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"    "     ! +,-./0123456789:;<=>?@AIJKL+,-./0123456789:;<=>?@AIJKLd,,,III^^^{{{&&&///DDDXXNaaavvv      ***333>>>RRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR8y}}{zxvutsqomljhfecbPPPGHHddd{{{  %%%--.665>>>EEEMMMTTT\\[bbbiiiooovvv|{{ !!!"""####$$$%%%&&&&'''(((())))****+++,,,,,----....////0000111112222333334444455555666667777788888899999999:::::::::::;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 5a09beee8c614e03926214731f75ceb5Seq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-16_20-47_18693130Seq18246138282_K1344_trnhfjmadera96-WellElaphoglossum_K1344_F6_trnhfKB_3730_POP7_BDTv3.mobSeqPop7zLNPTZbjptx} ,>Oas,D[s 8NdyxcM5mF |aI5$%3@MXaioty{{yupi`WK>. ~tlfa_^_`bcdddefghhfc_ZTPLIFDB>:4.)&#"""! "%).5=HUfy'Nw(251$b?<t jLQaZG]S* |m-`0 zqjc\WROLKJIHHGGFFFGJPYer+@Wo0EU^^UD,e@xsqpqstvwxxxxwvuuutsplgb[UNF?82-)&$##""! #(.6?JWfw|umf`YSNJFCA?>>=<<<>@CGJNQSVY\`dhlpsvxzzxvrokgc_[WSPMJFB<6/(" !'.6>FKMMKGB=82,&  $(**(#    #,4:<:5-$              ""     $),..,)&%&(+.0/,(#                                                                     acgmv}tlc[SMHC>93.)%!  !!"""!!   "%)-159<>@BDGJLOQRUX\ahow "3Ie ^7 C*gN wm2HuZ3 !&+./*!xrnjfb\VQMLMQW^emty}|wqh_VLD>8520/.,+++,-/26:>DIOTZ`ejmoomljkow!"! ?ur2T#j$zocXLA80*&"       "%)-0220,'      "&))($       %)*'"  !            "%(,023310../13331.+(%"! !!!!!!                                              !!                                  +:FNOKA4'       !$'+/48<ADHKNQTX[]^_^\ZWTQNJFA<72-($    !####"#%&'%5W 2IayiF$ '19>A?;3' zmd\XWX[`fmtztfZOF>72-(%#"  $+4>JWes}qe[QIC>:767899997530,)&#""$'*.268:<;:73/*%    %+/220,& #)-/.+&    #&(('%!  #'))(&$"  "$')++*(%!   !"                                !%&&%"                                                                                             !#%&(*,.148<@DHKOSX\afkqw~ )05885.#vdTG;2+%!!%*07@IR\gq{xqkea]ZXWWXY\^adhlqx+GgFu }rR8& U>O "3EXkzznaSD4$zod[RKFB?<:86558=CKS]fpy|unhc_\ZYXXXXWUROMKKMQU[ahoywof]SI@830//12479<>ABDDCB@=:72.*%!#'+059=@ABCCDDDDDDDEFGHIHGEC@=951,(%#"#%'*-27=CHLNMIC;2(  !$&&$!        !!            $(,17>FNXbmw}tkc[SKC<5/)#                   "%'*,/147:<>@AAA@@??@ABCCA>:4.("                                   >@ AAF|A33KB.bcpKB_3730_POP7_BDTv3.mobtermGAAGTGCGCTTGCGATATCACAGATATGCATATCCTCGCCTCTCTGTTATTTGTTGACTGCCGTTCGGACATTATTAAATGTTGTTAAGGTCATTTCTTGGGTTTTTTAGTTGTGATTTATAAGATTATGGTTATCGTAAAAATTAACCAAATCTTTCAGATGCTGCAAACGCTGCCCAATCTATTGGGAGATTATGAGGGGGGCGTTCATGCATACGTATTCCGCAAATCATAATAGGAAACTTTGTATTTTTAATCTATTAAATTGCAGAAGGCTATTCCAAATAGTTTTCAGAATTCAAGGTTGGAAACTTCCCATCCCCAACTTGCGATAAGCTGTTATCACTCTTTCCATTCGTTCTACCGCATCGCACGAACCTTTCTCTCATTGGACACCAAATCTCACCCTTTTCTGAAGTTGAGAGATTTGATTATTTGTTGTGCTGCATTACCAGACGGATGTTATCCGTTTTTTGAGGGAGCTTCAACAGAAGCCAAAGTCTAGGGGGAAGTTATGAGCATTACGTTACATGCATGAGGAAAAGAAGTGCGCTTGCGATATCACAGATATGCATATCCTCGCCTCTCTGTTATTTGTTGACTGCCGTTCGGACATTATTAAATGTTGTTAAGGTCATTTCTTGGGTTTTTTAGTTGTGATTTATAAGATTATGGTTATCGTAAAAATTAACCAAATCTTTCAGATGCTGCAAACGCTGCCCAATCTATTGGGAGATTATGAGGGGGGCGTTCATGCATACGTATTCCGCAAATCATAATAGGAAACTTTGTATTTTTAATCTATTAAATTGCAGAAGGCTATTCCAAATAGTTTTCAGAATTCAAGGTTGGAAACTTCCCATCCCCAACTTGCGATAAGCTGTTATCACTCTTTCCATTCGTTCTACCGCATCGCACGAACCTTTCTCTCATTGGACACCAAATCTCACCCTTTTCTGAAGTTGAGAGATTTGATTATTTGTTGTGCTGCATTACCAGACGGATGTTATCCGTTTTTTGAGGGAGCTTCAACAGAAGCCAAAGTCTAGGGGGAAGTTATGAGCATTACGTTACATGCATGAGGAAAA6Pfz  )1BN[q|#1BKZet|1<FZiy#.:FRamy(5BNZfr".:FS`l}+8BMXdr~%4@JXcoy  $ / = K W c p ~   " . ; H S _ j w    & 3 = I S ` k y    + 7 B O Z e t {  % . ; I T _ k w *6CM[fr} %1>FQ^mw #1;FR`ny  ,8EQ]hv*8@M[ft %0=IUbn{ $.7GT_ly.9ER^iw -6BL[it!+8DQ^hr)3AP^lu(6AKT\er6Pfz  )1BN[q|#1BKZet|1<FZiy#.:FRamy(5BNZfr".:FS`l}+8BMXdr~%4@JXcoy  $ / = K W c p ~   " . ; H S _ j w    & 3 = I S ` k y    + 7 B O Z e t {  % . ; I T _ k w *6CM[fr} %1>FQ^mw #1;FR`ny  ,8EQ]hv*8@M[ft %0=IUbn{ $.7GT_ly.9ER^iw -6BL[it!+8DQ^hr)3AP^lu(6AKT\er       '                   #                      '++"&      """. 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K <K .     ,l}Lpz\2ATVplR@"EXor 3f~lpU$W|[ukuiyPj y\ zaj5q20pAHYhf2}SJ"v37a?8~{fGc,Xda0|wLw8NFX QY*>N$>vlBvO7?)18 2`!LD wp;b c4|6h~L- }3hpMp&OnWRp25);!E,p\}i Xd|1J.qp^Ckr?NJN^6 (tN\' N N*\E @P [&a;$`H"*kRAbAV@n2+;tdVRK8KX'1K.0`xKN :?:_D,jm=% OzO?_,y41zK&7M]vX,0+"A)0?Leu>~u& *\^Ph *">\9y'q9s JjcqXy2Vlsn6DlqV)RZiPW7S o?+: .iHJ4Z*"x<>M}Gx{DsZ`X.d)w &+"Z<~ HBTt97Eft@qhh7' rt9 Y@&5/*1~Nf/SUQ`reX wlaIz:Prih+(-fv>bt&!JoAGGAGCGCGCGAGAGCCCAGATTCCTGTGTACAAAAACGACTAAGTAGTAAATGAACACTATCGAAATCATCGTGATCTACCCCCATGAAAAGCCAGAAATTGCCACCGAGACAAGCACTACCCCCATATCAAGAATGGCGGCAATGTAGTTAACAAGCTGAAGGTACTCAATGCATACTCTGGGAAAAGATAGGGCGACCTTATTGAGCGATGCCAAATCCGAAACCTATTAGGGTAAGGCTGCCTGCAACCATTCTGTGATGGAAAGCAGGTTTCCCATTTTGGTTGGGGGGATTACTTGTAAACTCTCAACATACCAAAGCTACCTTTTATGAGGTGGGGACTGTAACTACCGTCCAAATGATTTATATTGCGCTTGCCTCAGTCAAAAGACGGCGGGGCTTTAACCGAGGTGCCGATCTGACAGGTTAATCCATGATATGCCGCAATGGTACGACGACGCTCTTTAAAGGGAGAAAGAGGGGCGGCCCCCGGCCGTCTGGATAAAGGAAATGTGTGGGGTACCCCACCAGGCACCAGGCTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-16 22:36:04 -04:00AEPt3AEPt3APFN APXV2APrN ɤAPrV1APrX}}ɭARTNASPFASPtiASPtiB1PthB1PthBCTSMBufT qCMNT mCTIDHCTNMNCTOwaCTTL dCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA 6DATA DATA DATA DATA &MDATA &M; DATA &MDATA &M DCHTDSamDySN EDye#DyeNPDyeNUDyeNZDyeN_DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!$HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~L'LIMS!!zLNTD2LsrPaMCHNMODF ɛMODL3730NAVGNLNE`NOIS#OfSclP1AM @9P1RL @4P1WD @=GP2AM @EP2BA  JP2RL @APBAS  %PBAS  #PCON  2PCON  0PDMFlPDMF#PLOC @,GPLOC @(PSZE`PTYPiPXLBRGNm@RMXV1RMdN *RMdV1RMdX3RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%#SCAN3SMED SMLtSMPLOSPACApSPAC#SPACApSVER4.0SVER MSVER,,ȮSatdl^Scal@Scan3TUBEF6Tmpr<UserphARphCH#phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN ɤAPrV1APrX}}ɭARTNASPFASPtiASPtiB1PthB1PthBCTSMBufT qCMNT mCTIDHCTNMNCTOwaCTTL dCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA 6DATA DATA DATA DATA &MDATA &M; DATA &MDATA &M DCHTDSamDySN EDye#DyeNPDyeNUDyeNZDyeN_DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab\ FVoc[FWO_GATCFeat//[GTyp!!$HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~L'LIMS!!zLNTD2LsrPaMCHNMODF ɛMODL3730NAVGNLNE`NOIS#OfSclP1AM @9P1RL @4P1WD @=GP2AM @EP2BA  JP2RL @APBAS  %PBAS  #PCON  2PCON  0PDMFlPDMF#PLOC @,GPLOC @(PSZE`PTYPiPXLBRGNm@RMXV1RMdN *RMdV1RMdX3RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%#SCAN3SMED SMLtSMPLOSPACApSPAC#SPACApSVER4.0SVER MSVER,,ȮSatdl^Scal@Scan3TUBEF6Tmpr<UserphARphCH#phDYbigphQLcphTRphTR4{,|Qv%F}K7T]Zj7y!R}|MCZ9zvr3H}T"mqD-WeDmq$a~8;R/;8-b#DI#k>4L\\W'KFxz2jA&owbor~Mu^yu6v;(vJ4;iD4ZI,nG9T+8@/Sco6m~2HBIma#'LyHHR&UTlsQml{W7;rRU)>`V2]<|'kp?_sDk`457^^s?92>@iG-@,P> u)v/|vu~CM#Y\Vd*U5aO/CsINi@C,{s2fo;LY&S2Bjr<|Ate1}1"/"@-bWF4oFo;X.: wT_fuwQEnfD~VJ(/on}sF{AR8=(V-OGC#!^MF]kT\?YqvFYli&v6=+ew.hFfm&W-O]!zBwfmk=;A{%iTK(>V&U)>`V|I+!K|-o*.AQ>UW!\`C}B-8'i2{2[P/ j\V'i~8m#ulc*{<=pjHOo&eG1Vc#TE"J%mydmr+8/Yb~;H26A{%?_CjLhhSc>7:I1DD[ho* xv'o>s1HT/(J8dR9JK[Yle9Su3*-CdM]4e):EGa9?QLwUF3TP.&"D|;f[5kr+E{'hIU%Ja9)R9J4\E|Gf]9$~#*Gj6PA-]lbZ]"Po1Cd}F<_`VWYH|O[nY\Kwoh^*p%!++(aQQ*G]mysG(B]mzQLAEPt3AEPt3APFN APXV2APrN ɤAPrV1APrX}}ɭARTNASPFASPtiASPtiAUDTkB1PthB1PthBCTSMBufT qCMNT mCTIDHCTNMNCTOwaCTTL dCpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA 6DATA DATA DATA DATA &MDATA &M; DATA &MDATA &M DCHTDSamDySN EDye#DyeNPDyeNUDyeNZDyeN_DyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTab\ FVoc[FWO_GATCFeat//[GTyp!!$HCFGCEHCFGHCFGHCFG==InScInVtLANE&LAST~~L'LIMS!!zLNTD2LsrPaMCHNMODF ɛMODL3730NAVGNLNE`NOIS#OfSclP1AM @9P1RL @4P1WD @=GP2AM @EP2BA  JP2RL @APBAS  %PBAS  #PCON  2PCON  0PDMFlPDMF#PLOC @,GPLOC @(PSZE`PTYPiPXLBRGNm@RMXV1RMdN *RMdV1RMdX3RPrNRPrV1RUND RUND RUND RUND RUNT 0RUNT #RUNT  RUNT  &Rate RunN""S/N%#SCAN3SMED SMLtSMPLOSPACApSPAC#SPACApSVER4.0SVER MSVER,,ȮSatdl^Scal@Scan3TUBEF6Tmpr<UserphARphCH#phDYbigphQLcphTRphTR