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!!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8221643bf7854841ba5a2d15f0dbc5c1Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellPleopeltis_K1344_D1_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z /CXl(Lp 9eEyKs9N\a\L5}S'X^'s]ND@@@AABFO\l}yo_I+ 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Be@AAQA~KB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTGGCGTTGCGACATCTGCACATTGGCTACGAACCGCCTTTATAATAATTTGCTAGAAAATGACTTTTAGACATTTTTTCATGTTTTTAAGGGTCTTTTTTGGTTTTTCTTAAACTGACCGAAAAGATATATTGTTTGTCTTAAATATTAAATATTAACCGCAGGAGCTTCGACAGCTGCCAACTGTAGAAGGAAATGGGAAGTATTGAGTTTTTGCATAACGCATAACATCCTTTTTTTAGTTCCAAAGCTCCTATACAATCACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCGAGCGGAAGTGGCGTTGCGACATCTGCACATTGGCTACGAACCGCCTTTATAATAATTTGCTAGAAAATGACTTTTAGACATTTTTTCATGTTTTTAAGGGTCTTTTTTGGTTTTTCTTAAACTGACCGAAAAGATATATTGTTTGTCTTAAATATTAAATATTAACCGCAGGAGCTTCGACAGCTGCCAACTGTAGAAGGAAATGGGAAGTATTGAGTTTTTGCATAACGCATAACATCCTTTTTTTAGTTCCAAAGCTCCTATACAATCACAATCAGACCAAAGTCTTATCCATTTGTAGATGGAGCTTCAATAGCAGCTAAGTCTAGAGGGAAATTATGAGCATTACGTTCATGCATAACCGAGCG6R`e+5HW_e~ +>M[er~(3HRZgu} !.:EO[ht~ $2=JTdo|  ,;FR^iv+7BMZfq}$0>LWdq}  % 2 > J U c o {   $ / ; H U ` l y  ! , ; D O b m z    * 6 C M Y d q }  " , : G R ^ i v *6AM[dr)3@KWep| $1>JVbmz #.:L[p{6R`e+5HW_e~ +>M[er~(3HRZgu} !.:EO[ht~ $2=JTdo|  ,;FR^iv+7BMZfq}$0>LWdq}  % 2 > J U c o {   $ / ; H U ` l y  ! , ; D O b m z    * 6 C M Y d q }  " , : G R ^ i v *6AM[dr)3@KWep| $1>JVbmz #.:L[p{   '                    " '    ""                   "    &(&8';.8+;  .1.$((++1+38.&.88;"2#"2'13+  12#("$$"&+++,02'"823113+!"     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Z0 &0[$<VROC0(9UTUc/+H2W&aJ?K[,/6GA T<U%6:=+Nb(%\+5PF%b.%>-,&*80?L.+QRL<6SXN>W7(%I8a"Z4QDJ=cZX\#2 M"'F ',X78-:&K(U14W$4 -<H2W E(#"F4 , (aE`BC<7),3^*c/'T\P)S NX<C_9N]$(0S;I*LDL-b=J 85H><c!(3J]/@Q,W8WN[Y!+<   Qwq5h-|E9g.f4{}3t>&[mkWs:z Q`YChE(2<4Y<>[!dXU@H*#wd$e]|V[gx&(V[6+M*O-fwYy=) R!|UM^A+Mc~:DXqh#brH K 'd4[2y73mV*B`6M|#j2}W( .FM `&Fr jHTnaCb\EWId=IB, 8c/K[D6 L-B*iq-I] G)eKv S#!(!G @{S   n8  ' ) k|wxr]1O$N!"% )%&V<$^0pdpSppUzc p`,MRw[:{WvtK#1#bpObtP<TS  6Ix6z7.>A<K\)p,bsycSE+21$Q8J-eMlEnUAeY6CSV|fdS*B]LI;y]= <D\5?`AVa4pU48_p{g#fw3wW>I/K- e}1Q)h>\y+U!x7$(zOuEvc;rr '}so0&$|w^u9 4"T Y e Ya4n=[j)iEy5#cK>x2VR y7Xnci% S001 7X0^TCu#jhp5nWUP3tZbMxNxQG t{Z#IyUui*k%_uqP]kks?HcuAGGAGCCGCCGTGAGACCAAAATTCCGTTGATAATTAACGACGATATTATGAAATTATATCCGCCTTCAAGGCTTTACCAAAACCCCCCAAGGAATCAACCCGAATGAACCTCCTGCTACTTTTCCCCCCAGGGGATAGCAAGGATTTGGGACGCCTACCGTAATTAATAACAGAGGCTTCAATCGAACCTAGGTCTGGATATGCACTAGACCCACAACGTGCCGAAGATGGCAATCAAACGATCGGGACTTAAAAAGGATGCCGGTTTGGGGATGACTTACTCAGCGAGACAAGTATAACTATACTCTATAAGTGGCGTAGGTCGATCTTGGCCGATTATAGGACACGCAAGCGTGAACCTATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:35:38 -04:00AEPt3AEPt3APFN PAPXV2APrN tAPrV1APrX}}}ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTS=BufT ACMNT =CTIDCTNMCTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA &lLYDATA &lL:1DATA &lL DATA &lLDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȪEVNT ȶEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~;LIMS!!JLNTD2LsrPaMCHNMODF kMODL3730NAVGNLNE`NOIS OfScTP1AMl/,P1RLl,TP1WDl2P2AMl7P2BAll:P2RLl4PBASll"`PBASll PCONll*PCONll)|PDMF9PDMF PLOCl&PLOCl#PSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N% SCAN3SMED SMLtSMPLSPACAnҡSPAC SPACAnҡSVER4.0SVER =vSVER,,~SatdT^Scal@Scan3TUBED1Tmpr<UserphARphCH phDYbigphQLcphTRphTRfThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN PAPXV2APrN tAPrV1APrX}}}ARTNASPFASPt~ASPt~B1Pt}B1Pt}BCTS=BufT ACMNT =CTIDCTNMCTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA &lLYDATA &lL:1DATA &lL DATA &lLDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȪEVNT ȶEVNTEVNTFTabKFVocKFWO_GATCFeat00KGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~;LIMS!!JLNTD2LsrPaMCHNMODF kMODL3730NAVGNLNE`NOIS OfScTP1AMl/,P1RLl,TP1WDl2P2AMl7P2BAll:P2RLl4PBASll"`PBASll PCONll*PCONll)|PDMF9PDMF PLOCl&PLOCl#PSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N% SCAN3SMED SMLtSMPLSPACAnҡSPAC SPACAnҡSVER4.0SVER =vSVER,,~SatdT^Scal@Scan3TUBED1Tmpr<UserphARphCH phDYbigphQLcphTRphTR6}k1$AZ}E]bZ&[VGgPH/#8|.IQK%=G{}(-TPrbJ32Qy]xwY>2hpu:In8nZZ*W]U_I[Ddg/.9u2jDTWy2>y^q3Th$Y thENWNv3[My I3)yfuq.$>2tp(kA;M4[{nYD3RhW~^N@.]nUHw>VihGJNM pzC2:MMo/Q0-A(2HTrhV KVSFsA b5}8 v/,J6v`ZeW\j//u@8Q CKOjLH#[,bTmq/*7ag%gcmeLMaCG !9W&a:OcPC]LVn!<|'{XW,@|sF4GG\}d M;Y@iGtX[3>>B%P^gWyR;uIkrE-O)\rS|5qk`T1jdRC WIFM/|R^8_gJjo[h<+ZH~\;WRnc!|`k\%{ Tv*PK@|Q$OEHbX\"B-8'i2{&[y3( ]'ngg61C8dk7YZFU?-4$&LkfG;7oF`|zvn[Y<,h`+m&wE6Gz zF/"Wtixy$|FRrtlqaxkabCDk$OE-`{siO}L6iyZnsz3P<}u^ZqNX+9_e V&&OE6OLo X:QJf9/qwAxe<][#m*ZY8|k_R.AQ>UR6? sdWUx>|h)|B]AMkG~H^S]8Jb%`MU3\U5|8e7s+]i@C,TAq;vn;l|t"eAK^,0t;L,p2i#G&RcP3eZHBa+b8ENIRJ*Wi4GG\}d M5_@{&]t[X&rd+'n#wCdNmbcnPkIp?Hdsgbk1^DR!_Tu#,xHL?iCB0'~nRnX@EV$2kE}:>&ZgL7:tl|y8UWb!4s'B{1@!&=,ZAbwlTi&2])J3)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9vAMV^@5oOC&qN1;g8kY+-^m]ZOQ>\G`V7%jxAEPt3AEPt3APFN PAPXV2APrN tAPrV1APrX}}}ARTNASPFASPt~ASPt~AUDT[B1Pt}B1Pt}BCTS=BufT ACMNT =CTIDCTNMCTOw1CTTL 4CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA &lLYDATA &lL:1DATA &lL DATA &lLDCHTDSamDySN Dye#DyeN DyeN%DyeN*DyeN/DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȪEVNT ȶEVNTEVNTFTabKFVocKFWO_GATCFeat00KGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE LAST~~;LIMS!!JLNTD2LsrPaMCHNMODF kMODL3730NAVGNLNE`NOIS OfScTP1AMl/,P1RLl,TP1WDl2P2AMl7P2BAll:P2RLl4PBASll"`PBASll PCONll*PCONll)|PDMF9PDMF PLOCl&PLOCl#PSZE`PTYP9PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N% SCAN3SMED SMLtSMPLSPACAnҡSPAC SPACAnҡSVER4.0SVER =vSVER,,~SatdT^Scal@Scan3TUBED1Tmpr<UserphARphCH phDYbigphQLcphTRphTR