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" 2* 8%-PJ7=\JgW:$$'")*0! &)7>- #/`~qB6&"   ;="7/&  %- =Mjpt 'X2_{^FMXRsfkh\0I98  ' 0&&*        #' *)+(A'"-%71 *%$7'"%-'"$'#                 d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR3}|ywuPPPIIIfff}}}! !)))211:9:BBBJIIQQQXXX``_gffmmmssszz|~<<<<<<<<;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 608795e946e443f98c1b0fa5e7e24804Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-18_07-51_18793130Seq18251138297_K1346_trnh5fjmadera96-Well%Pleopeltis_villagranii_K1346_H4_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zwz /?Qf+Pv"T,gV?]xnP.w;y8}C yO+ *?Uj}x[>cH.~vme\UOLIIIIIHGFEDCCA?=;865321000134689;>BGMT[clx8^GutIa= /O} gY  *   )> iT?,zuqnmos|5hU5[t~uZ-g. >x#'Ga13DOV\bjqwyywuroke^ULC<51-*('%$$$%'*.3:@HOV\`ba^YSMF>71--1=Pj@kxjeiuyV1 qe[TLE=7447?HS]gov| 6CD:% lWG;3,&"#7T{Uh5fSF<4-'"      )16874.' !$$"  #2CQ\bb\QE8-%   "%')+---+'# ,JpcB) %3@JOPJ@3%  ',/-( -C[p~saL8&   &))&  $3AKOLB5&  &*+'  -Faxv^E. $#4PnnO2 &Gp~V5  ",6@GKIC9-#*;SpvZGEUw dB*"*2:@CA:1&  "'*)% -:DIG@4'  .G`t~s`H0  !!  &+-+% &)("   '*)$  ,<LW\YOA0       .F\jnfT>( -CYiojZC+ #$# !.?O[`ZL9% )02/(     4_Y/   '07:7/#          !#"     +7==6* "!%+-,&  $6FQUPD5&  #'('%%&),-*$    !!$(*(#   &,/0-'      )//+"#,34/& *:EG@2!    &5@GGA6)!*142+!  #()% .8;7, *?Q\\Q@,  (030( &8HPPG7$ %;P`fbTA,  !"!  %+15651+$ !'**'#! !"$#  %.6970&   ->JOK?/  ,9BGF>1#    %)(#*5;91$     1BNRM@/ '07;;6.# #''$  '8GOPJ>1$    #&&$!           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"$$                                       F%@֡AAVB[AKB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTGTCGTTGTAAACACTGTCTTATTGGCTCGTCCGCCCCATAATAAATAATTCATTAAAAATGACCTTTAAGACATTTAGAGATGTCTTAAAGGTCATTTCTAGTTTTTTCTTATTAGACCGAGTTAAAAGATATATTGTTTTTTTATATCATATATTATCCATTACCAACACTACATTCACTTCCTGATAAATCTGGAAGGAAATTACGAGAAGTTCATTCATGCATAACGAAACAGGTTTGGTATTTGATAGATACTACAACTGGTCTGTAGATTGATTATCCATTCACAGAAGGAGCTTCCACGGAGGCTAAGTCTAGGGGGAAGTTGTGAGCATTACGTTCATGCATAACCAAGAAGTGTCGTTGTAAACACTGTCTTATTGGCTCGTCCGCCCCATAATAAATAATTCATTAAAAATGACCTTTAAGACATTTAGAGATGTCTTAAAGGTCATTTCTAGTTTTTTCTTATTAGACCGAGTTAAAAGATATATTGTTTTTTTATATCATATATTATCCATTACCAACACTACATTCACTTCCTGATAAATCTGGAAGGAAATTACGAGAAGTTCATTCATGCATAACGAAACAGGTTTGGTATTTGATAGATACTACAACTGGTCTGTAGATTGATTATCCATTCACAGAAGGAGCTTCCACGGAGGCTAAGTCTAGGGGGAAGTTGTGAGCATTACGTTCATGCATAACCAA5Nds +9CIS^r{ !-5=GT]gs| +6BNXdq "/9DP[hu$1>JUbmy ,6AMWdpz ".8EQ\hs%2>IZfq~   % 1 = H T ` l z   - 8 D Q \ h s    $ 0 > K V a m x    + 6 A L X e p {   % 1 = J U a l w +6BO[gr}'2>KWdp} #.;HT`kw5Nds +9CIS^r{ !-5=GT]gs| +6BNXdq "/9DP[hu$1>JUbmy ,6AMWdpz ".8EQ\hs%2>IZfq~   % 1 = H T ` l z   - 8 D Q \ h s    $ 0 > K V a m x    + 6 A L X e p {   % 1 = J U a l w +6BO[gr}'2>KWdp} #.;HT`kw  .. &+8 ' * """-'");"" 1 '" "")$""++")"".""''1+&"""'"8+                         & ;;++13.++"3133+;3.;33;;;;.;;>;;.;;8;8183;;>>>>>3;;.>>382332222-3;;;,>;;32"3-3;3;;3;32'-2>;;8288;;3;+82&%3#   .. &+8 ' * """-'");"" 1 '" "")$""++")"".""''1+&"""'"8+                         & ;;++13.++"3133+;3.;33;;;;.;;>;;.;;8;8183;;>>>>>3;;.>>382332222-3;;;,>;;32"3-3;3;;3;32'-2>;;8288;;3;+82&%3# /L3.:'$D?=BA\\]a:MXb$ SH 6GE- -'M< $>R!YK?F,:YG.$O$6[\cU/;DUc.<!!H8 F<'`PH /]T RVN&8@+@,L&F3 +9!:SCDc`+ %R25.`7\Q8%\IG$''P%AFS/Q"1TcSR$Q7XF= J()X BX,[#9&?8/R(PJL_O9;*EWNC"/$W,Z(.=#'/FQI;>#Y;Y +5@3ELUUJ:54)14!!+6;;!-VWI`D,L[A"_!\0;MGTC<=!;1DT]SB?,0CL< 8T5&Qq\?!?E Yimd8n!%) NZ1@+Yb4H?5h|y,y D8X @ m"Um<MeD%Uex&zQo; }} C02ZX'@V e7n*a: ewH(O*F;"wj {$l]+qi! |QVs#(;0ofmmtEJ^S^&}/},;[Acz?IV ,1jVt=MSZ&`(;Ix_ cK `JlYQ?Hp$XDqf|R^eXK@{i?1t;:0Ldd:8) 3*-+)7$v4ACRA6=,)2 P!5S.) K+9= 9HI*   0 " ,D T+AjfB7 > 6'e3    Z#,p8?8d7;6lxpKxB)5#}K,6>,Y|Q7wttr aynv$pSv^'0lamLFIjVR<2T.Xzdsr_zW#<2K8QmfVSak,yp&Pxh;hl)X zW46OtI \B,pO(,DuEf ZH1]FUZNR  Z)`V<!iBYm^txKp*`ClrP?;G\qxFXdmn[ \C2&V[g; F%\WZo5!=Mbj:)b:";WkQo$`Y|W\Fx^xs?}w3\i--R&8bAO9>0GVq7p@{mn-/B_n[qcHT'LD2dY]OxDBkE`J'+AGGAGCGGCCGAGGGGACAAAAGAACCGTCGAACCTGCGAAACATCATTTATGAAAGGGTCTTCAACATGACCGATATAGCGTTATCCCTAATGCCTGGCAGGTCTTACCAAGTGGGGACTTAATCAACCGCGCTAGCGCATAAAAAGATATATGATATCCCGTATAACAAGGAGGGTGCAGAGACGGTAACACTGGAGAATGATTGTGATTATTCTTAAAGCGACAAACACGGATGCGCAAAAAACGGAGGATAGAGATTCCTCGTAAAGTAAGGTGGGTGGAGGAACACACACCGTTTTTGGGGGCGCAGTAGCCCAATCGAACCAGGCAATGCGTTGGGTATCCACCATGCGGGACGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-18 09:21:30 -04:00AEPt3AEPt3APFN 3APXV2APrN APrV1APrX}}ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS=@BufT"DݲCMNT íCTID݈CTNMݎCTOwݢCTTL äCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"D"DATA"DfDATA"DDATA 'QN<DATA 'QN4DATA 'QNDATA 'QN"DCHTDSamDySN ÅDye#DyeNÐDyeNÕDyeNÚDyeNßDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFWO_GATCGTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~;LIMS!!úLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS OfSc0P1AMh/P1RLh,?P1WDh1P2AMh7P2BAhh:OP2RLh4PBAShh"gPBAShh PCONhh*PCONhh)oPDMFPDMF PLOCh&PLOCh#PSZE`PTYPݪPXLBRGNm݀RMXV1RMdN jRMdV1RMdXsRPrNNRPrV1RUND RUND RUND RUND RUNT 3 RUNT   RUNT RUNT ,Rate XRunN""^S/N% SCAN3SMED SMLtSMPL&&SPACMSPAC SPACMSVER4.0SVER =5SVER,,Satd0^Scal@Scan3TUBEH4Tmpr<UserphARphCH phDYbigphQLcphTRphTR1eThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN 3APXV2APrN APrV1APrX}}ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS=@BufT"DݲCMNT íCTID݈CTNMݎCTOwݢCTTL äCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"D"DATA"DfDATA"DDATA 'QN<DATA 'QN4DATA 'QNDATA 'QN"DCHTDSamDySN ÅDye#DyeNÐDyeNÕDyeNÚDyeNßDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFTabKFVocKFWO_GATCFeat//K[GTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~;LIMS!!úLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS OfSc0P1AMh/P1RLh,?P1WDh1P2AMh7P2BAhh:OP2RLh4PBAShh"gPBAShh PCONhh*PCONhh)oPDMFPDMF PLOCh&PLOCh#PSZE`PTYPݪPXLBRGNm݀RMXV1RMdN jRMdV1RMdXsRPrNNRPrV1RUND RUND RUND RUND RUNT 3 RUNT   RUNT RUNT ,Rate XRunN""^S/N% SCAN3SMED SMLtSMPL&&SPACMSPAC SPACMSVER4.0SVER =5SVER,,Satd0^Scal@Scan3TUBEH4Tmpr<UserphARphCH phDYbigphQLcphTRphTRAvotqUaKOfx&i1PBEe>2}?6i<#~p$F0Ns>yEOikaw>6?|$lQ%:XGT8P/kdSPcgoC#Q9p_]c3-doFOACEDFi34TKs;6R&w^LgdOZR<0+M??^V$Y#L,jpJ6~=D:WqPKw0j9eKobs>6IxO\JpSbwEiv58u@B\$nq%StumfXr9lKnD8Y~Csk%pM_T![sGj`X0?888g2lI>iV=1SbN+b)c7W`XD8NT !T;b^7ghRS|6dc+GHO5QqEi>o.B3@dSOK[-z|G0_fs_c&a~4~kJF96YZ{sb: h4qKl}T2#:Da^?vftu3ky+MGPpF~qoMY'H\W+|v^.PZPBE YXp!R*lVinJilSZrB]'G,lwdRadJsV&=o?eU&GUkJZm{n(cbH?6+]!:L"ZU+$}.k>Qu3NM|5O-U[ds=:I12v-T)D@0vrs|%3lC(`J8daxF![pE)}Of'PJA}*4d#aY&1FDHYWm;2FVcxS3"6%4y^'a,k){hI{?7.N{C]QHW>ycPX*F8Ba+b8E~$3.-Zl7'p=R .*}Lq{F}u^zq+xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_MghW~^(';_/C]2ZE<}g6M>P:Y'((nIcqM2kK1udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuUIcTA?i]8S]*|QK:}$OEH3<("Hf.Q+s3TtGK><3$G,lwd-l#frL?*Y^gM*yAEPt3AEPt3APFN 3APXV2APrN APrV1APrX}}ARTNASPFASPt4ASPt4AUDT[nB1Pt3B1Pt3BCTS=@BufT"DݲCMNT íCTID݈CTNMݎCTOwݢCTTL äCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"DDATA"D"DATA"DfDATA"DDATA 'QN<DATA 'QN4DATA 'QNDATA 'QN"DCHTDSamDySN ÅDye#DyeNÐDyeNÕDyeNÚDyeNßDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT &EVNT2EVNTEFTabKFVocKFWO_GATCFeat//K[GTyp!!dHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~;LIMS!!úLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS OfSc0P1AMh/P1RLh,?P1WDh1P2AMh7P2BAhh:OP2RLh4PBAShh"gPBAShh PCONhh*PCONhh)oPDMFPDMF PLOCh&PLOCh#PSZE`PTYPݪPXLBRGNm݀RMXV1RMdN jRMdV1RMdXsRPrNNRPrV1RUND RUND RUND RUND RUNT 3 RUNT   RUNT RUNT ,Rate XRunN""^S/N% SCAN3SMED SMLtSMPL&&SPACMSPAC SPACMSVER4.0SVER =5SVER,,Satd0^Scal@Scan3TUBEH4Tmpr<UserphARphCH phDYbigphQLcphTRphTR