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"$!+  # /$- % #% % 9& 1($ )' !$+%"  2 !/#" !  $$  $ $"9" " ! : &%'"*&7#   '$# + !!$"% &% !"#$%& !"#$%&d,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: c87ee6c4a6574117a4aa45d0fb189bf7Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellOdontosoria_K1344_A7_trnhfKB_3730_POP7_BDTv3.mobSeqPop7z G?*qG |or  = > yIl$T ?$ B1w E # / R u E*`  U " R r o R ' O"Kjg%e7 ".451+" `8t\E/ +akh\[ u $ K P 0 v0pA5q^cB+T w x v#8'`+?.135>66 5B31e.t+'<#IT s >5T*GO_4n1n:0hi VvC y k Z @  {SqQN | y b K82>eO{fN5}aA!{{~A}/YxvU5jR?.  ,9GT`mz}fM1Z0 %4?DFDCBB@>;866540+$!&*++**+.14430-+*+++*'#  &/8AIQVZ[YTKA5+#%,020+$     !"! 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",353-$  #$   $"     (//*!                      %'$        &+,(! #&% %&#  "',//,$ "')(%   "%&$ #         !     #,11+!                    #''"     "!                                                    !'*)#     !$"   "! !#!   $%#    !%&#                         $*+(!  #)+*&  "(,*$                          "#                        "&)*(%" "##! %*,-+*)+/37:;;:999999987642/-,-02431,'#  !!"$%%#                                         &%                                                                 '08>@@>=?CHLMMMNS[dnuxyz} #7IYi}#Fe6\+AYu"!!&2CVhx0LexvP0EBN  ^ X]w$a,tK,~zwspnmlkjhikqz?n2d+77-X-`=! +JwTEiWC .  6 ]  %).:1b3J44 33<22333222100/@..0--,+B($ fyT   c K 3 I B  w d w M_}jU@, `;{uoifiq|:Sepuxy{}{tcG _8,?P^irx{}o[F/:jJy.OntDF}%}_I5#|skd]UMGDGNYfr|}xuttsqkaTH<4/-+)%! "$&''% !"  !.;DKMLHA8-!  !)-+$ %-23/(  ',,)"  &/6871)!    #''$$(+-/39=>;2&   $6EPTPE8*  )17:7/$  "1=CCA@BL[nGz#[ I"t r4><3%YAMq2a4 zndZRJC<7421/.+'#! 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(054-" 2CNOG7% ,<GKF;+   './+" %,/.'       %,38<?ABEHMTZaehiiijlmnmligffggfd`\WRMHC@=;84.("    #'&      #%#       $('#  &,,'  !$$          !   %-1/(          (/33.&                                                                         ##                                                                                          ekA҉A&AThAKB.bcpKB_3730_POP7_BDTv3.mobtermAAGGTGACGCTTTGCTATTCTTGTGATATACTCGCCGCCCCTTTGTACACAGGCTTTCCTGAGGTCTGGAACAAGATCTATTTTTAAAAGAGGATCAGTCCTTCGATTGATTAATACAAATTAATCGGATGATAGATATAACAGGAGAAAATATGTGAAATGAGGAATGGGCTTTCAATCCCTTTTCTTCCTCGATCCCCCCCTAGGAAAGACATTCCGCGTATCTGAGTAGGAATCTTCTTTTCTTAATTGATTTCGGTGTCGGATAACTTTCTACATATCTTCTTTGGATGTCCATATAGGCACCAGTTGATTTGAAAATGGACATATACCTAACTTACATTATTTTTCTTATGGTTGTTGGCAAAATCGTGGCCTTATTGCCTTCGCGGCCTAACCCTTTTTATTTGAGTGCAAAAGATGATACTAAATCTTGTTTTTTTTGATTTCACAAAAAAAGATCCATCCTCAACAGTTTTTTGCGCAGAATACTGCTCCACTTGGTTTTTTTAAGGTGACGCTTTGCTATTCTTGTGATATACTCGCCGCCCCTTTGTACACAGGCTTTCCTGAGGTCTGGAACAAGATCTATTTTTAAAAGAGGATCAGTCCTTCGATTGATTAATACAAATTAATCGGATGATAGATATAACAGGAGAAAATATGTGAAATGAGGAATGGGCTTTCAATCCCTTTTCTTCCTCGATCCCCCCCTAGGAAAGACATTCCGCGTATCTGAGTAGGAATCTTCTTTTCTTAATTGATTTCGGTGTCGGATAACTTTCTACATATCTTCTTTGGATGTCCATATAGGCACCAGTTGATTTGAAAATGGACATATACCTAACTTACATTATTTTTCTTATGGTTGTTGGCAAAATCGTGGCCTTATTGCCTTCGCGGCCTAACCCTTTTTATTTGAGTGCAAAAGATGATACTAAATCTTGTTTTTTTTGATTTCACAAAAAAAGATCCATCCTCAACAGTTTTTTGCGCAGAATACTGCTCCACTTGGTTTTTTT16L`my!/?P[jt '5=JXgr{ #;J_l| "/:EQ_kw&2>KXcp}'2@KWcn} !,7DQaly,7DQ]fr    ( 4 A K Z e p |   # 2 < G R \ j u    ' 1 > J W c q {  . 9 C O [ g s    $ / < I T a l w  +8BNYfs~'4<FUanz%/:DMXcny ",<EPZkw .@IR[gt} &2?LVcoz !*4=LWdo|+;HWakw'3@LXeoz  ,:IR^hs -9EQ_16L`my!/?P[jt '5=JXgr{ #;J_l| "/:EQ_kw&2>KXcp}'2@KWcn} !,7DQaly,7DQ]fr    ( 4 A K Z e p |   # 2 < G R \ j u    ' 1 > J W c q {  . 9 C O [ g s    $ / < I T a l w  +8BNYfs~'4<FUanz%/:DMXcny ",<EPZkw .@IR[gt} &2?LVcoz !*4=LWdo|+;HWakw'3@LXeoz  ,:IR^hs -9EQ_            /#     #&         #        (' (#'     ""                          "                    #    !         ""$"                               /#     #&         #        (' (#'     ""                          "                    #    !         ""$"                   "*,/ 8V ^V\^!!:29`5\P8&&2AB!8;bN9%>b&=@,U-a5"OFVLH V'*[_2#[4),R0b0,! W`IA1S9 F =WW/Y>P$bL"2FXB0+?T= 7L1'a AM [G?( AT?$'M*U<+)8 W>BYA'_ c#6XD'I+*"2BRbTbb92AC,EB["OQ:4WF<Uc?2!X&,36PHL /# ABN<[83 4+] ^?W 58`-[1\/G ASW RW ^K( 2XW K\!QaEMP]E20d^\=L A, X0 ;2.392G%I5`I[_2`1(d!EaYK=@OF7TFB aK32#+5S"DGB<DHB"B"-22$#8""A^=Dc'<(Q=2L*>*C':2*2:*L2U_(V_ *`3 %?Y*B2L8*N%*%2:*032*T?]:*:2*ah l {mYq ; `b f K V7t=cIzi~6.'usUn"gJ t ! L f !h?8GSUE!< *k}=  ,T'mx?,DZp8N?;Md$:b1ck1 7 ( *k8 \tTqs }@f}5Sn}`xw&AtD<401A0fZy:k)#<R/%iNCGq>y@2q,z=1vUEwp'$; D[+ -,p) }/,[$`o "A*J  # KT6a>>$nMrA{_<rJ ] bd` af0| ~g#X0 AG  ( `Y `?  OO:c:_;<BmjQ"G>"Ee<,a_ ]P/D6qr5\)(r@ KKe  t2P"<3Zq)J  `   +  ] $ .%Hdp~~:}&2KMkp<8^pufpD~SLe_B,ptPoyh#v=9 M|By I (z{{| m ^qxZEF3~VP[3N=Wh"dH ZZ{j 32JbPCd2QaHpT N(Bipmki[aR0]YtMe'yKnS  #f=V8 i_sw[/ 4l{S9$sFHk\nCGv\qD;r\WDRrWz7{`7I=<bO:8I/\<th&sio.N+VS?TXVUNc a7ngaK(KQKppi #iGS-6E\lp t)FxIGGAAGCGGCGCCGTGGGCCAAAAATTCCGGAAAAAATGGGTAGGTATATAGATTGGGAAATTACCAGTCTCACTTGATCCCCCCATTGGAGACCAACTAGGCCGATCGAGACCGGGGCCCCCCCCTAAGAAGCTTTATAGTGGTATCGTGTAGACACCTTGTTCACGAAAAAAAAGGTATGTACGCGGAGAAATTCTTGTGGGAGTACCGAGTCGAAGAGAAGAAAATAATCCCGGAGAAAGAGTGATTAATGACGTACGCCATAGCGTTAAGGATTCGGCTCGGAACCATATGTAGATCGATAGAGTTACTTAAGAGCGTGTATGGACACATCGTTCGTGTCATCAAACACCCATTCATGCCAACGGTCGCGTTATAACCCTGGAATTGATAAATTAAAGAGCCTGGAAGAGTTCCGGAGGTTGGGATCCGTCCTGCGGGAAGATAAAGGGGCCCGTTAGCTGGGAACTCTATTGGAGAGCGCGGATGACTCATCGACACCAAAAACGGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:36:25 -04:00AEPt3AEPt3APFN YAPXV2APrN |APrV1APrX}}ɅARTNASPFASPtRASPtRB1PtQB1PtQBCTSM+BufT ICMNT ECTID CTNM&CTOw9CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA 'NbDATA 'N;hDATA 'NnDATA 'NtDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȲEVNT ȾEVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~KLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS%OfScXP1AM9P1RL5P1WD=P2AMEP2BAIP2RLAPBAS'PBAS%PCON3PCON1PDMFBPDMF%PLOC-PLOC)PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%%zSCAN3SMED SMLtSMPL'SPACAo`SPAC%SPACAo`SVER4.0SVER M SVER,,ȆSatdX^Scal@Scan3TUBEA7Tmpr<UserphARphCH%phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN YAPXV2APrN |APrV1APrX}}ɅARTNASPFASPtRASPtRB1PtQB1PtQBCTSM+BufT ICMNT ECTID CTNM&CTOw9CTTL <CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA DATA DATA DATA DATA 'NbDATA 'N;hDATA 'NnDATA 'NtDCHTDSamDySN Dye#DyeN(DyeN-DyeN2DyeN7DyeWDyeW8DyeWSDyeWgEPVt!4EVNT ȲEVNT ȾEVNTEVNTFTab[FVoc[tFWO_GATCFeat..[FGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE?LAST~~KLIMS!!RLNTD2LsrPaMCHNMODF sMODL3730NAVGNLNE`NOIS%OfScXP1AM9P1RL5P1WD=P2AMEP2BAIP2RLAPBAS'PBAS%PCON3PCON1PDMFBPDMF%PLOC-PLOC)PSZE`PTYPAPXLBRGNmRMXV1RMdN RMdV1RMdX RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%%zSCAN3SMED SMLtSMPL'SPACAo`SPAC%SPACAo`SVER4.0SVER M SVER,,ȆSatdX^Scal@Scan3TUBEA7Tmpr<UserphARphCH%phDYbigphQLcphTRphTR8Bb:({.X|L=6XO?Tve!@.%J^K'p=mjzs,t5/`3T6YJ:0/.p9;4^;5s#oC&Opau_l_e5 }37Rgx|ff[`#|D5x_7&OMb'~6/lD-b)jJJTMF4Fi%P p'Icf]4'Uw\Pcc{\A_}$A>AR5Z(yHPx%bg*p/YgnP:Y'((nIcqB2t>12#\gPU=Pgt/;k$;~6>udrVbH`6@v0kc V&Ip?H^Po)Gr(K.L-[DW[Zeh&0w#j'"LAX;:bz]QyQ]bxd$XF$~8+!3@*7_6SvrsPoIXz7_lnqR9vC.Vq`A #pJE?RVuU$HTl:h~6O$@v=aiBx[FohiXN"}a8rOrm>zTJHz)RZ\oaWpnB]sVRV%.csf8=)^8HF@LeP *">BZuGZ-p@vddD\4ApZTFS$oak"T'oA_AN;)oX6YMI){mnjH[F.A[s:>ZCCd+4k,Z)lO[vHds<-F3x[R2LxC{IF}oH,k#_'>XA[.VJz$*#sejp~DsK2FtSEH$*YYo%rF(5,fX!Hiwe2{sGiSVqrj/.3~V'z(Z=kT/n@6TdO{7>kOptpuze/WR/X2EtERPV]O8oe])%,8Ea-cZ,f*F8B,}sv&'Yj}4]'xP!t p7swnqR#$|Nq_|U3tb09Z,HUcp_q5sVu7y,ra$Fl@i2+`'Mvn;l|tI)h'_1Z4=1)$#i#GF=fJX|7"WY2q>-kb+N4+,[Pom6e,F<_:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OL