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A"&# ! !.-!# +(-! !$,!")" /$1 && 0 &d,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: d8ce7ec2456149318a28cbccf41c5474Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellSelaginella_K1344_A3_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zknry$6I^u.](c:H n6a * N  H h | s Q  $ d IeY^wHqks@i  06/mF"\Hukea_^`eny~lW@*wngddfijjifca^[WRMHFFGIKKJGDBABDFILPV_jw7_ 8g{R(}fSF@CRmm~@0N oX\knL5.V  X Z"Gg"eH-xbN<-!&9Pj %Ca~,73yR/ mL3"%'# |pe\TLFA?@EMYi|G"?ORMB5'  gO7wl`TIA;88=FWp$=P[_\Q>$~vttttttuw{vj_WRT]n.WuhAfVNNQVZ^``^[UQOR[j{xj[L>2+(*/4774,#    &@\vvfWI=4/-+)%   2EZlzyjV@*  /DZq @YebN,pJ-  )?YtydRFCHVh|x`F.'Abl[J:+   )4>FLMKD:/#  +4<BFJLKHB:0%   %+169:82*!  *:GOOH<.    &))&   ',..+&     (6FV`d]N:% 7Ts}dL6%   *@ZsvaM>52110.,)(),/256778:<=>><:753100027@NaxgO;-%"   (-.+&!!(3@LUYXRH;-   +AVfopj`TIBBHS`ltsiXA*  (2=FPX^bc`ZQF<2,*-3;CHID<2'    *P|X5   #2@IKF;.$ %0>INLC5'  (4>B@7*   -?NVTK;*(:N_geYF0 "4DORL?."6IW^\SE4#  .H_pwvm`RD7)!5IX_\O>,*<Oamsrk`TH>7438DWshH/   />ILIA6,#  /APWVL<*  !$!  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(4::3( '*)&  $0:??7, (3<@?;4-(%#"!  %,1331.+'#  '-/-'(;OcpupdS@.  %.33-% $*-,("  $08;80& ,:ELMG=0"  (,,(!"),*#  +5993,%"#&*+(!+8??8-   %&$  !-474,#   "! *;HQSND7*  "&'&!     $%"#,3761*!   $$      !*01/)" %/7:82(  #/;DHHC<2(    "!  !!%*--)"     "(*)% #&'%"      !    #$"#%$!   %)*'"   !&*-.,)#    #(,.//,'     #&')-17>DJMMIB90)$""""!         !"!                                                                      +@2AAQیA]dKB.bcpKB_3730_POP7_BDTv3.mobtermTAGTGCGCTTTGCATATAGCACGTGGAGTCGCGTCTCTTCGCCGCTGCTGGTACATCAAATTACAATATTATCTCCCCCCGAAAGAAAGGAGATAATGACCTTGAACTATGAACGACGAAGGCTCGTCCTTCAAAGGCCCATTACTACAACCCGACTGGGACCCCAGTCGAAAATCGTTGTGCCTGTTTTAGAATAATTGAATGCTAACTCGTGATGCTTCGGCATCTACTAACGATCCCACTATTGCATGCTAGTTAGCCATTTAGAGAAGGAACTTCAACGTAATCCTTATCTAGAGGGAAGTTGTTAGCTTGTCTTTCATGCATAACTAGCATTGAAGACATAGAATTGTATACTCGTAATAGAGGATACATTAAAGAATGCTCGTATTATTCCCATTTATCTACTAGAATCGAATCCGCACTTTTTTTTAACCCTTGAAGGGTTGGTACTTGAATACACTGCATAACCGGATAGATATTATCCGTTTTCAGAAGGAGCTAGTGCGCTTTGCATATAGCACGTGGAGTCGCGTCTCTTCGCCGCTGCTGGTACATCAAATTACAATATTATCTCCCCCCGAAAGAAAGGAGATAATGACCTTGAACTATGAACGACGAAGGCTCGTCCTTCAAAGGCCCATTACTACAACCCGACTGGGACCCCAGTCGAAAATCGTTGTGCCTGTTTTAGAATAATTGAATGCTAACTCGTGATGCTTCGGCATCTACTAACGATCCCACTATTGCATGCTAGTTAGCCATTTAGAGAAGGAACTTCAACGTAATCCTTATCTAGAGGGAAGTTGTTAGCTTGTCTTTCATGCATAACTAGCATTGAAGACATAGAATTGTATACTCGTAATAGAGGATACATTAAAGAATGCTCGTATTATTCCCATTTATCTACTAGAATCGAATCCGCACTTTTTTTTAACCCTTGAAGGGTTGGTACTTGAATACACTGCATAACCGGATAGATATTATCCGTTTTCAGAAGGAGC7Kdt'-FOUlz '7@MTam '09MXcnx!-<JS`lw%19CR_it &0<JU]mx)5AKVdpy #/>IS^jt    * 6 A N W c o z  " . : F S ^ i u    * 3 @ M [ e q |  ! - 9 G T ^ j v    * 7 C O [ g s  '3@KWbn{ $1=HVao{")4=FR_ly &1<IU_my #-:ALUdr{ &1>HUaoz '0>JWdqz$2@MZfr}&6@NXgt.8EP_hu(2>I7Kdt'-FOUlz '7@MTam '09MXcnx!-<JS`lw%19CR_it &0<JU]mx)5AKVdpy #/>IS^jt    * 6 A N W c o z  " . : F S ^ i u    * 3 @ M [ e q |  ! - 9 G T ^ j v    * 7 C O [ g s  '3@KWbn{ $1=HVao{")4=FR_ly &1<IU_my #-:ALUdr{ &1>HUaoz '0>JWdqz$2@MZfr}&6@NXgt.8EP_hu(2>I                      '           " '             "        ""    "+"&$)            $ . 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N'iaZO *  Qh)+<9r,6+q:!LJ;yY9YTi7 >w+5S| `z/w?qoV}-?!#y&?<:dNq,+" $`,9IDOfMR,HM;Rp)-Td}8 l*(p`c<-,DjOSMjE-$],pYs9y]>Gws8ZGUQD9d%U1(p i[3V!+1+9J6"A2sp  kRq J b$U1 iSt <^kQSP8e/' ph%gp|Ied 5@Qea,cu-Y*W&" 8]j%8t&Q^hSP,11kTN"M8B[3 (>uKh8{R8)!8 @B| * fn*C?&b_ ;5-NI_M zF7V[D(c*R#3tZ#9,-zu#G 34#ca}j3=?:V;fUb$Nn1G$;-@MeE)o'SpK+ 5y3!I4_T BEOJ}!vWl.`}D9'hm/~~Ee5MyUqxF~a(:}pV+%]-'/\V+\,~+ ?5dBz34g+IN(*<Rx diBHwDE.){-&l VA8emua=^VQ+ts<EAGAGCGCGCCGTGGGCCCAATTCGTCCCATTGCCTCTCCATAGAAGAAGCAATATAGCTGACCGTTAGGATATCTTTGTAAGTCCCGTCATATGTTCATAAAAAGTAGGGCTGACCTCGGAAAGATCGACGGTTTTTGTATCCTAGCTGCATAATTGAAATAGGGGTAGTCTGCCGCGAAAAAAATAACCTCCCCTCGGAGTAAACCTTCTCCTCACGGATATTGAAATAATGAATAAAATTGGCATGGGCACCACCCAATGACCCATAGTACGGTGAGGCAAGCTGTAAGTATCTATCTTTGAACTGGTCTAATAAGCCTTAAGCATCAATAAGGGAGGACACCTTCCAGCGGATACTCATCACAGATTCTACAAGGGTTTATGCTAATCGCAATGGCGCGTGTCTTCCCCTGTTGGATGAGCTCAAGGACCTTTTTCGTGCACTGGTAGGACCTGTACACACTTCGGTGGAAGGGAGATAACAATCCCCATGTTTAAGATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:35:57 -04:00AEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtVASPtVB1PtUB1PtUBCTSSBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA 'ODATA 'O<DATA 'OZDATA 'O.DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FWO_GATCGTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~QLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS, P1AM?P1RL;P1WDCP2AMKP2BAOP2RLGPBAS.3PBAS,=PCON9PCON8PDMFPDMF,!PLOC4PLOC0)PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate @RunN""FS/N%,SCAN3SMED SMLtSMPLwSPACAp>SPAC,SPACAp>SVER4.0SVER RSVER,,Scal@Scan3TUBEA3Tmpr<UserphARphCH,8phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtVASPtVB1PtUB1PtUBCTSSBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA 'ODATA 'O<DATA 'OZDATA 'O.DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTababFVocaQFWO_GATCFeat..a#GTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~QLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS, P1AM?P1RL;P1WDCP2AMKP2BAOP2RLGPBAS.3PBAS,=PCON9PCON8PDMFPDMF,!PLOC4PLOC0)PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate @RunN""FS/N%,SCAN3SMED SMLtSMPLwSPACAp>SPAC,SPACAp>SVER4.0SVER RSVER,,Scal@Scan3TUBEA3Tmpr<UserphARphCH,8phDYbigphQLcphTRphTRMKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@:g-^!rB-%cwpZHssK7o_Kz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAlI{_*.9nWFa-st2hSXdF#lQ%cy-*7y 4/LOfP=6p"i5_+W+V)uOs|w}x?@1m-(bhkdr&1ZG|JWm;2FVc.O_l;C[;6&`_M%_|_n?_;?L-&]S9Hgn_RJ4ir$G%1` b&}3672tX.z=@e?xpL#H~?p M;_8Es{sMV^@}OPDsvA\Fu*tnPl'Z9dgTiK]5bf+mb097KA.M}U+yV\@>fj4ov.lN"@>oW~!Te4;,}aD[h_R9wQi:A,b?Az{fzVS:3AG}X`AL:2ZE?RVuUvcPkga]FU-`Vn!<|'84?73z<^*5 %6pebvM<$AJT[Mo"$+r:y`JWT|+adJsOlG6]}fWG\|J_KpdFm~;[cMmN2CgGz^n7a!.t;Z\39/hV6XD3Xm?;;DpT73@|ir$1.XHT+O}L6>U{mwY':8 vjgow^;hiksyP:r~64L&eG1H+KB6KP8)s5AVihwYc_`koGhnWe^kQtA+UZ~en?,@-nveo*uhGlB^PY6C}Afz@jXaM~63(|}jGPNYQIxw}H{-*<^k'":?S`,j.uFTcS[7}?e78SG9}_HtV2}2?Yk6]DZSPW~VW<77UAEPt3AEPt3APFN APXV2APrN APrV1APrX}}ARTNASPFASPtVASPtVAUDTouB1PtUB1PtUBCTSSBufT CMNT ȕCTIDpCTNMvCTOwCTTL ȌCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA ^DATA <DATA DATA DATA 'ODATA 'O<DATA 'OZDATA 'O.DCHTDSamDySN mDye#DyeNxDyeN}DyeNȂDyeNȇDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNT-FTababFVocaQFWO_GATCFeat..a#GTyp!!LHCFGCEHCFGHCFGHCFG==InScInVtLANELAST~~QLIMS!!ȢLNTD2LsrPaMCHNMODF MODL3730NAVGNLNE`NOIS, P1AM?P1RL;P1WDCP2AMKP2BAOP2RLGPBAS.3PBAS,=PCON9PCON8PDMFPDMF,!PLOC4PLOC0)PSZE`PTYPPXLBRGNmhRMXV1RMdN RRMdV1RMdX[RPrN6RPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate @RunN""FS/N%,SCAN3SMED SMLtSMPLwSPACAp>SPAC,SPACAp>SVER4.0SVER RSVER,,Scal@Scan3TUBEA3Tmpr<UserphARphCH,8phDYbigphQLcphTRphTR