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'    -   SP\                        d,,,III^^^{{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR9}{ywusrQQQHHHfff}}}      )))111999BBAIIIQQQXXW___fefmmmssszzy~~~<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 6ba7192124fe476f94558233bf2b769fSeq_4000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsetruefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq_4000 Seq_40001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time4000Seq50Pop7Z_4000Run_3730NHB_2017-03-17_00-07_18713130Seq18248138283_K1344_psbafjmadera96-WellAmauropelta_K1344_B5_psba3fKB_3730_POP7_BDTv3.mobSeqPop7z1G_|5o4} zJQ2 x  c  y?W6jgARX@ d  i " n >  { N n > [#T!rW@*Sf'q?tnqzqS5hG+ '8Mh(Lt M_3m]d J B  0 B 9  v  ~H'd3h6)~5s [ 9 F  ` ; i)~z{ t ]VcMu_RI<*D6; ,Nt!CY^R7wErXH@?BGMU`p"JknR1 jL5#  &.6<>=93,%       #/Dc5v$$oTKTp02r$Z2 !,6@FHD:-"/:BFE@8-!  $8M`owvm^I3 !&*,-,(#'>`oR:&  ?qem 8I#Q]Fe _KB=92*!    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L Y e q   '3>IT`mx  , . 5..  %'!"/* *  &"*3&''2'3* '32333,/33&8'# 2 # *3',,#*3 3 2,*# '& &" 3** ! &&.>//>>>>>>>.8>>>>>>2&**>>>>>>8>8.88//88/)6+>88>>>>>>8666>.>+>4/44+"       ##"    . 5..  %'!"/* *  &"*3&''2'3* '32333,/33&8'# 2 # *3',,#*3 3 2,*# '& &" 3** ! &&.>//>>>>>>>.8>>>>>>2&**>>>>>>8>8.88//88/)6+>88>>>>>>8666>.>+>4/44+"       ##"   P13#A^N9?^M42-L/7 N7(UM(/ X;#U,K6@S#M %"@P<94F66Z)1/-X51T]Z,N##RW \0>+^+7L\dQLHc''VSG%0UAP^LH^2I #[ KA_88V=$0ZRMI *2  c GV]^D*Y"/;='=X1R\ )Y"GDVXU^5+PQ$/P"ZEB6 7+P E38%%J\.\'9c^?B[d 6E36>4+_H 6)![1X]?B>)_$ %4W-OE&M(D c[aT7XK,_Uw o p Z] P  !ty)Tf%Le+zM  |[g1l*K jX3iZgT nJY vxvIj|M~y&39:N_V0pVhEil~h4iw@ 4qV:yS 53s?HZGMrKWv$;9vp6[gH?-TTu  >%t7jEICqLW(>pOWp-pT?z Vun[d}?;xWC:-:J0Ts^jV}eKcWNZco^VNMLY;B(yC8fX-1@=.?jp!&"00=7G6%#")*+ 8\8T%:.&("A))-1(   0<A3%#  !3 4 &7""2& +)%     1;(" '5M& %H#!/4*8"2$+#A!$/)4Gt0:32+!(    ##'(@&.  )###)K )S"xp&8 DpTPS,-pA`UbedO&:O#I*tzp,z8^h!u8'EUv288p,Uhx8pBd_jug8N>]85Hr3c^888ddW@R{Rm8p88$^| w + =F Se ^56b3N/@9cc4-n=fgxyhm I)pGay?X'|>Pw1#01)R:817Z[6T  .fV`<C%XyU+5(@=]/xi /?o!?[aG/.:+085EBD%@!) .23[RcD)$P;d6MQw[FTUn9M_Q4*;(aQWV_Vv^=JIZT)QpW1m7vyQ<KOd`^i >o~ZF?GjqNGGTTTTTCCCCGTGGGCGAGGACCTCTTACTATACTTTATTATTGTCTTCTGCCTAATATATATGGCCTCCCTACAACGTGAAACACTAATGAAGAATTAGAAAAACACCCATTCTATTAGCTGCAGGATGTGCCGCTGTAACCCAATGATACCTGCAAGTGGGATTGGGACTCCCCCATGCGCGATCCAAAAAGTACGCTTGGGGCGGAACCTGCTCTTCCGACTTGGGGTGGTTGGATGAGGAACCTGCTAATAAGCCAAGGTGGCAAAATGCTAGCAATCTACTCCCTCAACCTTATGGGTGGTAGACATTGACTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82017-03-17 01:37:56 -04:00AEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTS;BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'OpDATA 'Op4!DATA 'OpDATA 'OpDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFWO_GATCGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~:YLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS"yP1AM?~/"P1RL?~,P1WD?~1P2AM?~6P2BA??9P2RL?~4PBAS??#PBAS??"PCON??+ePCON??*&PDMFPDMF"PLOC?~'PLOC?~%*PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%"qSCAN3SMED SMLtSMPLuSPACMSPAC"SPACMSVER4.0SVER ;SVER,,nScal@Scan3TUBEB5Tmpr<UserphARphCH"phDYbigphQLcphTRphTR&This is the confidence range *ABI_Limits..FVocAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtB1PtB1PtBCTS;BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'OpDATA 'Op4!DATA 'OpDATA 'OpDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabJ=FVocJ,FWO_GATCFeat//IGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~:YLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS"yP1AM?~/"P1RL?~,P1WD?~1P2AM?~6P2BA??9P2RL?~4PBAS??#PBAS??"PCON??+ePCON??*&PDMFPDMF"PLOC?~'PLOC?~%*PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%"qSCAN3SMED SMLtSMPLuSPACMSPAC"SPACMSVER4.0SVER ;SVER,,nScal@Scan3TUBEB5Tmpr<UserphARphCH"phDYbigphQLcphTRphTRqCfkNR(Bj'~;Hk@*w&RA#] lM6[/:){hV(wX>?7u%#F7Np1mj;RJJ4R,9X PMF9zH'zAuR)x|ec{PG6x|W"OKOfx&i1PBEe>2flV:2/dmm YJX?/WZ#}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMGg_1A([N((nJy:]@|Q$OEH:6\"B-Y@iGtX,P> u)v=i57~+lF{YTl_{1#(7GDT`h3/[zr|R5/*7ag%gcmeaooUzbaI232)O~P@abHBa+b8EY59D4*Ry,xPMbx5I89uV3Qs>xvU+}<;,v7RP 5Go-D7I 9xxsWD@4ir$GE1Xz.:Axe<>/D?*Uq$[-g]A8c[28N.-rMVExi;3M'BL nsu9-]H6X+9muy%lFU.F4/oawfT;GAzr|ik[}7-jAS_7WX7a^]*RiTK(>Vq&s1Hw,RI[xsU3T%ycX}O!DZNu@vulc*{d&]?#:{CsU|}&_[3A4zW2`&TA]BGmb,tJLVudrV=y`6@vulc*{7=hI8J)T(k"T'o0_s2#O|!Y<,h`+m&wE6Gz zF/"@>oW~!zFHyErN3W4KD@w JU6PX `HO5QfOl>o XSudrH>PWYQIxwcL*L@rIwYWgUTjLj.u%xfmq;MmN2CgGz^nLoT.i:"\oE):|j!]3B68l|ap'Ic3XkSnwkI6 +FwMzbU`AtGbI.J:*yEwiOOd&79?Ou+:n%ra$NwBx6Ks;6R&)Y?Z8Z~9}_{J^z(aT20p/(ZA$den?,}& b!a7QAQ>UbeP *"Y3hk?^NHE,v*(]BcYAFc=gMAEPt3AEPt3APFN APXV2APrN dAPrV1APrX}}mARTNASPFASPtASPtAUDTXPB1PtB1PtBCTS;BufT"D1CMNT -CTIDCTNMCTOw!CTTL $CpEPDATA3gDATA3ghDATA3gЪDATA3g8DATA"D^DATA"DDATA"DDATA"D*DATA 'OpDATA 'Op4!DATA 'OpDATA 'OpDCHTDSamDySN Dye#DyeNDyeNDyeNDyeNDyeWDyeW8DyeWSDyeWgEPVt!4EVNT EVNT EVNTEVNTFTabJ=FVocJ,FWO_GATCFeat//IGTyp!!HCFGCEHCFGHCFGHCFG==InScInVtLANE-LAST~~:YLIMS!!:LNTD2LsrPaMCHNMODF [MODL3730NAVGNLNE`NOIS"yP1AM?~/"P1RL?~,P1WD?~1P2AM?~6P2BA??9P2RL?~4PBAS??#PBAS??"PCON??+ePCON??*&PDMFPDMF"PLOC?~'PLOC?~%*PSZE`PTYP)PXLBRGNmRMXV1RMdN RMdV1RMdXRPrNRPrV1RUND RUND RUND RUND RUNT RUNT % RUNT  !RUNT  '6Rate RunN""S/N%"qSCAN3SMED SMLtSMPLuSPACMSPAC"SPACMSVER4.0SVER ;SVER,,nScal@Scan3TUBEB5Tmpr<UserphARphCH"phDYbigphQLcphTRphTR