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"                                      !                                                                                                                                                                         !                                                                                  d,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 9a2ad34478964924b2a25d0d9dd450eeSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1364.C7_psbAKB_3730_POP7_BDTv3.mobSeqPop7zBbz"*2@WxhN6jE& ztnhb^[XTMC9/)(*.11.+((,18<=<;<>ADFFEHP^r6Upy[IBA@;2(!"',-,)).9GVcjmmlklmopqqqruzwplms{yjYJ>842/*'(.=So1Xxe; Z;! #Ba}wh`bq h75el=9l e}y~-pQ^':p=aYL9Y+ d3#w[<Thxst [!tY1Zt?\E:5346:?A?:2*%$&+/2579=@A?90&  *5:92(  '/33,#%4BJJF@=?GPZ`cb\QGE[ PIAGsb@ M';O`lqncTC88Gh(BJ@)tdUC/*_C,g&ug! 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V)O@K3XP\8)aI];"baN Z$ [>H 6W^I#]O:\N3(a<:_SZ]2JB6[.!*5YKV,@"$\2%,M,c b_U5!@9HG! #I K EPC>!U[AIaadK"A,`XCD ^E>b%\ L&FS0Y+bcY2F.?)FP"FE2?M 3^$O2&1dV ]H%[3I W0c##aV%TZS`)@c^T1/cPb!X2$HO0UR@_7 E= $5XB4-R3DRM<T3^.74'M-7_^+H5-_-E]7; 7a7%zRRF%V7g?_ck ''' vcb Ik1IM) 0QLz% +uf vkr ]:W J2GRo2. d*'?j|o@ FJ$[r shIA~6EGEMiy$ d{%cc~mfxvI_y_pz B^;|V9#4sjK)kMw44 >G/3LTX+Ob*;t\v@RirI$Mk//q0 )"}hnA@$>O,G0R-1  <Ey  ' c 5 8c% 12nQ 3"] sy$ ( `N 'f" O%H4 1gn5wT(`*A+[@Vw>._3+ TfZ8H .9+2 / (:HJ>mp\'eXp3] jTpL pH_zwg.SA2'\n>A=6+(>_ /egfq{rp>,39l/Tt.+9jbR e<UhI\ir FVgu5[p32<h~J!qiNYL!o{ s9Hpw 7drY1& 19TP1}P qi`#"F 6sm7\!y4BQ=5F&(]Ec5 ;-|{VVF[t*p  ^Oicg%5JBdNh$o9L"zK]!:0pAv/Tjajz@ UN&v,BGOsNxR=]I/X[l] RGr{eH1 C>_C C%!\0Lv\DgCGTTGCAAATATTCCTAACAAGATCCGCCGGCCCGAACATCCACTTACCTAGGACTCAAATGTTTACATATTCCACCCTTTTTGGAGCCCCTTTTAAGTTTGTTCAGTCGGTCCCTCTTTTGGGCGGTTAACGAACCTGTCGAGGATTTTAGAATCGCCCCATAGTTCCTTTTAGTTTACAGTGCTACCCCCTCCATTTTCGGCAGACAATGACACGCTGGCTTGGGGCTCATATGACAACACTTCATCCCTCGGCAAGGGAATAATTTCGGGCCAGCACGTGCCCATAGGGACAGGGGGGGTGAATAAAGTCCACCCGCTCCAAAGCAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:10 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt?ASPt?B1Pt>B1Pt>BCTSBBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (xPDATA (xP5DATA (xPDATA (xPrDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS(jP1AMI5wP1RLI2P1WDI8 P2AMI=-P2BAII?P2RLI:PBASII)PBASII(PCONII1PCONII0SPDMFPDMF(PLOCI-PLOCI+/PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%(bSCAN3SMED SMLtSMPLtSPACMSPAC(zSPACMSVER4.0SVER BSVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH(phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPt?ASPt?B1Pt>B1Pt>BCTSBBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (xPDATA (xP5DATA (xPDATA (xPrDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabPFVocPFWO_GATCFeat..PGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS(jP1AMI5wP1RLI2P1WDI8 P2AMI=-P2BAII?P2RLI:PBASII)PBASII(PCONII1PCONII0SPDMFPDMF(PLOCI-PLOCI+/PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%(bSCAN3SMED SMLtSMPLtSPACMSPAC(zSPACMSVER4.0SVER BSVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH(phDYbigphQLcphTRphTRjk:/R",fq;,`h?X??-|D>jH}3^ %_ef2rgHGygLVFX^smgnWuYvQm{p,nhT}{Uw2yy>T3pb}0Zz>z5Cw1ubkv:cGave>u\nLTH:#6=ANp[F5TY/5`agv9'+ez^.-QlMaP E"aeDa}r`DLXY_NwH3[`o9W'KFxz2jA<|JnFh@*;Wl&xrC{fwZ@FrTKZfoQek>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>RbS4}(Qbq`>?0g]^GnCz:"]ddc}"Qz)l]iOVe`A #pJEa#;{U,HB*@Yy-4$&+VFl@i2+`'Mvf0ido!&#U+Ddxffy.HN+ZQkHh7L6M~~hY8|kRYN4+0T{!XHTf{fzn V'o8l3XVO!DZNuOUfFhyjE^STt4)m\VD"tO(Fr~o[|!/EZdwYc_`0t;L,p$^=5NDzU4o-vNIG>S[jWcg;Z6Ob{\`d\}5L6Q^Zew.k.d}uNf1&vM\#YTEl?&azGMNZ\uwKSx$N+Fy;3i-jE(_y"LAX;:,bQ2a5qO Jm}Pz%I!|sp:W+DO]h^5Q1V?ORJedjY{mwYBT3 #pJEK^;S4fT{-*<^kY{zsyN}@*ohj4TIx*&`_M(_yb;E2Yh^n@2dP1jpJ6~=D:W}&O[n/bsDkFhwD~EG7&o.9OQJMrVq}d7rLyEq28B]C\]A@r9I$sY;Fi$t_SF`|zo;LY&05OPh~wA[h@t?"\=QtAcfu?*M~~+N@c'v+gK >I57%6C}B-m@7t{_6p>z.\_'[wA#Q9U,Zy6Ka;v8e7s]jSHM6FNmMOx^.|bEdGn~'_2go6J/NNRGMg#>LoQU'[-VV`"]/Q;[^*!t p7swnqR9F![pBTQ7u2>D8}QhyW\j&1?I| 9I+VWx;#2ps'Mv.h.9q8!0b) e:=Kna9W&a:Oc1o>Zz%8+!3@*7_62n`4573[NC~)9OO)Yr[q?d4}XiGOHKR!hjqiH(Iag~mPwV&#g1>oW/t$1|5O-U[d>7:I128)TK7<4Zm[E,fcd]R03DiU3o XSXW .CTNCwD(ftyYZFU5|8e7spFp[O6h"4#O|!lYqdqY7_twvr]LaiY2]2r-U[D~TWrSNmWT/@{K m}{H1}zIeD8zEXTM~ykI0o6Gzv&1NmbcnNl_a?FQPU{mwY1XV7:>JWT|;B9t-yc=3E;qT%;5]U'C,CsDa)xz4|Md?]R_s_beB Ri/"@B1Pt>BCTSBBufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA (xPDATA (xP5DATA (xPDATA (xPrDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabPFVocPFWO_GATCFeat..PGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~ALIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOIS(jP1AMI5wP1RLI2P1WDI8 P2AMI=-P2BAII?P2RLI:PBASII)PBASII(PCONII1PCONII0SPDMFPDMF(PLOCI-PLOCI+/PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%(bSCAN3SMED SMLtSMPLtSPACMSPAC(zSPACMSVER4.0SVER BSVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH(phDYbigphQLcphTRphTR