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***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR(Q`eggggggfeccba`_]\ZXXVTSRPNLKIHFDCLLLEEU`auww   #"'**23399:AAAHHHOOOUUU\\\bbbhhhnnnttszyy~~}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8bbc2367550744cda584e4b70b51ad64Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-12-09_07-01_20633130Seq15467131875_sgoyette_131875_1Fmfagan96-WellK1346_E4_ESRBCL1FKB_3730_POP7_BDTv3.mobSeqPop7zbj{=m)l;%|/FxB H $ d  # #   f . } 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}Ce/]=eme-OY_ 8:#1Cx"rg>WIg_R,J.HTI)d4R }Q5IsdY-+dc}h "_p3K6)GGAGGAGAGTGAACACGTATACGGCATCCGTAAAGTGGCTATATACTGAGACCTCGAAGGACGATTGTGCATCGGTTACACCTGGGGCACCTGCTTCGATATGGCTATATCGTTTTCTTTTACGTTTTCCCATTTATTTTTATAAACAAGCGCTACTTGATGATTTTGATATATCTCCACCTCTGTACATATTTCATAACACAACATGCCAGTTTGCCGCGACTTTTTACACCGTCTCACCCGGGGTGGAGGAAGATGTCGTGGTTGGGCCCCCGAAGCAGCTTCCCAGGCCGTATCTCTCCGCCCATCCTGCAGTGCCACCCCTGTGCACGTCATCATCAGACCCGATGACCTCTTACCCGAATATAGACGCTCTCGGCGAGTTGGACAACCTTTTACTTCAGGCGCCTACTACGCCACGGTAGTCACAGGGTCACACTGTAACTTTGGTGGGAAAGATTTAGTCCACCTATGTTCACCTTATAATGGTAATATGCACCCTCCCAACTTTAGTCTCTGTGTCCGACCGGTAACACTGTAGCGGTCTACAAGGGCGTCCTCGTTGTACACGTTAACTCACCCGATAAGAAGCTTATGAGTCCTGACCGTCCCTTACGTGGTAGAGATATTGCCCTTCTAAGTGTCGCAGGCTCTCCGCTCTTATTACACGGTGCCACACCAAGTATCGAACGTAATTCATCCACGATTCTGCCTGCACGTGACAGAGTAATATGGCGGAAGCGCTAGACCTCGCTAAAGATGTTTGCGCATCTAGTTGACACGCGTCTCCATCTCCACAAGAATGCTCCACGCTTACTAGATCAGATCTCTATATCGCAGGATAGAAAGACTGCCTCCCCATACTCAGCGCGCTACAGCGGTGTTATGGGACGCCGCGTTCTCGCTGTACGCAACCTCGTGTAGGAGACCGCGTGATTCCTGTCTGCCGAGATATTATACTTGCTGCACACGTGTGATACACCAAGAGAAAGACAGGAATATCTGTTGCTCTGATATATCTGCGACATACCATAGGAGACGGCTGGCTAAATACTCACATAGTGATCTCCGCTTGATTCACCAGCCTTGCAATACTACTCTACGCGTCCTACAGATATAGTCTTATATCGCAAGACTAAGTCCGGNTTATCCAATGGGAGAABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-12-09 08:48:55 -05:00AEPt?AEPt?APFN Y!APXV2APrN 5<APrV5APrX~~5EARTNASPFASPt(ASPt(B1Pt'B1Pt'BCTSBufT O&CMNT 4CTIDNCTNMNCTOwOCTTL 4CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA  DATA *mDATA ?VY*DATA ?V؀DATA ?VWDATA ?V,DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN4DyeN4DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4kEVNT 4wEVNT4EVNT4FWO_GATCGTyp!!4HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~LIMS!!5 LNTD2LsrPaMCHNMODF5,MODL3730NAVGNLNE`NOISVOfSc0 P1AM !P1RL { P1WD 5P2AM ]P2BAqP2RL IPBAS[GPBASVPCONvPCONqPDMFY PDMFVPLOC hPLOC _PSZE`PTYPOPXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND  RUND  RUND  RUND  RUNT RUNT 0RUNT RUNT /Rate 4RunN**NS/N%VSCAN@SMED SMLtSMPLXSPACAdSPACVSPACAdSVER4.0SVER ySVER,,4?Satd0Scal@Scan@TUBEE4Tmpr<UserphARphCHVphDYbigphQLcphTRphTR1This is the confidence range *ABI_Limits..FVocAEPt?AEPt?APFN Y!APXV2APrN 5<APrV5APrX~~5EARTNASPFASPt(ASPt(B1Pt'B1Pt'BCTSBufT O&CMNT 4CTIDNCTNMNCTOwOCTTL 4CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA  DATA *mDATA ?VY*DATA ?V؀DATA ?VWDATA ?V,DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN4DyeN4DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4kEVNT 4wEVNT4EVNT4FTabFVocFWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~LIMS!!5 LNTD2LsrPaMCHNMODF5,MODL3730NAVGNLNE`NOISVOfSc0 P1AM !P1RL { P1WD 5P2AM ]P2BAqP2RL IPBAS[GPBASVPCONvPCONqPDMFY PDMFVPLOC hPLOC _PSZE`PTYPOPXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND  RUND  RUND  RUND  RUNT RUNT 0RUNT RUNT /Rate 4RunN**NS/N%VSCAN@SMED SMLtSMPLXSPACAdSPACVSPACAdSVER4.0SVER ySVER,,4?Satd0Scal@Scan@TUBEE4Tmpr<UserphARphCHVphDYbigphQLcphTRphTRr/)ecAA{!9Y2]C0nD2,3D?x%ZH|x+1[n\PF7^%4G}UlL`QF_4}V3Er]mz]~C*/meqy{g_hD6E{wsUfGX.!6^q;,`h?X??-|D>){6ft[XS<< x3]aC^D~i#aYP+p?H(+:MkO@JC,E`fW7.5k|_%Z]R'OEte1}1"/"g7bxd$XF${WhcS]U$3g;Z6ObePWz"$b[q5FaT%ycX}sP:gtqbg(*2K]?ZO~|Ue_v#a}_#S!KnMfgmwgE9J3Q{Y>~mB64<UW!\`C}B-mq5FaT9Vbr$rv/gg61CNdW)j-[YD8J)T(k"T'oA_AN;)oX6YMI)0g'^+oL.SVX*os7}Z&['Ko;X^`>EOCy KQI5m6``^,Ffn@axsA MG }OPmE$ww*{we* _ZZ^"4/GKTrQ3oS"Y|8by`AVih{3r< wvr]LaiY2]NDzU4obir8rP p'Ic|4`A(^:go8m+adJs _ZZ^USn&vrOU[.(3`C/fshkr+E`_W>WM@A<}1X*#PE|Gf 9 ,#w#ulzKq2OZ;DA>iv<("Hf.QbU`AtG>udrV|gwCiOfF{\)lOYW|_nw|]jTj.uFPqWgZ*me2&dArXx6m}3,~~+N@c'v+gK >o>s1HT+O}L6>U{mwY1XV7:>JW*z(KC,Zy`[qDr_WG\|xT;#6RlSR5[h-er5!_uJUo6X?/WZ#3|5Dfqcd o;X.>S]T'_C:q|4G,fcp,@B_8Es{jGyZ.E/zC&/VnZAEPt?AEPt?APFN Y!APXV2APrN 5<APrV5APrX~~5EARTNASPFASPt(ASPt(AUDTrr̲B1Pt'B1Pt'BCTSBufT O&CMNT 4CTIDNCTNMNCTOwOCTTL 4CpEPDATA@DATA@DATA@DATA@DATA  DATA DATA  DATA *mDATA ?VY*DATA ?V؀DATA ?VWDATA ?V,DCHTDSamDySN 4Dye#DyeN4DyeN4DyeN4DyeN4DyeWDyeW8DyeWSDyeWgEPVt!4EVNT 4kEVNT 4wEVNT4EVNT4FTabFVocFWO_GATCFeat//GTyp!!4HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~LIMS!!5 LNTD2LsrPaMCHNMODF5,MODL3730NAVGNLNE`NOISVOfSc0 P1AM !P1RL { P1WD 5P2AM ]P2BAqP2RL IPBAS[GPBASVPCONvPCONqPDMFY PDMFVPLOC hPLOC _PSZE`PTYPOPXLBRGNmNRMXV1RMdNFRMdV1RMdXFRPrNNRPrV1RUND  RUND  RUND  RUND  RUNT RUNT 0RUNT RUNT /Rate 4RunN**NS/N%VSCAN@SMED SMLtSMPLXSPACAdSPACVSPACAdSVER4.0SVER ySVER,,4?Satd0Scal@Scan@TUBEE4Tmpr<UserphARphCHVphDYbigphQLcphTRphTR