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JKLMNOPQRSTUVWXYZ[\]^_`abcdeijklmnopqrsJKLMNOPQRSTUVWXYZ[\]^_`abcdeijklmnopqrsY,,,III^^^{{{&&&999DDDXXXaaavvv      ***333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}zywtrqonPPPHHHeee}||   '''0//877@@?GGGOOOVVV]^]dddkkkqqqxx{}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: fed816b7f69042578cbca95067a95f70Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_22-36_18703130Seq18247138284_K1345_trnhjmadera96-Well%Myriopteris_lendigera_K1345_H11_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zfhkqy%8K^r3Qq"IqCj &+,*&xP%`-lM/ u]C*}zwussssttssrqqpomkifc`]ZWTQNKIFDDDDEGHJMPTZ`gq|#Bd "7HTYWM<$b@!8iP?t&~L  V  5 . 1 Hp"-`0 }vplhea^[XVTSRSSTTSPLFA=;?GUfy(Q}/Rp{dI(}V<2:RsF}Q914;@B?;7555667889863.*&#"!!  *:Pl,Olt_I4$(4>DC;,fQ@5.*)()*,036887668>IYp,<@6!mQ;*  %*/2332113;GZt 3TjpgM(qP6#        $(+,+'!  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(;Ri|v_D*        1@KMH=0"   ,6;:3(/?MTSJ;*      &*)% !$&&&%$$$%(/;LaxvsyocZURNJF@:4.)$   "#  (1660&          ,;DE?2"      )253* %1771'  *375.# "3CNRL>+ &;KSRH8'  1AKNI?2)%&*.0,%    *3771(  "*/0,$    %4@GF?3&  "5HWbeaXL?3(  2DQWSH8&      *241) !0=DD=1#  "&((&# 0AMRMA0  ',*$  #,10*    '4@GJJGC@?><82*       &3AMV[ZTH9)   "$"  !*8I[kstk\J8(!%()'"   (9EKI?0   *LrnG%#&$      d1A)A'}Ae_AbKB.bcpKB_3730_POP7_BDTv3.mobtermAAGTGCCGCGAGCGACACCTGTCTTCCTTGGCACGTACCGCCTTTTTCAAACTTGTTCATTTTTAATAAAATGAAAAAAATAATGACCTTAGGTAATTAGGTTCCTGAGGTCATTATTTTCAAGTTGTTATCCTATTTATATAAATTTAAATAATAAACTTATAATTAAAATATTAACCATTTACAGAAGGAGCTTCAACAAAAGCTAAATAAAGAGGGAAGTTGTGAGCATTACGTACATGCATAACGAATTCAAGAGTTAAATCTCAGAAGGATATTCCAACTCTTCTTTCTCCTCAATTCAAGGTGGGAAACTGGTGACCGTGAGATTGTGACAGGCCGTTATTCTCCCCTCTTTTCGTTCTACCGTAGGAACCTTCCATTTTCATTGGACACCAAACTCCATCTTCCGAAGTTGAAGGGTTTGGTATCCAGTTGTGCTGCATAACTAGACGGCAATTATCCGTTTATAGAAGGGGCTTCGACAGAAGCTAAGTCTAGGGGGAAGTTATGAGCATTACGTTCATGCATAACGAGAAAGTGCCGCGAGCGACACCTGTCTTCCTTGGCACGTACCGCCTTTTTCAAACTTGTTCATTTTTAATAAAATGAAAAAAATAATGACCTTAGGTAATTAGGTTCCTGAGGTCATTATTTTCAAGTTGTTATCCTATTTATATAAATTTAAATAATAAACTTATAATTAAAATATTAACCATTTACAGAAGGAGCTTCAACAAAAGCTAAATAAAGAGGGAAGTTGTGAGCATTACGTACATGCATAACGAATTCAAGAGTTAAATCTCAGAAGGATATTCCAACTCTTCTTTCTCCTCAATTCAAGGTGGGAAACTGGTGACCGTGAGATTGTGACAGGCCGTTATTCTCCCCTCTTTTCGTTCTACCGTAGGAACCTTCCATTTTCATTGGACACCAAACTCCATCTTCCGAAGTTGAAGGGTTTGGTATCCAGTTGTGCTGCATAACTAGACGGCAATTATCCGTTTATAGAAGGGGCTTCGACAGAAGCTAAGTCTAGGGGGAAGTTATGAGCATTACGTTCATGCATAACGAGA6Tdn #07AQX_l %1;JVcoz %2?GP\gr},:DQ^ku (7CP]hs(5ALXdq} !+8GS^kx !-8CO\gt   0 9 E S _ k v    ( 5 B P [ i u    ( 2 A M \ g t }   ( 4 A M Y f p |  " - 9 E R ] i t '3@MXdp{ %2=IWcn} $1?JVbny #/;FQ\ju*6CNZeq~ %1=JUboz "-;FR]jv )7CNZis+4@LWer~(4@JWbm{ $/;IT`nx  -8CQ_j6Tdn #07AQX_l %1;JVcoz %2?GP\gr},:DQ^ku (7CP]hs(5ALXdq} !+8GS^kx !-8CO\gt   0 9 E S _ k v    ( 5 B P [ i u    ( 2 A M \ g t }   ( 4 A M Y f p |  " - 9 E R ] i t '3@MXdp{ %2=IWcn} $1?JVbny #/;FQ\ju*6CNZeq~ %1=JUboz "-;FR]jv )7CNZis+4@LWer~(4@JWbm{ $/;IT`nx  -8CQ_j !  *+"/4     &      $"            "     "              (."'".&.+++"8!!;1#23;8;3;;22++1'+21#.221'"&.+"2+++2+2''1.&.&." 018'.1"'".+..(.12!""""++&"+$")%+(1+''"%".21;13;>..'''"""18+&+1"&#.+';'&>31.'' 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SN4F`=#"B9#D=R84 GR ,&1Cb8RC7_.Gd[0\V))! / (!I`]-P] 1PcIZT9N+b8B'6_(!0CC@TA[ 1+Q^ ?^- *R:XN&>EWWZ+7(%//3d-VMU%c4@ IZ1_cOb `A= DH$U M<62 $Z` &,T4S[5U2*;1(DJ@L@p5`9 fSK{z*7Eex7'.z@qq;9H{'0QbZYM6p\~\4gd#$j < -^=5Mu~ 0GLk%^q0:_|1+ ,9s^>(XA|!rO.o[u#(fJ@|f!B Ee;W3bqGm[Xfhw F"oq@-)Q7ksU4e-)"f?/Bx59[c fAY6&wH[%mTDBwcAgvi]]M)c3U yW wWOQ@>s%8_@i*BY!y.h&  HBtLFr@J(-X 6f^Qh,n U))^ `In<0VM5:VO}]trm85[_aPrV~I ,eXRS#3aOOH1=Wio)+"?'92$7U <E1 M!b%H4 ),$ /8F$@< ?e#Z'5QtTR+$-=48V )+3A,Z]9;iv,#o"'; )Q6m+' +F  A(!_k  $)F+!( !0&3! +z$UM4;'DGY/.T;%9<h`';:(([dJ)hww O/ 8;s/JC&@(MTD8> R>},: RO^:/O+Gp3{"jEH/F_kw%y^%8GO<"(ml~qj:b-~]'`'m!&tU\Y>X 3WzPq o^+JL'Tp%ZTQ1Q,*}~ \8rPnH(mVQH0d@;J3 |+"pq.')A6O~yBTs(jp ~T|9/cVsw\4C<YNG{6'r#&52GJHY$w,ykzijp,ppYZ:s{}LY(,pppbXRbpOYOj?^]n" n$~_5\pCth9TS>*VB~z;_Z3'- T_Bu Gq |D#W%z`lQ>~?co|F_f<&f~dJ!aO>I gk9KV_7{q.N_1>vDF9- 8o[$v+8miA'+?v'(b:sGtxD&nSw :EddjWl=@jVa>i9z;bkT`K#r*k(nvZ6cnY>u rCb|b&Qv/5Ld8I^~PEa@Q)"i6>` ] ^V;5QHwz*-xwBw#:@-;@<Vds:_uEkXxMS(IdGGAGCGGCGCGCGAGACTTAAAAAATAAGTTACACATAGCTGAACCCTTCCAGAAGCTTACAAATGATTGCAATGGTCCTATGAATTACCCCTATCAATAACCATGATTTCACAGTGAAAATCCAATCCTAAACTAAATACGTTTACATTGATCATTTAAATATTAATCCGGTAACCAGGAGGTGCTTCACGAAAGGCTACGTTTAGCGGGCTGTTTTAGCCAGTCTTTTGGGGATATACGAGGGCGAAGTACACGAGAAATTGCTCTTTGGAAGGTAAAATTGGTCGAACAGGGGGACGGCTCGAAACCCTTCAATCGTTTACGTGATACAGTTTGTAGTACACACTGGAGACAACCGATCCTCCGGAGGATGGGTCAATGCAAGAGGCTCGATTTGGTTGTTTAAAATTAGGTGAATCAATCAATTATAAACTCATGTTCTTTATTTGGCGGCCGCTAAGCAAAAACTCCCCCCAATGGGTTGGGAGGAAAGCAAAACCCCCAGGTAACATGATTGCGATACTCCCATGCCAAGCGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 00:07:44 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS;BufT'NݐCMNT ÍCTIDhCTNMnCTOw݀CTTL ÄCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'N2DATA'NDATA %{J$DATA %{J1DATA %{J|DATA %{JDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FWO_GATCGTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~:%LIMS!!ÚLNTD2LsrPaMCHNMODF ûMODL3730NAVGNLNE`NOISOfSc'P1AM4';P1RL4#P1WD4+oP2AM43P2BA8 P2RL4/PBASQPBAS7PCON PCONPDMFPDMFPLOC4PLOC4kPSZE`PTYP݈PXLBRGNm`RMXV1RMdN JRMdV1RMdXSRPrN.RPrV1RUND RUND RUND RUND RUNT $RUNT RUNT   RUNT  Rate 8RunN"">S/N%SCAN3SMED SMLtSMPL&&SPACAuSPACSPACAuSVER4.0SVER ;SVER,,Satd'^Scal@Scan3TUBEH11Tmpr<UserphARphCH2phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTS;BufT'NݐCMNT ÍCTIDhCTNMnCTOw݀CTTL ÄCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'NDATA'N2DATA'NDATA %{J$DATA %{J1DATA %{J|DATA %{JDCHTDSamDySN eDye#DyeNpDyeNuDyeNzDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT%FTabJ FVocIFWO_GATCFeat//IGTyp!!DHCFGCEHCFGHCFGHCFG==InScInVtLANEQLAST~~:%LIMS!!ÚLNTD2LsrPaMCHNMODF ûMODL3730NAVGNLNE`NOISOfSc'P1AM4';P1RL4#P1WD4+oP2AM43P2BA8 P2RL4/PBASQPBAS7PCON PCONPDMFPDMFPLOC4PLOC4kPSZE`PTYP݈PXLBRGNm`RMXV1RMdN JRMdV1RMdXSRPrN.RPrV1RUND RUND RUND RUND RUNT $RUNT RUNT   RUNT  Rate 8RunN"">S/N%SCAN3SMED SMLtSMPL&&SPACAuSPACSPACAuSVER4.0SVER ;SVER,,Satd'^Scal@Scan3TUBEH11Tmpr<UserphARphCH2phDYbigphQLcphTRphTR0'"sIR*"$JQ`Q5f|{ce+dr6>svpkx5yu(p4-dId_$_;zay@Q>ko2Uz| Q-M2"ZwclQ[vL}W$620e:jA:>y7LIG!Hgn+1Ab;*5" ~Jz!#fn2+Xb[l"G>H1,Cz#Z|#e1a_y`[qD|3DG\TcpP.!3WN#4LdCh2d'jw~| imyZSH,6cXM{3!dX!%~Afzv*Cy KQI@'.%Nz7R@X`@kjJ}B~xGH$i*8j|]]u.ibu:,n_SUgi8i(=l27&mWAeAT%{AB02oGhnW"KuQtb'J!>yHPx%bg*p/Ygn]]c_0f4[42g|5[S*.J-omMOxkYEZ&dAro XSr$LVYyo6-I1]f)yA@r9#(2g|ea}w*ohj4Mj2Y6YZ_SGmm{k=!.t;Z\3|7hhSc%bVm? : wT_{.BWr4O%\4G(^oL%i-{mwYo{A(PmE$ww*{we* _ZZ^k_o5qS+SP7T]^q;,`h?&X`hB`}_AvG9 HtWo0<8WFoe~8WYH!KPk;+s4U5hf6]X?K90lVSUFGsbrudrV3]wnF){xD\4AFU?-4$&#S%TwLhl+9eZ>mE#5!y|\RVUTtlrml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_|owoqiY.] z,&0@1>X`]yVlX/-f>P5CRV1]cKb%_av;Ue0eTu$@i&L q;I#yiAX+D!2pQ?@H xI>t1,7`nP@>/D?**s$[-o_}4]'Z5P%?p M;8'i2{&[y3( ]'p8NpdUuk} 9`^avedsYK%FyP\[38f6Tx*&`_MSS/N%SCAN3SMED SMLtSMPL&&SPACAuSPACSPACAuSVER4.0SVER ;SVER,,Satd'^Scal@Scan3TUBEH11Tmpr<UserphARphCH2phDYbigphQLcphTRphTR