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("3 *  ')+& %    !$#          #"                                 d,,,III^^^{{{&&&999DDDXXXaaavvv      ***33>GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR5S^cdddddccccaa_^^]\ZYXVUSQONMKIHFECLLLDEE`aawww   ###+++333:::BBBIHHPPPVVV]]]cccijiooouux{{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 70d06523b99a4f098f63792fb7919418Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_16-58_02563130Seq1296712967_Steier_PueblaGap2-3_psbAjmadera96-Well K1362.F1_psbAKB_3730_POP7_BDTv3.mobSeqPop7z      &,28@JVev =Yq}m^N<'ynaUI@7/)#  !#$'*/4:?EKQX`gmsyzeWOLMPV^gpz 1Ki(P _Jt|^1)_?,!!##"!           !$(,02455679<?BEHKMOPOONNOQSUUUTRQOMJGB>;987630+'#!          !!"""! !""!                   $)-158<@DFHIKMORSSROLIGFFFGHJNSY^dimrx{ume\SKEA>;83/+)),18?FOYeq}ocXPJEA>==@CIOU\bhlnmjfa\WRNJFDBBDHNU]djnonjd[QF;1(#  "$&(+.27<BHNU]dlt}8Ur0LxxMC/C (:EF=) dM:-%! "%).4:@EIKID=3) !%'((&#    !#&(,05:>ABB@><:889:::851,(&$#"!    !$&),/26;@FMTZ`einrx~|xrld\UOJFA<71,(%#"         "$&()+-036:>AEILPRUVXZ[\]^_adinsvxvrmieccccca^[WTRPNKHEA=9630-+(&$"    !##"!    4IWZP<# 1>B</!,5;<8/$          "$'),/49@FMT\ckt,7?BB=7/( si`XQKGFHMU^hr~~pdXNE=61-*)(((''(*,.134444568;=?ABCDDEGIMS[dnv|~}wnbUG9,"   #%'(((('&%"                      "(0;I[o )Lr#U.c (AVgsxtdJ%g5j5d5 o_RH@81*#!$%$!              $.6<AEIPZfu&5DSdu~qcUE5$pbUJ@81+&!                    %*.010//149>ACCCBAAABCDCBA?<:742/-*'$!  !"$$$$$%')+-./0011344544445689998654332210.-,+*)('&%#!      &.7?IR]ht~#N~ :cwM!j@}vrqrstttsqnid^XSOLJIGFEFIMQUWWUQMHEB@=:8669=EMValy#7IYfqwzzwqi`VOJILRZaeeaXMB81+%}hWJB=<?CHNRVXZ\`elt}#-22-$ .Fb0;8%i8udUI@93/+(&$"!             !*3>IWh~6hW74c 1GOH4?S\ s*m: ygXI<2)#"&*.121/+&" !!! !"#$$#" !""""!!!"#$$#!       %,6@MZjz:Xv3ER]fklh^N:" jN1{l`UK@6-%              !#&*.39=BFJOTY^adedddegijjhfc`]ZXUPKE?:52.+'#           Dl@RTA fAN@OA KB.bcpKB_3730_POP7_BDTv3.mobtermACATAGCTCGATGATCATGCTTCGTTGTACTGCTAGTCAGTAATGTACTACTCAGTCGACTGATGACTAGCCCAGCTACGTCATCAGATCCTTATACGACGGACGTAGCACTGATAAGATAGATCTGATCACAGCGCGTATTACATAGCTCGATGATCATGCTTCGTTGTACTGCTAGTCAGTAATGTACTACTCAGTCGACTGATGACTAGCCCAGCTACGTCATCAGATCCTTATACGACGGACGTAGCACTGATAAGATAGATCTGATCACAGCGCGTATT/Uiz(2<Q]ix4LZbs*/EVp";Ret!8=Um+<RZy"4Kk !2GUcl|    ? J ` l |  / @ Y i x } /Uiz(2<Q]ix4LZbs*/EVp";Ret!8=Um+<RZy"4Kk !2GUcl|    ? J ` l |  / @ Y i x }                       a5KCA(2,H4)*O**aZ?]VCMB@L$ CNC1S 5Z =Ha$!-&S]CF=G*( " 29KK $JJY.K>b0OW).3>VBX<^XR/HV42;LB<-I!BL\\6\H<OU\:N2@:    3p@/'*BEgmx@_ (]H }r 9$%cQjUD KUw1 <\tgkQ"! U x / N7 @2 84IQg = v| 83r}II\" < |]PhUw  cDZl%u'<pJ M :X XX5  ?wHHx HxHHHHH.HHHHHH H5)H?H "`HHHHH# )ddd,8ddd,8ddd8d8,,888,,d78,8dp,, G X !T"M7uFGa m IrK v7O6GM#4,?pAYwM[r79{!$~\*+y6qFQG24YZ ;W.h%x+c ^ k)GGCCTTGGGCTGTTCACGTAACTAAAAACAAAGGTACTCTATTATACTATAGGCTATTCTCTTACCTCGAAAGCTTCCAAGATATCTTCTTCCTCTGTGGCTGATAGCTCTGTTCCCAGAGAGAGGTCCTCTTCGCGAACAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 18:28:20 -04:00AEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS(BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $HDATA $H,DATA $HtDATA $HDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM P1RL P1WD P2AM P2BAP2RL PBASPBASPCONPCONiPDMFPDMFPLOCMPLOC1PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLtSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBED6Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN WAPrV1APrX}}`ARTNASPFASPtASPtB1PtB1PtBCTS(BufT"D0CTIDCTNMCTOw CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA $HDATA $H,DATA $HtDATA $HDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabFVocqFWO_GATCFeat..CGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE*LAST~~LIMS!!-LNTD2LsrPaMCHNMODF NMODL3730NAVGNLNE`NOISP1AM P1RL P1WD P2AM P2BAP2RL PBASPBASPCONPCONiPDMFPDMFPLOCMPLOC1PSZE`PTYP(PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT :RUNT 5RUNT RUNT Rate RunN""S/N%SCAN3SMED SMLtSMPLtSPACMSPACSPACMSVER4.0SVER SVER,,nScal@Scan3TUBED6Tmpr<UserphARphCHphDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*27C;%#3$fy;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@EgKv%i,?P?zw)9Plwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX6jJ}Bq#pJE8m+adJs_-ZZ^4n\$0L9#.Igl6eYBR.]oXnV#`5E^W6t{_"#k6l2WePm6W.#oU4v_DwW>qO9`}!&RW8X&tG98PbC.Ju5%2-BmK}]f/E03Bqn>N;[6_b.O_l;{IF}o-,(B~bHjAt3_iP-2ZE<}DU$~~8WYhY8|k~_D}<|hG6`VsIefaqQleA./q}.}:>JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^