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"         # 3  )              $                                                                  +2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 506f2ef423ea4d24923cdc14277f1434Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_H9_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z %0;DJMPTZdp~ #.=V|#['Q L)_Cix[8^7]#nWF:1*#wX:qDn[H7&|ofb`_]YSLFCCHNV^ejmnlgaYRLGB>93.(#   !   %(**'#  $**&$)*)&#""#$%%$%&'((&$ $).258985.%  #&()+-/25:AIRZ^^ZUQRYet{vrpoory Wp= dL=9@RoFL'`+J'$+3:?BB@<977:=@BBA?==?BEHJIFA>?I^Hwt;cF3' #5Vg./nO&  *5@HMMJD>8310037<AEHIGB=98:@IQWYWQJC=856@W}ED^cQ(qG9Hnr.i; &<Wv4B@/sN/   &J\cG! 3V,Xt{mJ{[KHNW^`]XRLE=2&  (W=jy> "(++)#          ".42*  )2:CJMLG=1&!(/47640.--/12221/+&$<d?`nhP+nK2""',035568=FThoQ=1*'$! !$'(%   'LCr]$a4     (-0.)!  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'[: px2I *PdH&p7|HDgz Opfeppdo~>~8JF=d7Y_Fp888h;PLO5p  ,8|w#18 d:8G80 pTppt;p8D8ikVpppppfLpp4,,{C8pp pp^,K8,pp8pp,ppZ8pp88pp,pp&pp5KVz=tr;HkVSfDu w07*D.X7>-O.G"H2[&_I`dVZf3aBW,rk44uu^C5<8]LN\x?Tf TRa1K8]SqMXzhwA2)c_S[~A_B2e@DA}lC9STadXS^jo Zw. &AL6>+|c e"bQ^xt8QxiCpfXpCJ z/PG'Hg9q6B*iOuO/yD=cxD! 6vxBv}{)HQuTS)ed?A1puSPzh <D>tY!@ITESi2yX'bW,z=;Otosw0iWuw;y/(<jK `b|9(4J@wWR<5J3VSk jHf@FW:)gBdD%kNL|UNS/._<[f&r(&=jgf^Y%0 iVK}IZbXP>'6 G~JdjRp2vrL'P1j`J9SY N R: szA?*Qnf+Pd~!#B9FO4>cw. !zo0C*CAAAAAGAGATATCCACCCACCACCTCGATAGAGTATACCCTACGACGCTGACATAAAAAGTCACCGGAACAACGTTAGAGAGAACCGACCCATGTTTGGCCCGGGTTACCCCACCAGGGGGGGACCCAAGTGTGGGGACGGACTCCCCCTAAACCATGAAGATTAGGGGGCGCCTCTAAGAGACGTTTAGAATACCGGGAGCTACACGGAAGACCCCCTGAACCGAGCGCCGTGGATGCCTTAAGATCGAAAACGCCATACCATCGCGGAAGATGATCAGGCGGAAGACACCTCGACAAAAGCGGCGTTAAGCTCTCTGTCCACCTCCCGTTGCGGGTTCATGGCCGCGGGGGAAACTTCATTCGCCCCGAACTTACGTAAAGTCCTATCTACCCCCACAAGTAGACTAACGTGTATTCTGTCCTCTCAAGCCATACTTGAGTTAGAGGTATGAACTTAAAGAACTTCCACCTTCTCGAAACGGAAGTCAAACCAAAGCCAATACCTCGGAAACGGTCACCGTCTAAGTGTCGACGGCGGAAACAAGCTTGGCGCTGGTCTTACCCAGTACCTGGAGAGAGACCAAAAACTCACACATCAGCCTCGGTTCGCCCTTTAAGGATTGGAATCAATACGGCGCCAATACCCGTTCGAAACAAATACCTGAGCTGTTGCGGGGCGGTTCGAGACGCACTCACCCCCATCGTAAAGACGCCGAGAATATGACCGTATACTTATCTATATAGTTTGTTACTCAATACCATGAAGAACATCACATAACACCGCTTCTAACCATGTGGCTCAAGAGCAAACAGCCCAATACCGTCTGTATGCCATACTCTGGGGAGGACTTGCGGATCCCCGTCTCCTTATATAGGGCGTCTCAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:22:02 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt7ASPt7B1Pt6B1Pt6BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?\TDATA ?\DATA ?\SJDATA ?\ҦDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANEALAST~~8LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AMytP1RLynP1WDy{P2AMyP2BAyyP2RLyPBASyyUPBASyyR=PCONyyjPCONyygPDMFTrPDMFR!PLOCy`!PLOCyY/PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%RSCAN@SMED SMLtSMPLT^SPACAhSPACRSPACAhSVER4.0SVER SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCHR8phDYbigphQLcphTRphTR This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt7ASPt7B1Pt6B1Pt6BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?\TDATA ?\DATA ?\SJDATA ?\ҦDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVoc FWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANEALAST~~8LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AMytP1RLynP1WDy{P2AMyP2BAyyP2RLyPBASyyUPBASyyR=PCONyyjPCONyygPDMFTrPDMFR!PLOCy`!PLOCyY/PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%RSCAN@SMED SMLtSMPLT^SPACAhSPACRSPACAhSVER4.0SVER SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCHR8phDYbigphQLcphTRphTRTFo@L15@FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(6(1'q$xhp k+3h)9OjnlfP_UqN^Fu*tayr=u4|8e7s.FSKpuze/M#yKA[,5SZ=;WXI p*][oTsr*G6`VA=M;_}$A>e6bZ( %P^gWTRI1]f)yA@r9G4/CeG1V+&hyccSIoM/V/5`agvb_@U])%,8EWtixy$|FRrtlqaxkabCDkSnk-~4o]R03jaUFGsbrudrV3]NE^O{3fJ:h94a7{\r0Kb!f}tI~eeZDmEAo!:Z`+a6:!.tkSY4E2rfJX|PzMWhceS-bb3SvrsPo"`dbp~lV}sT!lZbCd]E>]H\#D\mvH%lFU~ZYWm;2FV]#[,e/8I[X1EDE?]Rc{_@=K{_"#kt72WeLWLVlcG`a[X?YCC/3[`kI ?"U27`N`3[)|$X[@5+~.m"J$xi&MJk7*mE?^zcHSF{,ComMOx8yT,I)_|_n?_;?oW~!^F7N? >S[jWcgEQm*Y6i47.r~+:mzv&1`Zkel{F&+?cfoQe=N^tpuzemMOMh7&NxOrz_l4~h@<}9W#()rR.X}e6bZ( ]'nya0#CFlc*{hAC?-4$&"Sf^wKB]A\9I*$6elkgahH;M[=g;^{AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt7ASPt7AUDTvv/B1Pt6B1Pt6BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?\TDATA ?\DATA ?\SJDATA ?\ҦDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVoc FWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANEALAST~~8LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISR P1AMytP1RLynP1WDy{P2AMyP2BAyyP2RLyPBASyyUPBASyyR=PCONyyjPCONyygPDMFTrPDMFR!PLOCy`!PLOCyY/PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%RSCAN@SMED SMLtSMPLT^SPACAhSPACRSPACAhSVER4.0SVER SVER,,/Scal@Scan@TUBEH9Tmpr<UserphARphCHR8phDYbigphQLcphTRphTR