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!"+$!*(&(0"-(&($ * &#" #             %(&%GGVk~ytN^A72D<.<*-.!23$'                                                                        d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9}{ywusrQQQHHHfff}}}      )))111999BBAIIIQQQXXW___fefmmmssszzy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b1f72c4b847244789e9dd10e18c90b2aSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-17_00-07_18713130Seq18248138283_K1344_psbafjmadera96-WellPolypodiaceae_K1344_E4_psba3fKB_3730_POP7_BDTv3.mobSeqPop7z /AWr-P|W(W / V  !5TD !P!"M""""K!!!J 7g-e;/q=_<& Z2 G b#; / t  g  P  G|#^'`n _/Y*rXB2*)-376427D\z1Mq] dDs:`: V 7 K 5ya ` ! 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'++%                                                          %0;DHGA8.&  lA \AU2ATAABKB.bcpKB_3730_POP7_BDTv3.mobtermTCACTATCTGTGTCTTAGTGTTGAGCTCCTTCTAGTAAACGGATAGGATAAAAAAACGGATAAACCCGTATGGTTATTCACCAAAACTGGATGCCAAATCAAACCCCCCCCCCCCTCCTGAGGAGGAGGGGGGTTTGGTGTCCAATGAAAGGAAGATCCCTGCAATATAAAGGGTGGAGTGGAAAAGGGCCCACCAGCTTTTCACAATTTCATGATTAACAGTTTATAACCTTGAATTCTGAAAATTATTTGCAATATCCTTCTGCGATTGGATAAATTATGACATTTCCGATTCTATTTTGCGGAATGTTTGTAATCACTATCTGTGTCTTAGTGTTGAGCTCCTTCTAGTAAACGGATAGGATAAAAAAACGGATAAACCCGTATGGTTATTCACCAAAACTGGATGCCAAATCAAACCCCCCCCCCCCTCCTGAGGAGGAGGGGGGTTTGGTGTCCAATGAAAGGAAGATCCCTGCAATATAAAGGGTGGAGTGGAAAAGGGCCCACCAGCTTTTCACAATTTCATGATTAACAGTTTATAACCTTGAATTCTGAAAATTATTTGCAATATCCTTCTGCGATTGGATAAATTATGACATTTCCGATTCTATTTTGCGGAATGTTTGTAA=D^i !.=IVgw '5BLXds "-8CO\jx'4@KWdr !-9EQ]ht)3@LXeq| &1<IUcmy)6CO[fs   " , 7 @ J T d n z   ) 6 D N [ g u ~  " 1 > G S _ j x    ' 1 = J U d n {   " - ; D M \ h o { '4@LXeq|=D^i !.=IVgw '5BLXds "-8CO\jx'4@KWdr !-9EQ]ht)3@LXeq| &1<IUcmy)6CO[fs   " , 7 @ J T d n z   ) 6 D N [ g u ~  " 1 > G S _ j x    ' 1 = J U d n {   " - ; D M \ h o { '4@LXeq|  :5""''" !"#*"!'**&#32&*&&'')  1)3;(++3)"32+)"+(0.331'+&+)+  '(," "3332"   " $" """+    (    "   ""         "         :5""''" !"#*"!'**&#32&*&&'')  1)3;(++3)"32+)"+(0.331'+&+)+  '(," "3332"   " $" """+    (    "   ""         "       2/cRW+'$P&O8c$+#A.W?b" V\[N=RXS A$B 2+HR0a7OLE)53L+JC"#6Rd:J]8%YE7WP2+;\YF-_6/<.9@# W 2A# $U3<-]$:N@d`I=P1J:_*%\!M9^b5XC<1cU< Y9%_"#A /,bCT>^;B V2FDF*8@*OT%K>Y&2B&`MK]?C4_&2>00?Z0C</91)a83?S%EGYI_E2V*'$$ ,P,I2XZ%, M&J `_'+&>J9`& t h X / t C[< OA:sDX4E}"` q9czJgH#b5 S6Bw[GUGr:ipuhGT<xvg/3;>CT,rlE } m{AuC"%"/Rdj bA(s7 +-*tsqtRl\**W&?*SY5 [e|>' a"CNzeON# ]'O/^x `8{ s%FDRoyD_V6F?DcGXhH1*48: $6$)27*s"? 35    :-      !$+ %@$+ I=$  l\ppCyxMHFU,aMcZ^<g#,/A_bj,T _qs}q`~Axdg5SZao5.o89]z fl p8DJl)Ar5kPHQ.uOwKSq"As}Xi U>1dBJC2A""l gn  v  Ds2*e   ?^hP* Sz?+clHr m^Q~[JV"d.`=2_w9fS;\s${fVo 8/x1 Tkt#J'u(">4[muA9T6#9 'Bi9#+_#<j8(l??W;htm#_\?4i4k=9UtLj|;1{$TxXNGGTACAACCGTGGGGGCGAGGTCCGCTTCGTATAATTGAATTATATTATTTTCTTTAATATCTATTTATCCCCCTAAAGGGGTCTAGAAGAAAACTCGAGGGAAAAAGGATGTTCAAAAGAAGTAGTCAAAAGGGCTGAAAGGTCATTGTTCTTTATTACAGGGAGGGCCCCCGCCTTCTATTTGATTGGTCAATAGGAGCGGTTCACCAGAAGAGTTAGTAAATAGGAAGGATTGGTGCCCGTCCTCAACATCACCTAACAACGTTGGTATACGTGCTCATACAAATATGGGTCCAAAACTTATTATCACTACCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 01:37:39 -04:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTS6 BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'NDATA 'N2DATA 'NDATA 'NDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~4LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISP1AM<x)fP1RL<x&P1WD<x+P2AM<x0P2BA<<3FP2RL<x.VPBAS<<JPBAS<<PCON<<%PCON<<$vPDMFPDMFPLOC<x!PLOC<xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACMSPACSPACMSVER4.0SVER 6SVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTR'This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTS6 BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'NDATA 'N2DATA 'NDATA 'NDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDeFVocDTFWO_GATCFeat..D&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~4LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISP1AM<x)fP1RL<x&P1WD<x+P2AM<x0P2BA<<3FP2RL<x.VPBAS<<JPBAS<<PCON<<%PCON<<$vPDMFPDMFPLOC<x!PLOC<xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACMSPACSPACMSVER4.0SVER 6SVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTRITNn%&nJ9."G>H15KK;x^OSvqy4=(Wz`&t$SUf^3i&6j|z[vRP|ZzrA^iaHtV2}2?Yk6]DZSPW~VlWj+8w*<_swW^$oVv+rrbN<6h8b`[.b;$,PCH-p9b6mtTUk1T:29"5-z]T1S6RA0W7yLlo/&U{uclQ[vL}W$P1ObYn#pwW(YJ_93dy5*=gb_nwkI6dNNwM@B8{A)P|p$S!T,lwd-l#fr2i:}WL~?O./bE:#&PV)ob&6O`Z?5t}"^wuCt(3ksKp/(ZU&benN#;98wXu0oPnBT16Y-e+&54bn%&|CO1h,9n5N`!1;ULWyS#|Y;;y+Y3y'EV}sRCkSh!0i9>Yn2Pajeo4]tRi^as>0cPX*F8Bir!3EY$9eKoQD5pYHT"Hf.Q*s`AtI_h!"9+325;(g8G8UT)b&Lotr%3bbcy[*oCf!`LY&0AS_7eaPt;LOx,T2PYbWFl>o 8:T3?qm#vRRC(vJayr=u4ZfoQe=N^tpu&ofW)NKog~!-|r< wvr]LaiY2]kO~JcW`$~~;`.pr$,.[G57'9\47.SbXW,62DmOYX/D?**s$[-HbD:/~sN91`zKA[Lz2fJq3IxdOuYn!OviO"%K-FY({/mpFkp#Ml(8u@w@+V2o~*,p""#]eq }X'KD7I 9_[*?bCDkSOj!Q4M"}JMzR9JK[Yl)d762H#A#Q9U,Z:X=<;q4n%DSi^tpuze2#Xxiy?,1OPh~wA[h@tC]"#]}l~e,SXtmI^#lW4?/D:Q64Pot pN9IlVOY(xs/b@j/.=y`6@{u4f\TP/h;ZQkwqzFGP7T]V%R5;MmN2CgGz^nLoT.iPtj glX ,cW`e;E;qK|-Vz^b;0|>kij7!}WQ`QEJ9."G>}O!:i1At*ldAT/n@6TdO{7+VDSHM6FNmMOx^.9,(B~b4O{[=Ku%9tWa-cZ,\|b=-j%`3EFilpAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtAUDTRxB1PtB1PtBCTS6 BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'NDATA 'N2DATA 'NDATA 'NDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabDeFVocDTFWO_GATCFeat..D&GTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~4LIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOISP1AM<x)fP1RL<x&P1WD<x+P2AM<x0P2BA<<3FP2RL<x.VPBAS<<JPBAS<<PCON<<%PCON<<$vPDMFPDMFPLOC<x!PLOC<xPSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%SCAN3SMED SMLtSMPLuSPACMSPACSPACMSVER4.0SVER 6SVER,,nScal@Scan3TUBEE4Tmpr<UserphARphCH phDYbigphQLcphTRphTR