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"%"% $,( "!0 "$,'%' 1 %>& %$""!)&# $!*$(+   "#0 !) , !"#$%&'()*+,-./ !"#$%&'()*+,-./d,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 72a4032ab1de4312872ec72374e4b229Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellAmauropelta_K1344_B5_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z0H_u)UBx: qq%5 3 ~ " ? 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"5J_q~veQ<)      '8HU\]WK=."!$&%#"$,9Latumlswmilvrhdgoz|m\NGIScr~{n_SOUdw{gVNP\oubTOUbrtdVOSbx~fSKLVblpk_M;+ $$"!-9ADB:0' !&,01.*&$$',01/*"   !"   )6COY`dhls}&9Mbz  w\A(n`XW[_a^WK=0& "'+/.+%!!      '-243/(!#'(&"        #&%        $$            %,39:6/&'07=ACDDFIOW`js|wnkmprqk`SD7.(&&())(&"      ""                                    &,2674/& #" !!     "$%&''%    "*158864211369;>?>=:62/,*)))(&#           !"!                    %+.-(!                                                                     !"               !       ! "#"   "#!#%%#                     "!                               @cA!JASA!VKB.bcpKB_3730_POP7_BDTv3.mobtermTAAGGTGCGCTTTGCTATCAGCTACATGGTACATCGCCCCTCTGTCATTTGTTGGCTCCCCCCCCCCCGCGTGGAGAAAGTTTTCTAAAAGCATAGAATCTTGGGTCCTTGGGTGAACACTTATATTTTTGATTCATGGATTTCAATAGAAAAAAGAAAAACTTTTGAATGAGGAATGGACTCCTTATTTTTTTTATTTAAAATAATGGGGGGGGAGATTTCCAACATTCTTGTTCTGGAAGTGGGAAAATGAAACTTCCTACCCATCCCAAAAGGATATTCCAAATAGTTTTCAGATTCAAAGGTGGGGGTGGGAAACTCGTAATCTTGAGATTGTGTTTTATTTTTCTTACGTTCTACCTCAAGAACCTTAACCTTCACCGAAATGAACACTCCCCCTCCCGATTTGAGAGAAATGGTTTTGGTTTCTGTTTGTTTTCCATGAGGAGGGGAACTTTTTGTGATAGGAGATTGAGCCGAAGGTAAATCTCAATCGAAAGTGAGATCATAACGTTTATCCTCACAATACAAAAAATAAGGTGCGCTTTGCTATCAGCTACATGGTACATCGCCCCTCTGTCATTTGTTGGCTCCCCCCCCCCCGCGTGGAGAAAGTTTTCTAAAAGCATAGAATCTTGGGTCCTTGGGTGAACACTTATATTTTTGATTCATGGATTTCAATAGAAAAAAGAAAAACTTTTGAATGAGGAATGGACTCCTTATTTTTTTTATTTAAAATAATGGGGGGGGAGATTTCCAACATTCTTGTTCTGGAAGTGGGAAAATGAAACTTCCTACCCATCCCAAAAGGATATTCCAAATAGTTTTCAGATTCAAAGGTGGGGGTGGGAAACTCGTAATCTTGAGATTGTGTTTTATTTTTCTTACGTTCTACCTCAAGAACCTTAACCTTCACCGAAATGAACACTCCCCCTCCCGATTTGAGAGAAATGGTTTTGGTTTCTGTTTGTTTTCCATGAGGAGGGGAACTTTTTGTGATAGGAGATTGAGCCGAAGGTAAATCTCAATCGAAAGTGAGATCATAACGTTTATCCTCACAATACAAAAAA38Oex )1@LV`r(3;M\es{*4?IS^jt $0BR]gs&1>IUao{ *6CNYer~(5@LVbly !.:FQ\iu   + 4 A O ] h u    ' 2 > J V a p {  # / : G T ` l x   " . 9 E P ] i v    & 2 > I W c o z ,9CPZiu&3@KXco{'0=IS`kx$.9ES]gu(6BO\eq}$1=JVcny #/:ER^ju)5AMXfq~(3?KXaqz !.;DS_kw!,7DO\ho'2>OYfp~38Oex )1@LV`r(3;M\es{*4?IS^jt $0BR]gs&1>IUao{ *6CNYer~(5@LVbly !.:FQ\iu   + 4 A O ] h u    ' 2 > J V a p {  # / : G T ` l x   " . 9 E P ] i v    & 2 > I W c o z ,9CPZiu&3@KXco{'0=IS`kx$.9ES]gu(6BO\eq}$1=JVcny #/:ER^ju)5AMXfq~(3?KXaqz !.;DS_kw!,7DO\ho'2>OYfp~        %  ++,$.2%"(+&"+          "                               "                                                                       %  ++,$.2%"(+&"+          "                               "                                                                W5& U@FE.[O3-D$_9YH[ZA5!V;IV Q*%C#]&#244S0X^_ )'99\&.3B3IcESK=S7UUc[:H4W1I.S58SG(F <) /3H/ L7ZCOXV9+C3PDBRYC%.^RPB`b<,PH4R![5F@<&DNM@C"@Y7:a KYb=7GQ M6R!cS0 #;C O@`S2>G1>5QAV3KX1 9%K*aH],;K7AAGE9K^@P4C 4 82L_aAF!b&:L]7DFW]GG_S#" H-=>LK('&c 4aPYQYXb/U`F`Q4Nc.%5/7 K\;#Z_PI6QGD4/J41=X93AM(QE F<VLC.ZSQ4--BGJb[4a5*-*;W$1M$]!E# Q1J4[TP/D(*& 3&4Q !c9ML/-c]L`@,P5. aRTQ#8NV)H^E;S8(MYV*>>93,  W:)+oF ,w8 @ Q-aH]b"uWi5y),5LRG,1$L;t+ w@2C!~zo /$VEk0[k<CnUd2G?)/"MCJH(nGl|NTQeI}T 6 t'4Q [moiV4gXLS54N1j;J 5eHfw6[G  af^mMP&~ce`X[R!jX b1WT$zi?xnVQ?PA/1##DGqn n4L J1Md1N\L}~KcbbJVKo   q0$3isr uqey@2 TUWa_d n-F)Y$ X@ `uv `G[@Kx' ;?gCR~gQDJLT^z}EBHD.Kz`PrU^eOgMMljkq7KD2!.()/-& ?:&914 0 R3..uh : ?oL?O!&7Q,> 1 Ez$  a6R.>Nh1-+ / `#PC7 ,?IB'P )& a+y;HEx4861) ATE )jx(!Z:9\4.,bv2/MJ#T,bl/>\2ARG#  8;,pFtsbWh*'S;L/?aREM0Z8+r UJX@ (D Pkl-?(]5 !  1^q$JE#R:)k^ByDNPf prOp] V|{'pnjO>7"[gQ_bpJg^:KB"_Hd~d'W8$d'@sp+g%X ,UF3 X5rtmdp ]8u*bm8!y!0FRcupM85p#/y8cLQ&]]{o:gp s 8 %e=hQO;d'td}EtNy#ae{,6LOCO$}W\! ap~+,;_xdd4:t83n GZ+g#,M".YU+d-(K){'Od81]R b5'?pQpo-s;$lER 32 1~!4 'F* *TUWm&aRRe.gG] 3kOP6npp4%ukJD CxV0 + pl0im^c +#8'lLOFYcCb|NY_ fTuZeOS]BI{YWU]-X`5s<wz ^A KWZ bP<Qb&ww5r0z#Fe^5`e{d=NZ y5$c]% -B} n+?5Hhh.$;;(>ew,=p 7 Q#E45HZ("C5 "vUv<@6&&V ]f]iM] $avno}$,]> HAGGAAGCGCGCGGTGGCCACAGACTTGTAACACATCGAATCTGTCGTAAAAGGTTGCTATTTTTTTGGATCCTAGAGGGCCCCCTATTGGAGCAGCTTAAACATAATTGGCAAGTGTGCACCCCCCAAAGACAGACGTTGCAAAGGCTAGCCGTTAGGTCTAAAAGCCCATGCTGGATTTTCTTCACAGCCCAACTACCCCCCGTGATAAATTATGAGAGAAGGGGCACTCGCAGTGCCGTAGCCAGTTCGTCGTTACTGATATTCATATTGGCCAGCGAGGTTTTGGCAAAAACTTACTCCTTTGTAAAAATTAGTCAGGAAGGAACGTTCTCAAGCGCAAGCCAACACCTTCAATATGTGTCCCCGAACGCTATCCTTTTTTCTGCGGACACAAAATCTTGCCACCTGAGTTGTGAAAAACCAGGGGCTCGATCGCATTCGAGCCGTATTGTTAGCACCGTTATTTTACAGCGATTTGGACCGCGGACACCCGCGGGTGATAGGTTATGACCCCCCGTCATACCACACCGGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:36:10 -04:00AEPt3AEPt3APFN YAPXV2APrN |APrV1APrX}}ɅARTNASPFASPtZASPtZB1PtYB1PtYBCTSOcBufT ICMNT ECTID CTNM&CTOw9CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA 'N"bDATA 'N";DATA 'N"DATA 'N"DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȲEVNT ȾEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~MLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS%OfScXP1AM.; P1RL.6P1WD.?9P2AM.GP2BAKP2RL.CgPBAS(<PBAS&%PCON4PCON2PDMFBPDMF& PLOC..PLOC.*SPSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%%SCAN3SMED SMLtSMPL'SPACAnSPAC&SPACAnSVER4.0SVER OXSVER,,ȆSatdX^Scal@Scan3TUBEB5Tmpr<UserphARphCH& phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN YAPXV2APrN |APrV1APrX}}ɅARTNASPFASPtZASPtZB1PtYB1PtYBCTSOcBufT ICMNT ECTID CTNM&CTOw9CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA 'N"bDATA 'N";DATA 'N"DATA 'N"DCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȲEVNT ȾEVNTEVNTFTab]FVoc]FWO_GATCFeat..]~GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~MLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS%OfScXP1AM.; P1RL.6P1WD.?9P2AM.GP2BAKP2RL.CgPBAS(<PBAS&%PCON4PCON2PDMFBPDMF& PLOC..PLOC.*SPSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%%SCAN3SMED SMLtSMPL'SPACAnSPAC&SPACAnSVER4.0SVER OXSVER,,ȆSatdX^Scal@Scan3TUBEB5Tmpr<UserphARphCH& phDYbigphQLcphTRphTR2I\(f^t e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J]b bhIy.ToFY%NHa0$Cg)QN2P$![_6,? W]H6X+9mvH%lFU?-4$&FkNp&iJ-[qr[&Xj|82Z~9}j~:HzAb9ECQ/}Z&['K2;+hj3ST?0sKG'BNp3JWTX+9_e VO[O~}4}XfSap<#2_<2R57}?hik?]wJUo6X?/WZ#&a:Oc!X|eDNJ{s3x>rb TQ3U'.QR0Iel1}ssb[l"G>=?]p&qqQIxw}H{-*zir8rP p'Ic62nFT16Y=*"+6SPDP-<^g{#:#rT8:BSo?&D|4" ThNW~L?n[Y<,h`\wmj7a;A{%i6#a)lmeudrVLgJRv)1mfc4hApvaUG