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"   +                               5                 '(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR ~|zxvtspnPPHHHeee}}}   (('000888@@@HHHPPOWWV^^^edhkkqrqxxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 866bdec22e23466f8024a76fcb67ffc4Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-17_01-37_18723130Seq18249138285_K1345_psbafjmadera96-WellGaga_K1345_G10_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zSVZ`gnrtvzynhdcb`]ZXWWXY[]afkpux||wrmiedba]XRLHGJMQRQMJGGKPV\accb_[VQKFCBCEGFD?9410256752.*(''()(&# !!!$&)**)%"!#" !"!  %'&$$'.8ER_kw ;l )4:7,h>ypkg`WLB;:=BGJJGCACHNUY[[XVUUX[_beffec`^]_cgloooopu}(6CQaswhWF4"ylebdjuN-t=d({i^XX[`dfffhlrz &HsGqk?H gXNIGEEGJOTWXXXXZ]acca_]]`fnv|uniedgq!t47es[ YwbG847@LZj{9gF sQ8(&3Fb;5ML1w*e9  ,<M^n{|lZF5)!     #+9J\jssk^OA6.)%#!"&1GlIKqoF=O|IL& )N VMp<  &nu2sr/#9 $0=KU[ZTJ?626BUl1p)6BXh&O" %Fq  Y. #0:??9/$ Bz@goX&\, .HgpO4  #4FWdnrpgYF3"   J-pw7KFj>"(7DLOLF?830.,*&" !&/;IWckprqpopsx~ !"#%)/6<><5+!o_RHB@??<83,%  #.6:82*"  <bX1 &GkeD( +=LTUOD6( 0Pth@  "DltG  #FioQ3  *Im^>$    7e'nchxmM1 )Ild@    &-269<@DJOTWZ[]`cefgghknsx{}}{xvuvy|ztojfc`]YTOKJNZlsS6$"3S|lC##;UkwwlW>%%AevJ&   7PguyrcN9&  $:O[]SA+7Uo~}mR4 .@OTOA.  %<R`d[J4   !6Nfzt_I4# (:QkjN:3<Qm~_=!'DegH)$=TbdYE. )?R_aYJ7%  $HqiH.   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"%'(&#&/:DMTWVSNIEB?<7/%$-7AHLLG?5*" '042+!#*18?DE@7)  !0@P\cc\OA3'    )2674-$   "5GU]^WK>2("    &(% '7HYfopj^O>/"  "3CPY\ZTLFFM^vyaNB:2)/CS[YOB6//5>IS\dkpqlcXMGHQav~kYK@82,'#"$(,/0/,(&%$$##&,8GWfoqmcXNE@<:740*"1X1Q^XC#ztsttpi_UNLNSWYTI;,!#,49;>ERez~pcUG7)                     K,AA'GA^qA%KB.bcpKB_3730_POP7_BDTv3.mobtermGGGGTGGCGCTTATGGACCTCGTGATAGCTTCTGTCGAGCCCCTTCTATAACGGATAATAGCCGTCTGGTTATGCAGCACAAATGGATACCAAATCCTTCAACTTCGGAAGATGGAGTTTGGTGTAAATTAATAAGGGATGATTCCTACGGACAAGATAACAGAGGGGAGAAAAAATGAATGAAAAATGTAATCCCAACTCCTTCTACCCAAAAGATTGAAAGGGTACAAGCATTTGGAAAATCCGAATGATAACTCTTTCTCAAAGATAAATATCATAAGGATATTCCAACTATTCTTTCTCCCCAATTCAAGGTGGGAAACTAGAGACCGGGAGATTGGGACAGGCCGTTATTCTCCCCTCTTTTTGTTCTACCGTAGGAATCTTCCCTTTTTAGAGGACACCCAACTCCATCTTCCGAATATAAAAGGGGTTGGATCCTGTTGTGCTGCATAACCACACGGCTCTTATCCGTTTATATAAGGGGCTTCGACGGAATCTCAATCTAGGGGGAAGTTATGAACATTACGTTCATGCATAACGAGCGAGGGGTGGCGCTTATGGACCTCGTGATAGCTTCTGTCGAGCCCCTTCTATAACGGATAATAGCCGTCTGGTTATGCAGCACAAATGGATACCAAATCCTTCAACTTCGGAAGATGGAGTTTGGTGTAAATTAATAAGGGATGATTCCTACGGACAAGATAACAGAGGGGAGAAAAAATGAATGAAAAATGTAATCCCAACTCCTTCTACCCAAAAGATTGAAAGGGTACAAGCATTTGGAAAATCCGAATGATAACTCTTTCTCAAAGATAAATATCATAAGGATATTCCAACTATTCTTTCTCCCCAATTCAAGGTGGGAAACTAGAGACCGGGAGATTGGGACAGGCCGTTATTCTCCCCTCTTTTTGTTCTACCGTAGGAATCTTCCCTTTTTAGAGGACACCCAACTCCATCTTCCGAATATAAAAGGGGTTGGATCCTGTTGTGCTGCATAACCACACGGCTCTTATCCGTTTATATAAGGGGCTTCGACGGAATCTCAATCTAGGGGGAAGTTATGAACATTACGTTCATGCATAACGAGCGA(@KWhw  6NZgn!2>JU^hv *4BN[gv'1<HVap !+6CQ]hw.8EPZer~%1<IS_kz !,8ES]kv   * 6 B N Z f q }    & 1 = H T ` k x    * 4 A M X e r     ' 1 = H R ` k y   * 5 @ L Y f q }  "/;GS`lx'3@L[dq}*3ALWdq{%1=JWdoz*7EPZhs)3?IV`ly #-:GR_jv+8CP\hs)5ALXcp| %1=IVbk{ "-9ER_kw *5BPZdp} (@KWhw  6NZgn!2>JU^hv *4BN[gv'1<HVap !+6CQ]hw.8EPZer~%1<IS_kz !,8ES]kv   * 6 B N Z f q }    & 1 = H T ` k x    * 4 A M X e r     ' 1 = H R ` k y   * 5 @ L Y f q }  "/;GS`lx'3@L[dq}*3ALWdq{%1=JWdoz*7EPZhs)3?IV`ly #-:GR_jv+8CP\hs)5ALXcp| %1=IVbk{ "-9ER_kw *5BPZdp}   (       "0!-)!#                                +"         '2                 "    "'                 )   (       "0!-)!#                                +"         '2                 "    "'                 ) GWM!.S"":a R+XI`%)X"-4"`JTZD'93*I:/1DZHLJLG^LC2W@X/ 0.>@L M]@T`Ub2=YCTa! >[I?A_([@C :6_<3D@I<_$X( 3*H^'VK  P.2ZF9 U_-G:;;PIP R.@VV \H;S 4WI&< /& aM;U-HR+?1U]5+3LV^>",( 1;I\$>RYbS[*\ 1B^U WE 4<dKO 6=:B,VI81+ Yc IT3[ 5 .b$'Q DY6)cA $1U< O:+$PT8 Q*F&"8RDD$4HA5Z`XO= *5--44,NAN!'A?U?b EHUH1!^O9W#H). EAFYX/-Q J6E)C# 3(O []` TL %F=KKV0D 64=X,,ROb<B6EUa'R`C6 Y]<C!)2`35.`9 OO W W&FFE` $` J_(-O.2BJEA0K$M`a=iO: 2.((n(];1ggkRUIif6Oc^;f 0 _ 'z\;AOkTv G Am %V>do*"S>X}foc4L-, B lS?-TV~>A%T^ct&h ZHjGAli`xD(JLT $ T]0CDU%+>!c\:MxM{L%P j0-rWqtqJ,>*W{=AV7`Q~R{=E9#;/YZ6p_5#N%jV^*[XXeY*abaX 7d"4r Vvb)j P "{%RCFT) %[)]]]>vC7x:4{? t$1rMhe<Bh#sRL'w8 v ,b ag,tk-U (5wIe^~$cXM}HA>8;XEC>@:*+"3 6RE[%&`w{G4U0FQvKhK]=%(} BpCc%5WI BI K%-L+ix(U55l 7   %+  =*91D; K2:!DB<L!1 @O W)Y X&u1B$c)Rq.5AW5$- q (/>Iod/ n)F2?:p)'14298;/ 3@"J#0.-LI+C.(N6?&?5R;=7&g'b16E.]3pe_D!kLn@KK0K(K009999N9_Ad!A(006- 0SeOZ99999>79KK0K500Z0KK0ccDWA0K9A0K00Ac0AO0pOppJpY!Q1-}jvIizbYE@W%OeOl:\@oxfZ6pIp'e)D{3,=B64*DyUK;r%k3_B pSpV^Agh0NY+ [eh  z2I^xmOOV5F/K f+wy"8]MG7?CsS*9f4WCK a_FX\-4z OY_o;z=_YqWI-_dK3e@mebhu`_}94kbQIb< H^RD/ wIBO]dD_Cj rIsMqD(n9<ucV!j. (2 AAATGCCACTCGGGAACAAATCAACATATCCTGTCTCCAGTTTGGTATATGATATATTATCTTAAGGTCACCCCGGAAGGTCCCCCTATAAGGCCTTCAATCAAAAAAGGTTCTTCCAAATACCCCCCCACGAGTTAACACCAAGGATTAATAGGACATTACAGAAATCTGTTGCGGTCCAATCCCCATCTCATGGTCACTTCCTCCAAACCTTAGACTGGGAATATAGCATCGCCACCGTTATGTTCAAGATTTCAACCTCGGGGATGTGTATCTCGTCCCGATCGTTTGAAGCCTGGAACTTTTTCAATCTTTGAAATGGAAGATATTGCTATACCATTTTTTTTTATGATACACATTTCTCGGAAACCTCGTTAGTTCCTGGCATTTAAAAGGATTCTGTATACCTCGACGAACTTATCGTGGTTGAATTAGTTTGTTATCGCACACTTGAGGAGCGCTTTACAACTATTTAACTATGTGCAAAGGGGCCGAACCGAGACGAGGGAATCAGGAGAGGTGGTTGAGATCCTCCCATGCCAACAAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 03:09:22 -04:00AEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtKASPtKB1PtJB1PtJBCTSDBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &M.DATA &M.2 DATA &M.8DATA &M.fDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~B{LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AM$H/7P1RL$H*P1WD$H3P2AM$H<P2BA$$@WP2RL$H7PBAS$$PBAS$$PCON$$(PCON$$&PDMFPDMFPLOC$H"_PLOC$HPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %9RUNT RUNT RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAq WSPACSPACAq WSVER4.0SVER CSVER,,Scal@Scan3TUBEG10Tmpr<UserphARphCHphDYbigphQLcphTRphTR5KThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ŒAPrV1APrX}}•ARTNASPFASPtKASPtKB1PtJB1PtJBCTSDBufT'NYCMNT UCTID0CTNM6CTOwICTTL LCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'N^DATA'NDATA'NDATA'NHDATA &M.DATA &M.2 DATA &M.8DATA &M.fDCHTDSamDySN -Dye#DyeN8DyeN=DyeNBDyeNGDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabR^FVocRMFWO_GATCFeat..RGTyp!! HCFGCEHCFGHCFGHCFG==InScInVtLANEDLAST~~B{LIMS!!bLNTD2LsrPaMCHNMODF ƒMODL3730NAVGNLNE`NOISP1AM$H/7P1RL$H*P1WD$H3P2AM$H<P2BA$$@WP2RL$H7PBAS$$PBAS$$PCON$$(PCON$$&PDMFPDMFPLOC$H"_PLOC$HPSZE`PTYPQPXLBRGNm(RMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT %9RUNT RUNT RUNT  Rate RunN""S/N%SCAN3SMED SMLtSMPLSPACAq WSPACSPACAq WSVER4.0SVER CSVER,,Scal@Scan3TUBEG10Tmpr<UserphARphCHphDYbigphQLcphTRphTR,9drWdG| nG\|8 f&hPVqzgN$6`a'K-n?5uHAId4|Cn=aib*y%`;5m;*/P9m4%2pQWi?owO9KfPZ2V~oL`H9R/+3p+M*JgbUA>kij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9Hb?j !jCW8066X?J`GVR_6MW!w3[s2G[=ih2[-?Si#P6[s$$K%v'nE918sIRD8z{S>!lXS:Q&:C!g61CfFc':Z^mDt4)Mz9^#m}ml+peZR0\}'L-gY,5^KfoQP-2ZE<}g6M>PzMWhc'kp?_sJOhoN^U`dbM;Y@iGu2tG=}V7V=y`6@NBF{l9cGY@ZWeoazNpP S!oS_,y".9+5SGKI'_26tKR"\3|>oW~!Eb4;,~sD[V$! K]T5^.?s|"Ff61}XTu.ycI0 rJyKq6:gtPMupi4Bd $Di~&|m&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k_l_e5ll@i2zeqr[&X0VDE?g`pMCW8>6DSN{4;NJlHQoQ8p[UR6? sI(J8dR9F![pE)}%z5dJ6vvdeW\j//u4aO/CsINi@C)Re/K)Z6AI8rBAr] {[q?Eb,f=E]rRS$~=}F)h>S]p?_sDkrD5pYlGcRbi`)lZFGz/X<7:?w5RiV1]cs:*?FvHQnS[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0