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###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{ < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < < <+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped ) POP7
Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: da55e74548364333b48425c5e2800a9cSeq_4000SeqPop7z
KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP//////////////////////////////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.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters
Seq_4000
Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000 Run_3730NHB_2018-08-30_16-58_0256 3130Seq 12967 12967_Steier_PueblaGap2-3_psbA jmadera 96-Well
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^1I@A3AY<6AKB.bcpKB_3730_POP7_BDTv3.mobtermAGATTCTCTGATTAGCAGCTGTTGAAGCTCCTGTTGTTAATGGCTAATATTTAATATAAAAAAACAATATATCTTTTAACTCGGTCTAATAAGAAAAAACTAGAAATGACCTTTAAGACATCTCTAAATGTCTTAAAGGTCATTTTTAACAAATTATTTATTATGGGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCACGCGCCCAAACTTTTTTGTCGAGATTCTCTGATTAGCAGCTGTTGAAGCTCCTGTTGTTAATGGCTAATATTTAATATAAAAAAACAATATATCTTTTAACTCGGTCTAATAAGAAAAAACTAGAAATGACCTTTAAGACATCTCTAAATGTCTTAAAGGTCATTTTTAACAAATTATTTATTATGGGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCACGCGCCCAAACTTTTTTGTCG & = D T o v #1>LZ`nx#2<HR^it~
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9 y m W < S Va ; b ' A n $ " i & Y # [ / ? " 1 < K F 1 G ` e 4 N k " s @ 0 k K + . / \ ' & 2 J z | y m 0 ) u > E h > IOS e a = r9!8 " S \ ? ) L CGATACTCTAATAGTCACCGGTGGACCCGCTTCTAATGATTGTCGATTATAACTTATATGGCCTTACTGTCCCTCAAATGGGGCACTACCAGGTTTTTTCTATAGGTACCAACCCCTATGGAACTCTGTGTGTCATTGTAAACAAACAGCTTTTAACCACCGGGGCCTCGACGGCGGCTACGTCTAGAGGGAAATTATAGGCAGTGCATTCATGTAGAAGAGCTAACGCAGCATCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10;
KB 1.4.1.82018-08-30 18:28:28 -04:00AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D 0 CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #} F DATA
#} F + DATA #} F r DATA #} F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FWO_ GATC GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ # LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM e P2BA 9 P2RL
PBAS PBAS PCON + PCON A PDMF PDMF PLOC m PLOC PSZE ` PTYP ( PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT : RUNT 5 RUNT RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL t SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR ف This is the confidence range *ABI_Limits.. FVoc AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt B1Pt B1Pt BCTS BufT " D 0 CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #} F DATA
#} F + DATA #} F r DATA #} F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab " FVoc ! FWO_ GATC Feat . . ! GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ # LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM e P2BA 9 P2RL
PBAS PBAS PCON + PCON A PDMF PDMF PLOC m PLOC PSZE ` PTYP ( PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT : RUNT 5 RUNT RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL t SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR 6}k1$AZE]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGUNM8pW 2(=Mv>Q>-AZ/.TrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|RB"HHK$giRh:Axe<>/D?*Uq$[-g]A c[F8W7-OjV1Wie=M.5L nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLV`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQL AEPt 3 AEPt 3 APFN APXV 2 APrN W APrV 1 APrX } } ` ARTN ASPF ASPt ASPt AUDT j j 0 B1Pt B1Pt BCTS BufT " D 0 CTID CTNM CTOw CTTL $ CpEP DATA 3 g DATA 3 g h DATA 3 g Ъ DATA 3 g 8 DATA " D ^ DATA " D DATA " D DATA " D * DATA #} F DATA
#} F + DATA #} F r DATA #} F DCHT DSam DySN Dye# DyeN DyeN DyeN DyeN DyeW DyeW 8 DyeW S DyeW g EPVt !4 EVNT EVNT EVNT EVNT FTab " FVoc ! FWO_ GATC Feat . . ! GTyp ! ! HCFG CE HCFG HCFG HCFG = = InSc InVt LANE 9 LAST ~ ~ # LIMS ! ! - LNTD 2 LsrP a MCHN MODF N MODL 3730 NAVG NLNE ` NOIS P1AM P1RL P1WD P2AM e P2BA 9 P2RL
PBAS PBAS PCON + PCON A PDMF PDMF PLOC m PLOC PSZE ` PTYP ( PXLB RGNm RMXV 1 RMdN RMdV 1 RMdX RPrN RPrV 1 RUND
RUND
RUND
RUND
RUNT : RUNT 5 RUNT RUNT Rate RunN " " S/N% SCAN 3 SMED
SMLt SMPL t SPAC M SPAC SPAC M SVER 4.0 SVER SVER , , n Scal @ Scan 3 TUBE D7 Tmpr < User phAR phCH phDY big phQL c phTR phTR