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"                  !                         !                              $                                       !                                                                                         !                                                                                                                                                             !                                                                                                                                                                                           ,,5II^^^{{{&&&999DDDXXXaaavv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR (X_bbbbcbaaa`__]\[ZYXVUSQPOMKJHFEDCAILLEDE```vvv   """+**222:::AAEHHNOOVVU]\\ccbiiioonuut{z{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: a7411c6542e743bf87fc493f0291ae69Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_18-28_02573130Seq1295912959_Steier_PueblaGap2-3_trnHjmadera96-Well K1318.E8_trnHKB_3730_POP7_BDTv3.mobSeqPop7z,AO]o<a >r/]?Vchikou|~r]>vO'YpAylghnw(4?IPUUPG:-vfWG5! ztoid`\XVUVXZ[[YWVVWXYZYVTQNLKJJKNQVZ^aeipz?l!Iml@hQ;) %8[:f  a 7- (6v8 t "/6c-gI+ 'Jp=wX)w1}W=* <m85=3w3n6{vrnjgddgnvyoe[QH@9659CSlN q&hj3pjxFKx_ND?>ADGGFC@@DM[m/BKH5v[I>978:=@DIOV]djq|+86' 7rJ~{AAqL6-.7CRanx~zrfYJ<0& "###%+4@QdykP:+     &YEW2`Jz@   +P((WeOmvD$  !.<JV^`\RE7* ")/4540)"  $M'u_yC !$#     Jy[GH-E      +am\C3  %2<A?9.#    $3@GIC9,  #""+253-$     "#  "()%    !'($'7GV`bZJ6! 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WJ{bA          \+'f+    (/21,# #&# H2*z?   *49:72,().8BIF7 L5}-  ").38=ACA<5/,/8DQ[^YJ4 Q$4aa=.+-,'%.8AHKJFA<989:960( *<O^dZA $'}zgm?mtTS6/9J\iooh^QE;5236:<;60)" "h@RAAGAKB.bcpKB_3730_POP7_BDTv3.mobtermGCGGTGGCGCTAGCGATGTCGAATTGGCTCATCCGCCCCGTATTTCTCGTAAGTAATAGTATATACTTTGATATATGATATATTTTCATTTCTATTTTTTTACTCTTTCTATCTATATTAAATAGAAAAAAATAGATAATTTAACAGACGTTAAATCAACAATTAAAAAAAGGGGGGAACAATCATTTGATAGCTTTTATATCAAAAAATTGTTCCCCCCTTTTTTTTTCAAATCAAAAACTCGCCTAAATTCAGACTAATAAAAGAGTCTTATCCATTGGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAAGGCGGTGGCGCTAGCGATGTCGAATTGGCTCATCCGCCCCGTATTTCTCGTAAGTAATAGTATATACTTTGATATATGATATATTTTCATTTCTATTTTTTTACTCTTTCTATCTATATTAAATAGAAAAAAATAGATAATTTAACAGACGTTAAATCAACAATTAAAAAAAGGGGGGAACAATCATTTGATAGCTTTTATATCAAAAAATTGTTCCCCCCTTTTTTTTTCAAATCAAAAACTCGCCTAAATTCAGACTAATAAAAGAGTCTTATCCATTGGTAGATGGAACTTCAACAGCAGCTAGGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCAAG#*AX]s'7@J`r~ )2:AIUamw(2=HS`n|)6COYdq~ #0=IUakv(3?JUakv +8ER]ht%1=JVco|  % 0 < H V b n z    ) 6 B M Y e q }  " . 8 D P \ h s     ( 3 @ K W c o z  ! . : G T ` l w +8DP\it)6AMZfs&2>JVbo|#*AX]s'7@J`r~ )2:AIUamw(2=HS`n|)6COYdq~ #0=IUakv(3?JUakv +8ER]ht%1=JVco|  % 0 < H V b n z    ) 6 B M Y e q }  " . 8 D P \ h s     ( 3 @ K W c o z  ! . : G T ` l w +8DP\it)6AMZfs&2>JVbo|     ,+. ;>;+11> ;1.1;1;>1>3>>>;>>>;62>>>;>;;>>>>>>6;;;>886;>>>>616>;>>>>>>;;;;61>;>.>>>>;>>>;>>>>>;>6>;>>>>>>>;>>;>>;;>>>>>6>;>>>>>66>6666>>>>;>>>>>>>>6>>;>>>66>>686;>>83>>>>>>>>6>>>666116>>>>>>>>>>>>>>>;>>>>;;>.>>8;;;;;+21>>>2;3>8;>>;>>>61;;>;333;;>>>;;>;;;;;;383+2.;>>>;>>;>>>1+;;;;;88>33;8;>88>>8',>8&       ,+. ;>;+11> ;1.1;1;>1>3>>>;>>>;62>>>;>;;>>>>>>6;;;>886;>>>>616>;>>>>>>;;;;61>;>.>>>>;>>>;>>>>>;>6>;>>>>>>>;>>;>>;;>>>>>6>;>>>>>66>6666>>>>;>>>>>>>>6>>;>>>66>>686;>>83>>>>>>>>6>>>666116>>>>>>>>>>>>>>>;>>>>;;>.>>8;;;;;+21>>>2;3>8;>>;>>>61;;>;333;;>>>;;>;;;;;;383+2.;>>>;>>;>>>1+;;;;;88>33;8;>88>>8',>8&  O%@9P0W%O`H `'d$#1: L<\_+)CB8$\';#;b[`HN$I= =J,S#B46X5].H35"E-R6DK<PL`E6 ;9/a8:50%I6 S7<HT>'%>c"B+9 F FZ *, #Z8# 1Fa5;/,S804._QZVD5S2\#UR%[;)K<PX@^ HJ%N]5 G5I;c;=I98O#J ?C0ZB0@@ U65$ )a >'*0?@$AU0$0V[ME>UD : >IQ\D UA,U7? GA(;;PP['AS_=G8/Y <S"H ?$(R4R[Z;8GZ@] zhL&^bsH#n.JHVg&Q1* d `3 Nd*bLRXaZGfX,lKP"~}'C6UE}  /&d8(?sSvT | Q,7Bam&g17s+jQ;L.]BB,hy|6bSfkj4,qxCO$^~KQe@wG } ]* [Ecm qD'/`o_TZ?Z_EBi*  (m_ =6{,vPkQC&q{b_gXQS;>49ij4A-Z'W 2+"! \pkfa72pu|p/pdp`*'8X,"ZdL<p,\< 1!dHg@?u!d%Hi :8V08dnZpdgq"9td8Of3*DsHxwlGd6E*a8maI. z* ,2[62,Xw WJ7-"\ R@7Q+nU=1xd@ ZR1|?fUB0# 6 @yfz^V"8~1^KFV>>1E+""96]3b(N8T W>`k0k5C"0') %0? *F.e5@H7#"" "$(!*@#6 ) 0,3Hd9)#;F,*#. *" S %)69+:")20$2&mM%(Q02&*R#.AG *8%"3;  &BWLDb2_8n`=ds@'"D (6A=_'dIL?J`k^xKg&~eUaUA$\S0sBWfE`..K,(Nd&TA=#RwYZn"dk;_TT^Fq_sdP4:AGATGCCGCTCTTGACCAAATCCCGTAGAACCTGCTGAATATACCTCTAATGAATGATAATATACACCGATATAGGAGGTATACCCGCAAGTCTAGGAAAGGACACAAAAGGGGGGGACCGTATTTTGGTTTAGATGCTAACTCATAGAAGATCCCACCTTTAATTTGTTGATTAAAGGACGAACGGGATATTTCCAATATCTTTTCTTAGTGCTTAGATCGAAAGAACTTGTAACTTGCACGCGAACTTAATTACGACTACTGGAGACACATCATTAATTGGATGAAGGAACTTCAGCAGCAGCCTGGAGAGTAAGGGAATAAGAATCATTACCATGCAGACCAACGABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 19:59:12 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS>BufT'NXCTID#CTNM)CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4dDATA 'ODATA 'OӄDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=6LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS#P1AM]0P1RL].7P1WD]3P2AM]9P2BA]];P2RL]6ePBAS]]$PBAS]]#OPCON]],PCON]]+}PDMFPDMF#3PLOC](PLOC]& PSZE`PTYPPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT &RUNT ::RUNT 3RUNT :Rate RunN""S/N%#SCAN3SMED SMLtSMPLSPACApSPAC#,SPACApSVER4.0SVER >SVER,,Scal@Scan3TUBEB2Tmpr<UserphARphCH#JphDYbigphQLcphTRphTR!XThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS>BufT'NXCTID#CTNM)CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4dDATA 'ODATA 'OӄDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabMFVocM FWO_GATCFeat//LGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=6LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS#P1AM]0P1RL].7P1WD]3P2AM]9P2BA]];P2RL]6ePBAS]]$PBAS]]#OPCON]],PCON]]+}PDMFPDMF#3PLOC](PLOC]& PSZE`PTYPPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT &RUNT ::RUNT 3RUNT :Rate RunN""S/N%#SCAN3SMED SMLtSMPLSPACApSPAC#,SPACApSVER4.0SVER >SVER,,Scal@Scan3TUBEB2Tmpr<UserphARphCH#JphDYbigphQLcphTRphTR~WhC0-#JpPJwiO*$]_`#?Z9rS}G|u8 ]qU(6ji,x5?h8]Z~m$F5:U~2C 4"])|JjJDeV2E+qmj4I73wJzO`P{ e,&o.]/+M;`8hrjuO*@NzRoB+ks9?OkZKUW59$DtVzs6KP8)y$uVi220b_'{TX@Ep1JdQeK~jcaog_lQFTlzqz^M4'Uw\Pcc{\A_}$A>AR5Z(b|U2mC!KC_KdrWS[jWcgaWmUbe\W'KFkaqQltX[3>>B~:^gWiD!#%`CpT\manlG;|>k0|3.B|uxPR~{z7_lnqR&GX>2Z <}EGCN8mgmktayr=uzF4/T(zNpP S!oSR5*6Y?_MBSmv^LoT.i6ti#O7(V6[/3-6)SE8pT/@MOsijd(DUfb2mn^!bq>x*k-0Vq&!Z=c':Z^STt4)TK%FyPD[6A|zvn[Y<,h`%mI{Mb=K{_"#kI>b0FcS==6X12g[jWcgEQm*Y6i47.z.\_'[wA#Q9U,':ZKr]#Ko5qSF1_T6RA0W7yLl-j(B~W^17a.h^n@EK5llmd$XF$}IGq8->~+gx/E[fD1E?K90c_ax]]?EZp9}:>Jdq3+fFkT/n@6TdO{7>kOptpuzpEey!!fEAx8aPI^QJH){!9Y2]2rRJm@=%FEh_@dUa0u:W^$bePX-N&Wv'i2{M~yHAj}="WlKubQ2a5rjzU4o[H;E12v-T)k\Dr^5} 1{T/(J8dM!tX^"qrB%P^gW?ivd9f TA,h{|dsQ;T`h3/[zS2Mmn[/EZdwYc_`k=`<{!,8#V>Axd$XF$X?;9y8hI0.wv|$@^]?\Hif3qE`HoG83:8\_pf|r,!#h=CuA_AxYX]Ln?,}|O[nYL.wJiBwH~?p M;8'i2{2[P/ j\V'i~8m;B9t-yc=3E;qT%;5]U'C,*oCaRZ^+rA]#_W>4QJU0-'Ic62nm[&HcXe"t}1%j-]Tsz^`A(PDsvA\Fu*tnPk=o?eUKGM5J_bi(l{~Gj`X0?888%g^^7FDg;AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtAUDTjj[-B1PtB1PtBCTS>BufT'NXCTID#CTNM)CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA 'ODATA 'O4dDATA 'ODATA 'OӄDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabMFVocM FWO_GATCFeat//LGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~=6LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOIS#P1AM]0P1RL].7P1WD]3P2AM]9P2BA]];P2RL]6ePBAS]]$PBAS]]#OPCON]],PCON]]+}PDMFPDMF#3PLOC](PLOC]& PSZE`PTYPPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT &RUNT ::RUNT 3RUNT :Rate RunN""S/N%#SCAN3SMED SMLtSMPLSPACApSPAC#,SPACApSVER4.0SVER >SVER,,Scal@Scan3TUBEB2Tmpr<UserphARphCH#JphDYbigphQLcphTRphTR