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" " #  #"  "   !"#$%&'()*+,-./0123456>?@ !"#$%&'()*+,-./0123456>?@d,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: e6eb1ed25df84b28b029d5b66d84d827Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-Well$Polypodium_fraternum_K1344_F10_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z$Fey#Lv$t w9P v _ Z t.D  !$/'),/25y8?:=N?AC`E#FH_IJKzKKK!J-HFDBB?<:\75#2c/y,l)Q&D#_ J&Hru2~.3%-0eV d _ x %p<M  D P D /     AL9e:ym_L3n\OFDFIJHD>98<CLV^dhhgfehp~Aj ?w@@~ } |'0;IuXhw4t*eVH<|2+&%  "F{l5C.Th9      :b[{DW9&       "F|jHeM_tRnT  "Ex>m~](i9 'LA q `/  7e$(yH#   5V+' `/      &V>~s,@  !)29=???@CFGFCBFUsQ_+)qq]@2.04775/' 6c~L$  .dBrc$x7=1>$C   0]h4 *mE8(v>O& B: B        "&())))(&#""%)/488742148?FMSX[\ZXVVXYSG6,7hKw`z@)*5@FE?71/0253.$#$!   ;v.@/~F     $P#%S%         -KkoN/       7nCd\-G      K Fh"  &IsT( I2gya%=!#"   .n]aa     (W.- O       Aj\;"    7r'8w:"2a#DC T+)PD}w:O-3clib'  8Zn=A>H$-^1z/nq8O <m[,           &OR*       "8PdnmaM5      0Pw^/ %]7\]<{F# #'(%   "       (Nx`<            4[h@   "7JW\XL<,   ,Gef? 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"2"GA@%2+=(0$='Rgs6cX&    aB 4+  '   %    !. T (*!&$$#'#3"+86(3%"[4-9 ,$$WJ #15/%&&&&$$$##u86e&",$/B96!  'Y&]Q$$1\$$$$^ 879 t87UTY,1p8, p0NMTC}l,8a32fe'?},;I |*h!"U{=U-dAY;qr^THk,lA,q IJ,[,ZNv*Fc%0tKB,2!e_-1GH@T?{p!+p#4PU^]9!PE Fe5$2<I hYpz!chxpd;c,pL%u.Vd ,p8pM88p bKepRc2f&laV*B44g\8 _+$.l4>3;>N- NA%PkP<H\fK%g A< KY`}d/)<?$M&+!)#-GU+#?94(/Xz`q g{7"A'"*1&*+*!>'.2*/%$8'C'(V6$)(D 5!0*,.0%+ &$+!`%Cvc=,31'_(<#1?)89//'Ja,-=4N<)__:EV;?'I '%:.*!)32?X>W;@')9+) *6 3O#)*,,Pk<@89.3C7EOCD-$(-10;>*0B%$"%2#&;L\65L# %&#%E3*)-:%E+'H.H5@:,Z"6-IA59#0q+#*/7G4-C('2#6'#7M1%60.4-)&4M#7; K~Km7X,F%!V5g\f@//RPRJ5~@rtbSfiGRaTqAS]WQFnQApj& 3GAGCGCGCGGGGACCAAACAAGTTACCCTACGAATCTGTGGTTAAATAGGCCACTGAAGGCCCACGTGCAAGATAAACCTTCCATGAAGGAGATATATATCGTCCTAAGATGACACACCTTCCCCAGGAAAGGGACTGGGTGACGAGACGAGATGGCATCGATGCGATGAAGGGAGCACTGCCTCCTCTGCCGTCCTCCCCCGAAAAAAGTCTGTCACCACCTTCGCTGCGGGGAATAGGATATGGTTAATTGTTATATGATTTATAGGAGGAAGGGAGTGCCCATAGCCCTTACTACTTTAAGGTAGCGGGTAATAAGGGGGTGAGGCCAAGCGATTAGGAGAGATACTTTCCATAGAGCGGGAGTATGATTAGATGAGAAGACAACTTATCTGATGAACTGGAGATAAGAAGGAATCAGGTGTGGTAGGTTTCTCTTTTCGTGGGATGCCCGTATATGAACCGCCTTTTTTTTTTGGGAGGGTTTACAGGGAACATTTGGACTTTGCTTCATTGCGTAGGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:36:31 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtXASPtXB1PtWB1PtWBCTSJBufT CMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA bDATA @DATA &MDATA &M;DATA &MDATA &MDCHTDSamDySN ɵDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFWO_GATCGTyp!!ɔHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~I#LIMS!!LNTD2LsrPaMCHNMODF  MODL3730NAVGNLNE`NOIS" OfSc)P1AM 6P1RL 2P1WD :P2AM BP2BA  GP2RL >PBAS  $KPBAS  "?PCON  0PCON  .PDMFPDMF"#PLOC *oPLOC &WPSZE`PTYPPXLBRGNmRMXV1RMdN ۚRMdV1RMdXۣRPrN~RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate ɈRunN""S/N%"SCAN3SMED SMLtSMPL%%SPACAqlSPAC"SPACAqlSVER4.0SVER JSVER,,Satd)^Scal@Scan3TUBEF10Tmpr<UserphARphCH":phDYbigphQLcphTRphTR$This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtXASPtXB1PtWB1PtWBCTSJBufT CMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA bDATA @DATA &MDATA &M;DATA &MDATA &MDCHTDSamDySN ɵDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabYFVocXFWO_GATCFeat//XGTyp!!ɔHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~I#LIMS!!LNTD2LsrPaMCHNMODF  MODL3730NAVGNLNE`NOIS" OfSc)P1AM 6P1RL 2P1WD :P2AM BP2BA  GP2RL >PBAS  $KPBAS  "?PCON  0PCON  .PDMFPDMF"#PLOC *oPLOC &WPSZE`PTYPPXLBRGNmRMXV1RMdN ۚRMdV1RMdXۣRPrN~RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate ɈRunN""S/N%"SCAN3SMED SMLtSMPL%%SPACAqlSPAC"SPACAqlSVER4.0SVER JSVER,,Satd)^Scal@Scan3TUBEF10Tmpr<UserphARphCH":phDYbigphQLcphTRphTR-"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqiND$sCN&]n+}G]T[p^<8*JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#ky6Ka;v8e7s]jTj.uFn4o[|.]/;7hHCp}NUg2rM3~1,4Mi921kMYYH|O[nY?KPQh^k 1{sI(J8dM!tqw8&rd+'n,J6v$NU,l9cGY@ZWeo)GjL~7L3"6%4y^.N82`\wmj7-U.*P-*KWeq }X'K2MWhc'kT'_C:rF(5,(?z7b[z`>F3!sY#+~/]m#NA^6h9e *E&]E64Us\zcw&M6TVS)TKQoB+BhwD~ '(CwM@B8{A)PS_W .Nio8m*1fCy]_^avep"iK]0T;7[3VT%7;H{Y+]B7b~;HnkKuaXj`Q Dx6m}>7:I12 d+[Y5xo|[Ez1{zK90l_v]M;~6bI.J:*yEwiOOd&79?Ou4[Y{zsyN}@*ohj4TIx*&`_M%Y_bO?C!.tk,8EE.Om,NeLVWYHjD(y$9eKobs>6I(C.FbZ?`gAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtXASPtXAUDThB1PtWB1PtWBCTSJBufT CMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA bDATA @DATA &MDATA &M;DATA &MDATA &MDCHTDSamDySN ɵDye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT JEVNT VEVNTbEVNTuFTabYFVocXFWO_GATCFeat//XGTyp!!ɔHCFGCEHCFGHCFGHCFG==InScInVtLANEFLAST~~I#LIMS!!LNTD2LsrPaMCHNMODF  MODL3730NAVGNLNE`NOIS" OfSc)P1AM 6P1RL 2P1WD :P2AM BP2BA  GP2RL >PBAS  $KPBAS  "?PCON  0PCON  .PDMFPDMF"#PLOC *oPLOC &WPSZE`PTYPPXLBRGNmRMXV1RMdN ۚRMdV1RMdXۣRPrN~RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate ɈRunN""S/N%"SCAN3SMED SMLtSMPL%%SPACAqlSPAC"SPACAqlSVER4.0SVER JSVER,,Satd)^Scal@Scan3TUBEF10Tmpr<UserphARphCH":phDYbigphQLcphTRphTR