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" 93(.'/*'-:$&8.;)4=46817) //F:4A3/4>)M6$ 9)4&23!4/(3(+5.$(,4* +$"$%'((3*!    #                                                   !"#$%&'()*+,-./0189:;< !"#$%&'()*+,-./0189:;<d,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d82836cd439645fbb8242d62786dcfe5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellPolypodiaceae_K1344_A2_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zORV]hv"6KazEn>i&T~ (E`u|kU;O]$nG }~-8?BA>7/#y_F/ zslfa[UOIEB?>=;8520.-,+)'%#!  !"&,3=HTdv4W{}[8U_4w U ' 1 8 (  #9X6LsB}yvutuvvvx}/\BqvjZF.oL)~zuqlhecba`_][ZXWUTRPNLJHFC@=96310//.-,*)'%$#!%.<Ne&Ryr`SLKMQVYXRE2pbYTSUWZ[[ZVPJB;50./4=K]t!&"}eTH@<:999;<>@AA@>;73-("%3IgA]jeP._B-        &2E^|~\<#!=`z`TXjuT8!     )B`aA&    ,5<=91& #4GWaeaVF4#       9^b>!     *AYmxynY@(#?fcE,  #&))(%# "%(+,-..-------+(%#"#&)+*'""(+,("#.:EOX_cc]QB0     #)+)$       "!  -=HLH>0" 'B]tqZA*!$#    *3995-#    #&&$  5NbnndP9#&1772+##+4;=:2' $5EOPH9&%BazzaD($5CJH>/              *;GKF:*   !$" 'B_v~mT8       .<HNME7(3GW^[N:#(B[mslY?&3Ql}iP8$ !&&"        <^|qU;( "" /I_oskZD/ %9M]dbVC.  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'+*% !!          #&)-27<@CFGIJLNQSUVXYZ[[ZWRMF@;754553/(&<ThttiT;#%;P]`WG2    +6;90!,CU_^R@+     2BLNF8&  %7FPQI;*    !3CMOH:*&;NZ\TE2!  %6DJH>/ #%#  "4GW``VF2 !2AJLF:+      (;M\b_SA,             2BMPK?/  "3EU]^VH9,# #%%"    +9CGD<2'   #6HSVN?,       &A]s}yhN1  $,/+#              n@AHAO$tAѷKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTGCGTTCGCAATCTCTTCCTTGGCTCGATCCGCCCTATAATAATTTGTTGGAATGACCTTTAAGACATTTACAAATGTCTTAAAGGTCATTTTTAGTTTTTTCTTAGACTAATTTAAAAGATGTATTCTTCTATTAAAGATTAGCCATTAACAACAGGAGCTTCAACAGCTGCTAAATCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCAGAAAATACGTTAATGCTAACAAAAGTGCGTTCGCAATCTCTTCCTTGGCTCGATCCGCCCTATAATAATTTGTTGGAATGACCTTTAAGACATTTACAAATGTCTTAAAGGTCATTTTTAGTTTTTTCTTAGACTAATTTAAAAGATGTATTCTTCTATTAAAGATTAGCCATTAACAACAGGAGCTTCAACAGCTGCTAAATCTAGAGGGAAATTGTGAGCATTACGTTCATGCATAACCAGAAAATACGTTAATGCTAACAA1I_v (,=JXmu&-;COW_ku~ !*5BP\gq| %1>IT^jx(3>JW`my #/:EQ]jw $0;GT_ju%0<HVam{    ) 3 ? L W d p {    ) 4 ? 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K V b n x    % 1 G R ] i t      )   #"#2)+& %   %,'"  ''&$+-211'  "+.))+ "( "))'  2.."++."".$""+11''++1+1"1'")"'""+'            )   #"#2)+& %   %,'"  ''&$+-211'  "+.))+ "( "))'  2.."++."".$""+11''++1+1"1'")"'""+'       #U6B$^!6[ b6ZF1PS!a[@0] ^4.1N%+!E-+ aOL(I;>3O&#@MAOGSd!*J:UO2O1\H7ME A5:D -#EV L@ 8 Xc1Z*>B 2QK9F,X:PG:K+C# UKU3XM'.\9b,1CK:aZ?$.@@>*DI8O;=89HIIQ$T]5+0. *Eb(;AL>D#,)]=}Q&N^9`/'j)>RoK{3s[ 6:.;DCnQK1;r@/81B1wrP>d)=3:5"VmX>F .$>:xmfSLddjW Rj94{FC'1u{K] _UO8yn s{w9*4B.V#bh8j >[*X[(o hXe!pF\@_tfRM7@V.1,D}dE;/? h.B1t(=6;*p=; .# =     %  e   /,A '+Ay3(2 1pU pp'0phfCB~8={'<@4mBu^|eMlw 31aywC!JnK5]y8'me$}V6#*'Y*XNNdi|k RS=e D WB0*pZ)g%o*LdGI \$Frs #ph#;%;tu$Gfww:1G;Ea097qE!g-Bm t}-oYubd76AOA&l_{%Fk u0>g+!D:U,-0?$GGAGCGCCCGTGGCCAAAAATACGTCGAACTAAGCGAAATATTATGAAATAAATTTATCAAAACGTCCATAAAGACCTCTGTCCCCCCTGACGGAAGGTCAACACTAGGTTTCCTCCAGGTTACAACCCCGAGTGGAATGGTCAATTGTTACTTATTTTAACAAAGGTTGGCGGTTCCCCCTAGATTCTTGGAGTGCGTTGAGCTCCCACGAGGAGTGATATGCTCCTAACCCCTTAGCGGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:35:44 -04:00AEPt3AEPt3APFN APXV2APrN ɬAPrV1APrX}}ɵARTNASPFASPt[ASPt[B1PtZB1PtZBCTSFBufT yCMNT uCTIDPCTNMVCTOwiCTTL lCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA >DATA DATA DATA DATA #G>DATA #G>4DATA #G>|DATA #G>NDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FWO_GATCGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ɂLNTD2LsrPaMCHNMODF ɣMODL3730NAVGNLNE`NOIS OfScpP1AM;P1RLWP1WDP2AMP2BAP2RLPBAS PBAS PCONePCONsPDMFtPDMF PLOCPLOC PSZE`PTYPqPXLBRGNmHRMXV1RMdN 2RMdV1RMdX;RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate  RunN""&S/N% SCAN3SMED SMLtSMPLWSPACMSPAC SPACMSVER4.0SVER ;SVER,,ȶSatdp^Scal@Scan3TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTR,ՁThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ɬAPrV1APrX}}ɵARTNASPFASPt[ASPt[B1PtZB1PtZBCTSFBufT yCMNT uCTIDPCTNMVCTOwiCTTL lCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA >DATA DATA DATA DATA #G>DATA #G>4DATA #G>|DATA #G>NDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FTab,FVoc,FWO_GATCFeat..,aGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ɂLNTD2LsrPaMCHNMODF ɣMODL3730NAVGNLNE`NOIS OfScpP1AM;P1RLWP1WDP2AMP2BAP2RLPBAS PBAS PCONePCONsPDMFtPDMF PLOCPLOC PSZE`PTYPqPXLBRGNmHRMXV1RMdN 2RMdV1RMdX;RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate  RunN""&S/N% SCAN3SMED SMLtSMPLWSPACMSPAC SPACMSVER4.0SVER ;SVER,,ȶSatdp^Scal@Scan3TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTRo#9:8XY8UXabCjC:AVVu p!9aQzX5f33n fh-OCy~0RgB*c4;%=pZ{d*ya|f|=Ry%`;5m;*/[i&`p'6-_X.){!9Y2]C0nD2,39N]n!afT7+BOJFU'.Qmj*FW1iSx#_1FbI.J:ps\wi;[kt\%ED6vt'5:?S`!;R6{~\dAzmiOO?-|1a^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9KNJ04MYbC*THOpa,VS&sF{LbzMiW~!Tq*Yh-8Z0s&^_XUQLzIQ0%}jx7Z]eth'=o_(@=8Ab;*5"sIVzYoJQ]/({T?HK_eXjK`4]iDs=4z`i1@t3)LDK2N#ti(FsCT[m^.|bEdG[~{?26?K;@WFV>Axe<>/D?*Uq$[-g]A8c[28NL-hdVE~ir4M~uI nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#+"CKjN$87ujVN#4oNYe,<{.gP,{oThnbOu2gHhpqc]lDFIm/TIHVsRt&'sEMm3`lEA_:k9vAMV^@=?]p&q6X+9mB\yE*<^k'eaK|`P@R(RlSR[7}?e=ES77~U?63_iP-2ZE<}|Uo%Z=r*r`sEY?bM:bFs5Iis|K/l3672tXMV^A$+vTE#!#+Xn$yYE!p;|WnT(%Fy1pLze~!Tq*nEg8Z~KI'_2B.*W"i-I7} W5WDt;x.qK|>VRJxG57'9\47.<<_JWT~Q;maHjlUOE6OLhcDW4KD@|v^&Gd\{diO}L6>U{mwYBT3?^NHEDX+9m#k@l'Z9d[TCs]jL'#k=sDa)x+7E2]^DATA DATA DATA DATA #G>DATA #G>4DATA #G>|DATA #G>NDCHTDSamDySN MDye#DyeNXDyeN]DyeNbDyeNgDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT FTab,FVoc,FWO_GATCFeat..,aGTyp!!,HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ɂLNTD2LsrPaMCHNMODF ɣMODL3730NAVGNLNE`NOIS OfScpP1AM;P1RLWP1WDP2AMP2BAP2RLPBAS PBAS PCONePCONsPDMFtPDMF PLOCPLOC PSZE`PTYPqPXLBRGNmHRMXV1RMdN 2RMdV1RMdX;RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate  RunN""&S/N% SCAN3SMED SMLtSMPLWSPACMSPAC SPACMSVER4.0SVER ;SVER,,ȶSatdp^Scal@Scan3TUBEA2Tmpr<UserphARphCH phDYbigphQLcphTRphTR