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"                       "           !                       ! !       %                    #    "            %      #    $                               % "                                                    "           )                                                      "    #       '      (  $                                    #   "                                                       %                      #                                                                                               $                   &                                      n,,,III^^g{{&&&999DDDNXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRG~||yxvtrqnmkih;OP HHHeee|||   '''///777@??GGGOOOUVV]]_ddjkkqqqxxx}}}a !!!""""###$$$%%%%&&&''''(((()))****++++,,,,----.....////00001111122223333344444555556666677777888888999999999::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 024fce57ae7d49368b30ec40ab4c68f8Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-17_21-04_18733130Seq18250138295_K1346_psba3fjmadera96-WellAdiantum_K1346_H1_psba3fKB_3730_POP7_BDTv3.mobSeqPop7z?@ACFJOSVZ^bfjntz  8Sl)Hl8Vn|}rbQE?@DHIC5|oaRE;40+"tkbUD1~usx?`{{Y2 '2;BFF@4! 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"*-*#  $&#  3FT^a`YO@0   #%%%#!      #&'&" )166/$!    #*032,"  -Jew|taH2$"/GenQ5 %&!$:XytP-7YyhK1 "'*)&!"&)*'#)132/09I_vnT:% %A_{|l\PG?82,'##),*%    $&$           !''"    !#%%#"    #&%                                                                                                                                           Dt@-A|A=A<6KB.bcpKB_3730_POP7_BDTv3.mobtermCCTAGGTCCTCTAGATTAGCAGCGTGTTGAGCTCCTGTTGTTAATGGCTAATATTTATTAGAACAATATATCTTTTAAATCAGTCTAATAAAAAAAACTAGAAATGACCTTTAAGACATATATAAATGTCTTAAAGGTCGTTTATAACAAATTATTTATTATCGGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGCGCGAGATCCTAGGTCCTCTAGATTAGCAGCGTGTTGAGCTCCTGTTGTTAATGGCTAATATTTATTAGAACAATATATCTTTTAAATCAGTCTAATAAAAAAAACTAGAAATGACCTTTAAGACATATATAAATGTCTTAAAGGTCGTTTATAACAAATTATTTATTATCGGGCGGACGTAGCCAAGTGGATTAAGGCAGTGGATTGTGAATCCACCATGCGCGAGAT)8>GXcj|#.>KWkz"2@LXcny ,7CPZhu*6AMXdp{  -:GR^iv &2=IT`ny*5AMZeq~)4@LWdp|  $ 1 > H U a l y    + 3 = G P)8>GXcj|#.>KWkz"2@LXcny ,7CPZhu*6AMXdp{  -:GR^iv &2=IT`ny*5AMZeq~)4@LWdp|  $ 1 > H U a l y    + 3 = G P    )224*/#.2##.!! '*!**'',,**38*3,;88))  ""'-3--8;833+3113%.++'"+""1;;';3"""$1)+1331)"++"""++"','')"'-"+-+))3"+3")""" ##3 ###'**      )224*/#.2##.!! '*!**'',,**38*3,;88))  ""'-3--8;833+3113%.++'"+""1;;';3"""$1)+1331)"++"""++"','')"'-"+-+))3"+3")""" ##3 ###'**  P"LT&M M,7# Q YI6RM/2"F'N 1( 2 X<9 " L)3'4 V7`BR;XbHJCMc3YASOC Y*$-'1DF29aD5'51bM<DG)24A #+Y0NQ ,! L@S RF3M;FO` NV4)8c6J!W@Tc+#,d?M <L`'JR9L2\_]X*^$.NH ]%-:*@A: 8 SR6F',<Q^@OE\;1:c ; YN SH P/W7Z<Bk=) :^1il[-jy,z^q0) xpH14 Nz`]f)@g/`_ozDBp6F^[l{nFPN+Tx!w9^S(7xw /h0q*BW3zn#[ghWRXBT+CRX;Re6B?S8M$"#b63W@EG2T7  -7 %CU0 0)+  M N&&  6  ! ! #$$0)*W!&(O/K0 Hppp4,pd7fh#}*8pwi(7 p)pdCTdT,Pzf3Afd01Bd,8+P,6cC;_UdN$x`6,#~;KW&Fp@^=OnOg@W0&nXgrw $V ,:,>gTVXoOZF^ZQEtoe,.i=. Vd2oOv:?AB(=ma0bQq!D&2VV"B0gL_>GYAAAGAAATTCTATATAATCGGCGCGTGGTCAGCTACTCAAGATTGTAAATTGCCGCCCCTCCGACGCGCTAGAAGCCCCCTTAGGGGGGGCCCCGCTACTCTTGATTTGGAATTCCTGCCCTATGGATGGCCTGGACAATCAATAGGTTCCAACAAGGGGTGTACAAATGGCCCTAACCTGACCAGGGCATTTCAACCCTGTCCATACTTGCCTAAAGCTABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 22:52:30 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSNBufT CMNT ȝCTIDxCTNM~CTOwCTTL ȔCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA >DATA DATA DATA "EZDATA "EZ2DATA "EZwoDATA "EZDCHTDSamDySN uDye#DyeNȀDyeNȅDyeNȊDyeNȏDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FWO_GATCGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ȪLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS+P1AM P1RL FP1WD P2AM.P2BAP2RLtPBAS;PBAS^PCONiPCONPDMFPDMFBPLOCPLOCPSZE`PTYPPXLBRGNmpRMXV1RMdN ZRMdV1RMdXcRPrN>RPrV1RUND RUND RUND RUND RUNT :RUNT 4RUNT  0RUNT  69Rate HRunN""NS/N%#SCAN3SMED SMLtSMPLSPACMSPAC;SPACMSVER4.0SVER CSVER,,Scal@Scan3TUBEH1Tmpr<UserphARphCHYphDYbigphQLcphTRphTRفThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtB1PtB1PtBCTSNBufT CMNT ȝCTIDxCTNM~CTOwCTTL ȔCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA >DATA DATA DATA "EZDATA "EZ2DATA "EZwoDATA "EZDCHTDSamDySN uDye#DyeNȀDyeNȅDyeNȊDyeNȏDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTab"FVoc"FWO_GATCFeat.."iGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ȪLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS+P1AM P1RL FP1WD P2AM.P2BAP2RLtPBAS;PBAS^PCONiPCONPDMFPDMFBPLOCPLOCPSZE`PTYPPXLBRGNmpRMXV1RMdN ZRMdV1RMdXcRPrN>RPrV1RUND RUND RUND RUND RUNT :RUNT 4RUNT  0RUNT  69Rate HRunN""NS/N%#SCAN3SMED SMLtSMPLSPACMSPAC;SPACMSVER4.0SVER CSVER,,Scal@Scan3TUBEH1Tmpr<UserphARphCHYphDYbigphQLcphTRphTR,qJ'8f7}7a2&d:Dp$^HA.tHZKo][571\hNQ0J;9 |!$!o%QG{c!%88 =![z)+^[,MZ@Y+P'#:&iChk}`:r&^?HYTw|bT|MI>"J268[`#|D5n4]p%!5r#W3}hxv_1=|:.\_'gle#Q\WWs:rGbj8W+Sue]^WOSLj$7*pki/CF"B``,#Xr_<.^ioA+7x,"L-2QvA-zI?I,z|>[mLoCDaD/|8i.bb>~ n3]NE^O{Nf7{hAh.iOWhbk1^wK[*oCf!`LY&'!"lC+(Ci5T0U3{T4G|1l UNM(T[ S]T'_C:j|(5,fWz"$b[8)FTk2X'([U)P? s>}J8kOAWm ;op$SqwgE6FM;v&KT_ZO~|Q5_e-1^H/wwBbFK#giOA2|YU'QcWVQ}QH8EE.Om,K]yDWYhY8|kN_uw >xPo1Cd}Fp82)XaM~VpYow.mU5s%<(]R06`NV-[?|$}~?V'i~?RVuUi\j-=p\q_W-'Ic6XH[AVePX!Hiwe2{sGiSVqrj/.3]HCi%{1k :O[O~}<"&eG1VcyST/4f_7}?e_MghW~^(';_/C]2ZE<}g6M>P:Y'((nIcqq2t>1j.\H!Ud?Ft/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuUIcTA?i]8iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>S]T'_C:q|4G,fcp,@B_8Es{jGyZ.E/zC&/VnZAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtASPtAUDT0B1PtB1PtBCTSNBufT CMNT ȝCTIDxCTNM~CTOwCTTL ȔCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA >DATA DATA DATA "EZDATA "EZ2DATA "EZwoDATA "EZDCHTDSamDySN uDye#DyeNȀDyeNȅDyeNȊDyeNȏDyeWDyeW8DyeWSDyeWgEPVt!4EVNT  EVNT EVNT"EVNT5FTab"FVoc"FWO_GATCFeat.."iGTyp!!THCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~LIMS!!ȪLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS+P1AM P1RL FP1WD P2AM.P2BAP2RLtPBAS;PBAS^PCONiPCONPDMFPDMFBPLOCPLOCPSZE`PTYPPXLBRGNmpRMXV1RMdN ZRMdV1RMdXcRPrN>RPrV1RUND RUND RUND RUND RUNT :RUNT 4RUNT  0RUNT  69Rate HRunN""NS/N%#SCAN3SMED SMLtSMPLSPACMSPAC;SPACMSVER4.0SVER CSVER,,Scal@Scan3TUBEH1Tmpr<UserphARphCHYphDYbigphQLcphTRphTR