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C h  x A VNFW3nTdo=(rkb[LAx~{}htnlNMWAJDP4S9H<7QB><VJa`ipm_\J_ZOPC>:/$-6(&-)/0;!7EHUCGW5<.1.C/)+2" $"5&%*)$$, " "#   1 1.@NR;-2:*)     2%*   %601$  0'&1%  +94;>.!3  9TEtunlbH;# ##     !+*FG88V1'  3+CHfV3C!6>R2I5)0&&  '%41-1,+  "7N-338%#",B=[dWNdlGVACA(  !"  *U>kmt]OZBC 2<UU^al`FsHONPD,E(%$ (  3U[bXgj^O\ZEB#$A*,FELG:! )9<<XYifiTK^F9' !PUYHGN10# ,7=1D/:,&%8#0)7F@=+A'#0    H<KFchTXN>M<#&25&+.3HC.! ) # +#"!/,5%4=).( +7'BCXS[PG^n:3?pN7$%89$W@@)2! wcSH zbcSJQI6 .'&*$     #', 741 '   ,D$ <?N3=<8   /91=JDMMckkfa<7<" "("-# )-3CF94%    1&4>KR9D1-  (:750--6SI_RXObLF=(7#    /4*.5++  25GLTHC>),#9,% 2         5&3&"6*.+#"&(#4,B36)@$#")#)!@!"-"&   6Y,,,III^^^{{{&&&999DDDXXXaaavvv      ***333>GGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR2~}zywtrqonPPPHHHeee}||   '''0//877@@?GGGOOOVVV]^]dddkkkqqqxx{}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: b10d19dd274e4445b7c8bccfcfc73469Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_22-36_18703130Seq18247138284_K1345_trnhjmadera96-Well&Myriopteris_myriophylla_K1345_C5_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z'(*-3:AGKOTZbkt} .ATgz.=JUalv}rdSB0mU@- wpjgfeeedddegikmnoooonmjgc_ZVRNIE@<730.,+*(&$#####"! #(-4;CMXcqo_QF=61.,++.3<IZn%d]$=d^Co=|rh_XQLGC?<:8889;=?BEHJJIGHMXizpkijkkhc\TMF>70)""&(**)(&%$"!   &0=JXftysnljkklmljf_WMC91)#  %+29@FKORSROIB90'  "-:GS^ehgaXM@4)                         "$$# !#$$##"! !"#%')**)(&$#"!    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"%(+.258<?BEGJMQUY]aehjmquz  ueWLC=840-*(&%$$$$$$#"! !#%(,049<@BDEEEDCB@=;740-)&#!!%*/6?IUcs)@\~EPN/`lcRD<:93'|=lF0V?546=HXlwcO=- $(+-.-,+)&"wi^TLE@<9641/-+)'&$#" "',3;DMXajrvxwsmf_XSOMMOSX_gov{|yri^SI@81+'$" %.7ALV_glopppprrqng\PC7-& $,7CP\elonlhdaaadfijkkkkllljgb\TKC<62.,)'$!!""!  %+/2442/+&                  "&*/4;DNZft!)06:<:60(yk_SI@940-*(%"           "'-28>DKRY_ejorvy|{wrmhb\VPJD>83-(#   @AYAX`AkKB.bcpKB_3730_POP7_BDTv3.mobtermTAGTGCGTTCGCGTATCTCTCCTGGCTGTCCGCCTTTCTGATTTTTTTTTTATGACTTTCTTGGTCTTTATTTTTTAAAAGGTAAGGGCATTATTTTTTTGACGGGATCGATTTTAAATAGGATTATAATAAGAGATATAATACTAAGTTATAGACCGTTGTATTCCCGACCCTCCCGCAAAATACATCTCAAAGGATATGAGTTAACAGCGCATTCAAGGAAACAATTAGTGCGTTCGCGTATCTCTCCTGGCTGTCCGCCTTTCTGATTTTTTTTTTATGACTTTCTTGGTCTTTATTTTTTAAAAGGTAAGGGCATTATTTTTTTGACGGGATCGATTTTAAATAGGATTATAATAAGAGATATAATACTAAGTTATAGACCGTTGTATTCCCGACCCTCCCGCAAAATACATCTCAAAGGATATGAGTTAACAGCGCATTCAAGGAAACAAT0J^x $*8GRdx}$2?HTao~ %1>R^mz,8BNXdoy %3=JUbkw"-8ENYdt$.=HPZiv $2@LWcny   " , 5 B N [ i q }    , ; C P Z d l u  ! - = L0J^x $*8GRdx}$2?HTao~ %1>R^mz,8BNXdoy %3=JUbkw"-8ENYdt$.=HPZiv $2@LWcny   " , 5 B N [ i q }    , ; C P Z d l u  ! - = L       #                                  +         #                                  +  'PQPE'FWC [IT=EQ! aS?*Y *_?G!=G;XMG'3dL5W]S,B[_#8dT.JASRW\PN.<J `2 $ &)6.Z,H`S?F SUV^^_[ Sa.;[A D^aZ B 8 ?\4*#`#5[ ]G1RTN ?83^'8[U8,CCT XW4T0?O5,T22V$0<,d, KHWQ18,8R2 'e$*)s18{8 Zf`Z:Vf}aqR[9]XjGg%C6%F  ').Pz`Ww \ 1bs2 PJLs<oX&Zy0D(y@P?1k &$d~5&P8 Sy)tba ` 62e wwk- UAo ;r%[?qfD!=v_j'RZ+ "G 's^) *1 C U_':97CtUNy #( '7,+)N p88(~x;hy=+3>J6p3j}0=,| ,|+&p?<IwO!Cv65,qkKxe`P|hwOX848Y|oj, pcT^Hk--}<3g%% L"_mdlR{  ,5N$;HX %YR:^+-1"s]+hVn{@5!+_4fm79'j!} SMqg5S@QPzGF:KdgAE5pU7x-sa lq }=ARa[|a?n2jd*NRAGAGCGCCGGTGAGCCAAAAAAGTTTCCCTGCGACAAAAATAAAGAGGCCATAACGGACAAAAAATAGATGCCCCCCTGCAAGGGACCTCAGTGCCAGCGTTGCTTTCATCCCCCCTGAGAATAAGATTGGTTTTGGTCCTATACCCCAAGGCAGTAAACTCCGAAAAAGGATATATTGCGGCACGCCTCATGGAAGGGATTTCGTCGGTTAAGGAAGTACGTCAGTGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 00:07:11 -04:00AEPt3AEPt3APFN tAPXV2APrN APrV1APrX}}%ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS-BufT'NCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'N<DATA'NDATA'NDATA #G}DATA #G,DATA #Gt}DATA #GDCHTDSamDySN ½Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT REVNT ^EVNTjEVNT}FWO_GATCGTyp!!œHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfScHP1AM P1RL P1WDhP2AMP2BAP2RL0PBASPBASPCON PCON PDMF]PDMFPLOCHPLOCPSZE`PTYPPXLBRGNmܸRMXV1RMdN ԢRMdV1RMdXԫRPrN܆RPrV1RUND RUND RUND RUND RUNT $RUNT RUNT   RUNT  Rate RunN""ܖS/N%}SCAN3SMED SMLtSMPL''6SPACMSPACSPACMSVER4.0SVER "SVER,,&SatdH^Scal@Scan3TUBED5Tmpr<UserphARphCHphDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN tAPXV2APrN APrV1APrX}}%ARTNASPFASPt4ASPt4B1Pt3B1Pt3BCTS-BufT'NCMNT CTIDCTNMCTOwCTTL CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA'NDATA'N<DATA'NDATA'NDATA #G}DATA #G,DATA #Gt}DATA #GDCHTDSamDySN ½Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT REVNT ^EVNTjEVNT}FTab$FVoc$vFWO_GATCFeat..$HGTyp!!œHCFGCEHCFGHCFGHCFG==InScInVtLANE)LAST~~LIMS!!LNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOISOfScHP1AM P1RL P1WDhP2AMP2BAP2RL0PBASPBASPCON PCON PDMF]PDMFPLOCHPLOCPSZE`PTYPPXLBRGNmܸRMXV1RMdN ԢRMdV1RMdXԫRPrN܆RPrV1RUND RUND RUND RUND RUNT $RUNT RUNT   RUNT  Rate RunN""ܖS/N%}SCAN3SMED SMLtSMPL''6SPACMSPACSPACMSVER4.0SVER "SVER,,&SatdH^Scal@Scan3TUBED5Tmpr<UserphARphCHphDYbigphQLcphTRphTR4{,|Qv%F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBImE#5!y|\RmdTllvml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^M:W&yY`^Z]}Y?i8O]UBvv{j~(H(I83Cm:wbPn%PR,Qub^ucF=W.X}=}F)h>S[jWcgiQF4Np3<("Hf.Q:F]!wYBTd.u?zD6~hlwXs9?Ou4[42g|5?TK_LLK;6R&^.aiw`6wPjgvG9 HtWo0oW~!Te4;,: TNc:o?G3bmV\7z+M@AKR;p$.jc_T3 #pJE,#VuU,YTl_u4TYe0tpJh&j43ofR5*6Y?Qs?]t~1{[q?Eb,T2|5iZFod>7:I12mxXYnuPQh0{!X<(&}J39OQJM~6Hrd+v/gg61CM#Y\VD*p;I:/wEkfptir"8|oTq}A|1D@<7>{AXG9{$mXCQthxUZeL0:#GqKd[6! x&a"XuF3~h'Wzzqto25+|:23'ngg61CM#Y\V&=o?eU:g;TaywKB_,qz7z}yaih?]mnjH[F.LGZ!.syz4[!.}o|[E^j<(+O}L6>U{mwY':8 v/y#