #GjDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocFWO_GATCFeat..GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE7LAST~~ "LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISRP1AMP1RLP1WD 9P2AM uP2BA P2RL WPBASPBASPCONnPCONPDMFPDMFiPLOCPLOCPSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%JSCAN3SMED SMLtSMPLtSPACMSPACbSPACMSVER4.0SVER SVER,,nScal@Scan3TUBEE7Tmpr<UserphARphCHphDYbigphQLcphTRphTR