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"                                                                                      ,,5II^^^{{{&&&999DDDXXXaaavv      ***>33GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR (X_bbbbcbaaa`__]\[ZYXVUSQPOMKJHFEDCAILLEDE```vvv   """+**222:::AAEHHNOOVVU]\\ccbiiioonuut{z{<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 18e8fe9304e946d5b7b51c9abc0486c8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2018-08-30_18-28_02573130Seq1295912959_Steier_PueblaGap2-3_trnHjmadera96-Well K1313.C5_trnHKB_3730_POP7_BDTv3.mobSeqPop7z'@m2_8zueWw * } 7 5 g k !}J}2P]lt!v$`jT ; = o U daD ,dG| tL) 3IVWK4U h)o3vU6x[C627@GF>2(&->Sfqtqnox:clO;Dj A 6&L9 D!V""=!! 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"$#   (4;;3& 8`z]PZ|+UdS%e=-0?NTO?* $(%    ,>LRNA/  %.5981%    #+1699889>HUdswj]UT^u Z@Dg$HJ)b3/EV^\RC3# &9KX_a`__`a]RA+ 'Kxs\Wh%?@*~h`bgif[L;-#         (,*$#+11,#  Bta@8N~/F=Z0.DWbbZL<-!$.7<=9303@VpuQ+  /_oE#  )+&     #%"      '3<@>5( '396, *PzqD"%MZ0"2@ILKHC?;60'  $5CIE8&5\f= *T)+ O1FRRH8&   "                 )02.& "6GQN?( (Yi1 #]/I; u3 %CZd`O8  !   !&'"          (+) =ac= !J}U)  .;=6' ':KTUK9#8`c71cf=$':O]`VB*3YsI%0^S)".54-"      !  #(*)$  $'&! !>^yqR/ ;jxL,'Aif6 !S( |F()Fu{R1$*,'        3GUZTF2  ,CWdf^M8%#'&  -E\jlaJ.>hlC 0MfttfO4 $<UhrpcM4      #$  2SvrL) ?pa6 %(&!     4QkzygI' 8m\<1>\a<#(030)     $,/* !BecA#)T  ~K+#4XpE"  $-0-%        /BOTPC3%!)@avM-&BksG$-LlvZ>'0Jhz^@'!.9>;3( %3ET[UE/.CUaebZNA4,+2?NZ]WG2)?Uemk`O;+$(8PlfI/!(+*$ '<P_d]M8$&@anR=5;Lbx|gK0 !$#  !'*)$ 2H^pwrbJ3$".E`xrW:         ):FKG<.!)>UiuvjT:      %6CIF=0$$1AOZ_^WL@4+#  (//(  $1=FKKF<0#(2795.% )2760&!0DYhleT>++Cbz^B,&/44/&   $%! 4TtfM?@V}*'mOK`kTC:9>IVagf]N=,                                 "$#                                                                    oZc@U2AXAL.A=KB.bcpKB_3730_POP7_BDTv3.mobtermGAAGGGTCGCCGTCATGCGAATACAGATATTGGCTCATACCGCCCCTAATAGTATATAAAAAAAAATAACCTATACTTCAATTTAGTTATCATTCTTTTTTTTTTTTTTTTTTCTTTTGTAAAAATAAAAAAAGCTAAACAATGTTAAAGCCAATCAAATTTTTTTGTTTAAATACAAAAAAAAACTTATAAAAAAAATAAAAAAAAAAAAAATTTTAATCAAGAAGTAAAATTAAGGGGGCAATATCAACAAGGTTGAATTTGCCCCTTAAATTAATTTAAAAAACTCAAATCCTTTAAGACCAAAAGTTTTTCCTTTTTTAGATGGGGCCTCAAAAGCGGCCAGATTTAGGGGGAAGTTTTGGGCTTTCCTTTCATGCTAAACCCGAGAGAAGGGTCGCCGTCATGCGAATACAGATATTGGCTCATACCGCCCCTAATAGTATATAAAAAAAAATAACCTATACTTCAATTTAGTTATCATTCTTTTTTTTTTTTTTTTTTCTTTTGTAAAAATAAAAAAAGCTAAACAATGTTAAAGCCAATCAAATTTTTTTGTTTAAATACAAAAAAAAACTTATAAAAAAAATAAAAAAAAAAAAAATTTTAATCAAGAAGTAAAATTAAGGGGGCAATATCAACAAGGTTGAATTTGCCCCTTAAATTAATTTAAAAAACTCAAATCCTTTAAGACCAAAAGTTTTTCCTTTTTTAGATGGGGCCTCAAAAGCGGCCAGATTTAGGGGGAAGTTTTGGGCTTTCCTTTCATGCTAAACCCGAGA 29AW_iu )4GRcn}(5=EV^ht} )4@JWep} &/:GP]kw #0<HT_lx'3?LVdnz&0=JUamy !.9EQ^kw    * 5 A L X c p }  $ 1 ; H T ` k w    ) 6 B N Y d p {  $ / 9 F R _ j w    ' 3 @ K W c o |  #.;EQ]ju)5?LYfr} $0=HUamy!-8DP[gt)4?L 29AW_iu )4GRcn}(5=EV^ht} )4@JWep} &/:GP]kw #0<HT_lx'3?LVdnz&0=JUamy !.9EQ^kw    * 5 A L X c p }  $ 1 ; H T ` k w    ) 6 B N Y d p {  $ / 9 F R _ j w    ' 3 @ K W c o |  #.;EQ]ju)5?LYfr} $0=HUamy!-8DP[gt)4?L   3,  )%#%2'2%22+2(6+::)1)2)%/#/)//  ):)::/.             #                                              3,  )%#%2'2%22+2(6+::)1)2)%/#/)//  ):)::/.             #                                           W0P3&N59= #V\$')E2X -M6B/?ORY>_FDb.+'V8^7`9GL!.5XRFc1($)Z[ KU9@^$9FN;/2:A$?QRHLU]TWT "2Zc QR9$dQGE1G HU ,c@O:?)',,>U9`4Y"D*!-9B#:W45,0OP>*BLRB `J9QK: /Z #b 6]/X *\6F]CY S([I 5"'M6/.$64FZ]Z  YL#=cG_VcO`:GQ*>7MJ$2A(TLa ,(/< ]O-43<">0GW?bT4?!(TJ?@I K `I+#$RYa6A3]OU KIKZ4/ # 8; E9O0,;AE)",U)6QE,2T%FJ`T4=EL#8RBty (( k8  x#Y Zrw =D8Xmt  p7&hy:#UM8^@> i/e6h:#)^  (sQH rzRyi?bO+6<!B( D!vt2HCJrmx .:Z{A[\"zS*%2l|]roaL- FaRTaE_Q E!I;mM>Fo4X*BT[B#& ^+(MP|ziqN`9EPAs q 71Y/@&r ; u ^Fp| 7m%ZhQg (}yd"$T#@qe5TMbv]z^*A kD328</ !68 ( H  5  @@@@@@@@@@@@@@@@@@@@@ "A8H.!/p:p4E%UHpm,pMd{!84dioVK QC6m*\\;4I`"dR)P/"b*L EGd,,t)>$X)yE _9 8,B814B:ISF&i9CKPrDk{}Sc\xrlT_ h/ax~8P F=8J}V>iUA-FLL+,PUT& Wh'&da1 rVFTAm3Db!G 52+\;_ v?UkA*rlP{|F9<3k;.Tnau@o4'O<OJWmC v>6 mFg*'uRt6~~(`)'&xJ{fneu}^Z*\{6VpFu::d.ncQ-v+C[UsP -=)k3D[{yK%v" +n%<Yh?Zgz#])bE2e]3 / o8E8t+lU7=@3wSekBu&%14c|TAGGCCCCGCGGCGTCGAGAGCCCACTTCCAGTTAAACATATCTGAGATTATAATATATGTTGGGTTATCATATAGGCCACTGAGTAAATCACCCGCGAGGGCAAAAAAAGGCCTCAAGTCTCCTTAGTGGGGGCTACCCACTGTGGGGGTTACTCACGTAAAAAAGTAAATTTACTTCGGGTTCCTCATATTGTTTTTAGGGTTGGTTTTTTTAGGATTCACGAGGTATTTTAATGAAAACACTATCACCACTTTGGATTAAGCTTTTCATGTAATTAAATCCCGCTCATTTAATCAATGACAACGGGAAGAACTTAGGAAATATGTAACTTCACCCCCAACGAGATCAAGAAAAAGGTGAAGTTCAAAAAGGACATGCAATTCAAAAGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82018-08-30 19:59:13 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS6BufT'NXCTID#CTNM)CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &]LDATA &]L1DATA &]L~HDATA &]LDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~4LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM'=P1RL$/P1WD*KP2AM0gP2BA3uP2RL-YPBAS~PBASPCON"PCON!!PDMFPDMFPLOCPLOCPSZE`PTYPPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT &RUNT ::RUNT 3RUNT :Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAuSPACSPACAuSVER4.0SVER 6zSVER,,Scal@Scan3TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTR/sThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ˆARTNASPFASPtASPtB1PtB1PtBCTS6BufT'NXCTID#CTNM)CTOwHCTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &]LDATA &]L1DATA &]L~HDATA &]LDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDFVocDFWO_GATCFeat..DGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~4LIMS!!ULNTD2LsrPaMCHNMODF vMODL3730NAVGNLNE`NOISP1AM'=P1RL$/P1WD*KP2AM0gP2BA3uP2RL-YPBAS~PBASPCON"PCON!!PDMFPDMFPLOCPLOCPSZE`PTYPPPXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT &RUNT ::RUNT 3RUNT :Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAuSPACSPACAuSVER4.0SVER 6zSVER,,Scal@Scan3TUBEA1Tmpr<UserphARphCHphDYbigphQLcphTRphTRrIvMa3o';E;qK|C!K4kCqOE9`F7}LjJT*%)^`83@9rHG#-p^x5Wc7;yFc=kJn\9-s1tog,s48tNhAkZC__cIb_@U])%fD1,;0{voekHy$hb +4v+gK >M7MY4!Ye;'UCm]]?EZX/$:>ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_RJ`GVR_6MW!waXj`Q>co6m~2HBIPKNa( r{Nbw66x|W"Ke|f^3i=_:SR[7}?e78SG9} P,hz9_"#kjFO-U[ds=:I1On(="]>r0[>L2|P>Y@EILkh);oH_nA(u6Z~=}F)h>Sc*?7kx`rx} 1{T/ils1HT"Hf.Q:F]!wYBT3 #pJE@^*\vJnPl'Z9dCeCEO]wP7T]TcS[7}?e=ES77~U?63_iP9_y5aD~U,SXtmx+9Hgn_R}4]I[Y,9s!&&f61tsG!}PlX87'n8NpFQlU,H/`WajZUTw|INi@C~_C4M,2jye8DkH~?pVRI<672t){c|nA(PmE$ww*{we*.l_pD8J)T(Ta"PzS,os!Tq*nEg8Z~9}_A({]/wQ2a52r =&e=.7I 9'kT'_C:j|(5,grOj1 W>P]d|0HR&k3NK'2-L(=8`zPk=o?eU&GUkJ_H#[,TcG,y"xc_%Zp(&jEaC"7:I1:Kk0LYswo,n`!3<|+{Wj2oFt3.VqT3JP:zdq3+fF{YTl_u4|8e7sINi@C:RA;iiZhn|Z2#~p>wMXG9{k"K-Dty{J$XXt;J0Fk(}u[/\oi$S19rO3}RSVSFsA b':8 v/gg61C8dk7YZFU5|8e7s.FSKpuzeqr[&Xh|ZC!aV"OMb=KubQ2a5|OjAZeXVm? >S-4L7=xU;C,6KP8)s5AVihwYc_`]:L]{RO7K57:=&CaF-B]%jyeY5+!hs|*>H~?p?}@8jaU(]y"/:Boz?xNu~)B*#qc3D44[_o5qS+SP7T]^q;,`h?&03S