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"                                                                               !      $1                                           %      *                                        d(<,,,III^^^{{&&&999DDDXXXaaavvv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR=BEFFFGFEEDDCBAA@?>>=;;8864420/.,+*JJCCC^^^sss   ###+++232:99@@@HGGNMRTTYZZaa`ffflllrrrwww}||+++++++,,,,,----....////0000111112222333344445555566667777788888999999:::::::::::;;;;;;;;;;;;;;;;;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3a2efd0a350e449e8f609552594b80e2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_09-35_18873130Seq15259131435_sgoyette_131435_1Fmfagan96-WellK1344_H5_ESRBCL1FKB_3730_POP7_BDTv3.mobSeqPop7z-Xw /JZbjxIq5yQ."  n 3 i X8~U&c,?B,4r) { P v 4  * 9 8 )   ! 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I S _ j w    + 5 A N Z e q | '3>JValy)8CN\hp|#.:GVaox!-7FR^iv +6COZgu%1<HVal{ -9FPZfu (5@MYbmy /8HS\jt",9DMZbox &2:HQ[ JP[aq| +<Ndq{*8EP[gs} .:DP]jt-8DO\ht$1>IUao{ $0<GS]iw  -7EO^hv'3BNWdp{    & 3 A K V d q y   % 2 < F S ] l w    + 6 C P [ f s ~    ( 2 ? I S _ j w    + 5 A N Z e q | '3>JValy)8CN\hp|#.:GVaox!-7FR^iv +6COZgu%1<HVal{ -9FPZfu (5@MYbmy /8HS\jt",9DMZbox &2:HQ[                !             !                        ""                                       "                              !             !                        ""                                       "              D)*'/E0L+A+'Q3L][b"/J. 1Q%_.2LP6B\<N Nb`$S2J@5R$a^GI-;,L$>O cV;QFE.)7#QD .3%F< Z?OW0bL))6ZG_@Q1 `C %KM=]*PN-%\RF'-d<J@ *7[6KKX2 bE>_Pc  :171$3I 2@[OG"B<3;L3 .B!a$ #WW:'=,>0UKF4K*37;/N: 68F'MWSR ,%!$K19c?C6E$U4ZEB9aG$5M<-^ 3' ,WPG]L8PE9Vd,C]5R@'F,Q -K;1 !-H2PY6,@<*]B!)$N&("3; 2+JZP"S@QY>ROH.L._ )*b )EN8X;d&H LXM1'E-%:(%J:;U.1EG1O7Y7/-:3=]^SH!_% F CTy& 9 "   &Hj:Ayyw(na Npa\XO(748mk[2'OhmttR{"&#.PT8R/Rpew>oHW?b1RZTxdZ4t&TD:dO*v0unWvY$-3r`yj ibJ; P<I7@9Ma  ~=@5:lu/q_#YM5"X)- Qg>Xv6|1NTSj'+H[w<VV GVm!4vI8grCuw\}Zdht$s<[}c[4+>D{+4Y @kTR+%6a2JUc-}$256' $pqO W6 e>o\]n8|&:[9NJ3pan_atjhCKgRX<DQxDxJ= .i:4D$<e0$W-;V HF@UO+ 849J#+kv\w`Kj(_Gf`)nY_V>`^@2=  M8&]*01'DhF >1L ?g CrL O:$a-"cr   3C ]0%~_4zp@e(D m 9% 6{$C:@jO]+/] #*>PXX9h#D4>!21(%$1? 4@m+9V+ +\Nk(>$7A*<mU $9I'".~,l%8Sr%$"3`)90K>;)e &Vlf"$L7 *$]?S8/,pp\TTz*I b>K%Cr |>mC]&,)6^p"_ssEk ?v=93+pK<p7TCY>iD*Hkv#\~Xog5\N9kfpPbm^aX|N*r|7mp p;p}U, ,EsB1N;(3h,n?m^b%yR7 4V'JQzl%j/\*.p#4vdDn1Xq,% *L;e92|M:,dJA *9'@CMcED ~p<'p,<nTf$h$\{sS) ]=W1,.-dqd} - o'- '}h2 -QCpi9T9 v~~N ._F@;\nhx_zNF&>KDQ5XrsFLq}[W<O7r$r1|&1 n~[PtcO jo/(G]^\:bY>HPX?"f>mP|Rx- 2V cxo+=]P p[\6\C;Pwc{R"Qd)P)Umv4/Q3\qM;_;1O'v<9B6@JmsO!QMg lYNEIaN{%^nhFM3)8 *8 ZACaTGTTGGAAGTGATAGTAGACCTAAATTTACACTGTTAAATTCACTCACGAATTCACACGCTAGGTACTCCCCCCCCCGGACGCGCCCGACTAGACCAAAACACCCCAATTATCGTCGACAGGACCTGGACTTGCCGTACTCGCCAATCCCTTAACGCAACATCAGCACATAGGTTTGCCGCTTGTTTAACTTATCTTGGATGTTAAAGGGGCCCAAAGAAAAGAATAGAACCCGTTTCGTGTAAGAGAACCCGAATTATGGGGTGTAGACCTTACCCCTGGGAACTATCAAAAAGGAGTCTTTTCTACATTTGCCCCCCCTCATGGAGTTTGAGGTTTGGGGATTTATTCTTTTCGCCGCCCAGGCTTTGAAAGCCATAGAATACCCTGGGGTCATAATATATTTTTTGAGGATTCCCTTCGTACTAGTCTAGCATTAATGGGTAACAAGAATATCTAAATCCCGCTTCCCCGTGTGTTGGAAACAACTCCAGACTAGGGGGTTCTCTAACBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 11:34:12 -04:00AEPt@AEPt@APFN YAPXV2APrN 4APrV5APrX~~4ARTNASPFASPt(ASPt(B1Pt'B1Pt'BCTShBufTf NCMNT 4CTIDNCTNMNCTOwNCTTL 4}CpEPDATA@DATA@DATA@DATA@DATAf DATAf cDATAf /DATAf (DATA <yYDATA <yZDATA <yMDATA <yƶDCHTDSamDySN 4^Dye#DyeN4iDyeN4nDyeN4sDyeN4xDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3EVNT4 EVNT4FWO_GATCGTyp!!4=HCFGCEHCFGHCFGHCFG>>InScInVtLANE!LAST~~fLIMS!!4LNTD2LsrPaMCHNMODF4MODL3730NAVGNLNE`NOIS@lP1AMTP1RLPP1WDXP2AM`P2BAdP2RL\PBASBPBAS@PCONNPCONLPDMFYPDMF@PLOCHPLOCDPSZE`PTYPNPXLBRGNmNwRMXV1RMdNFKRMdV1RMdXF[RPrNN=RPrV1RUND  RUND  RUND  RUND  RUNT  #3RUNT  !RUNT  2RUNT  ! Rate 41RunN**NMS/N%@dSCAN@SMED SMLtSMPLYzSPACAfEcSPAC@|SPACAfEcSVER4.0SVER h SVER,,3Scal@Scan@TUBEH5Tmpr<UserphARphCH@phDYbigphQLcphTRphTRThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN YAPXV2APrN 4APrV5APrX~~4ARTNASPFASPt(ASPt(B1Pt'B1Pt'BCTShBufTf NCMNT 4CTIDNCTNMNCTOwNCTTL 4}CpEPDATA@DATA@DATA@DATA@DATAf DATAf cDATAf /DATAf (DATA <yYDATA <yZDATA <yMDATA <yƶDCHTDSamDySN 4^Dye#DyeN4iDyeN4nDyeN4sDyeN4xDyeWDyeW8DyeWSDyeWgEPVt!4EVNT 3EVNT 3EVNT4 EVNT4FTabvoFVocv^FWO_GATCFeat..v0GTyp!!4=HCFGCEHCFGHCFGHCFG>>InScInVtLANE!LAST~~fLIMS!!4LNTD2LsrPaMCHNMODF4MODL3730NAVGNLNE`NOIS@lP1AMTP1RLPP1WDXP2AM`P2BAdP2RL\PBASBPBAS@PCONNPCONLPDMFYPDMF@PLOCHPLOCDPSZE`PTYPNPXLBRGNmNwRMXV1RMdNFKRMdV1RMdXF[RPrNN=RPrV1RUND  RUND  RUND  RUND  RUNT  #3RUNT  !RUNT  2RUNT  ! Rate 41RunN**NMS/N%@dSCAN@SMED SMLtSMPLYzSPACAfEcSPAC@|SPACAfEcSVER4.0SVER h SVER,,3Scal@Scan@TUBEH5Tmpr<UserphARphCH@phDYbigphQLcphTRphTRXPvc.[.u2Zv&1x5roz;P= =&e=1&!rE_g4Y@D}4r(>z1{zK90plkm$48v3}k76gg`q:QS#tyYZ(WxdUo`($'tV&ShKn6[M36YZ_SGmm{2}3Nuo2Ko5ry 6CfWBOjzF4#@u]3Fo'h]oP0c{yl1}ssJGH"GrM7VpCJ]wQ4=AY+-^m]ZOQM.jCPf{w&BEey069>R/Ury&EHtVeXciW 45=)ySQbir8r|:7'I3#wor({Y]?\/t]66{qT[sEAhiW?WUBs*u $O6%nOp$lYTPSD`^BnBCmJ1y~I,YtR%g`+m6L#BlHz24a^jmJ!]`-;z128)TK7BKv'xPP"F77jD8z{V-AMV^@Yo]p&9'*z7r`%#3$fau?M`.4HzL}mbzee+n[Y<,h`Fzmj7a`<{!,8HW>y;`4E~G{WhcS]p?_sJOh^&6X`??}@8jbX-@^"qrB%P^gWTb*\mvH%lFDI| sYq-f^DR!_TcS{[}7&0E(_y"LAX;:#a92!ap(Z~~gln:tl|3[!]"oB/mn`kI Aq@E*_wPh,\1Fli|58S?'[um(]k@l'Z9dp"iK]jTj.uFgq%,NKo-jE(_y"%_oU@tC]2ZE<}|Uo%Z=r*r`sEY5_}4]'b5 ]{7HFwMzR93o^b,:V AmE$ww*{we*V&'Z9iS/oYFi$t.&"L4#,u6A|D2){|_nTJL:,aXj`Q Dx6m}>7:I1~8U'W33EZ'nO$`j|"/WQ`Q`zX>ykI0o6Gzv&1NmbcnP_[O~}<"&eG1<@O(LxCmpD0,_03gPV]O8oe])%|2CWllme[OCy KJFU'.QHT/}]66{6sA bBT9zH'zeE^O{U,\)lOY%}WeoVL6F-{h(0+5?(,wg:?LB\,(CW22Q&]Q#V>Ax7cXF$~y;|>EY|3.B|ux).u7z"$-m8)FTk2XS"j+OC5wz^`Mv,ZD-_p;s_|g+V3SKo)67:I1nK-k]|sOJr4{6QuA!9@1%ilJG82G [7P^E$62:gtPques$y"EV["9CY\IVys<7qL}4|MdwYc_`k:!.tko2KWtAcP@>/D?*Q| -4KD@4ir$G%1` b&}3672tX>VH~?p?}@8jVFTm[Po)9}P5O-!b+Smfc4P8U5ZfoQe=N^tpups'Mvn5mr+8/Yb~;HR;L>InScInVtLANE!LAST~~fLIMS!!4LNTD2LsrPaMCHNMODF4MODL3730NAVGNLNE`NOIS@lP1AMTP1RLPP1WDXP2AM`P2BAdP2RL\PBASBPBAS@PCONNPCONLPDMFYPDMF@PLOCHPLOCDPSZE`PTYPNPXLBRGNmNwRMXV1RMdNFKRMdV1RMdXF[RPrNN=RPrV1RUND  RUND  RUND  RUND  RUNT  #3RUNT  !RUNT  2RUNT  ! Rate 41RunN**NMS/N%@dSCAN@SMED SMLtSMPLYzSPACAfEcSPAC@|SPACAfEcSVER4.0SVER h SVER,,3Scal@Scan@TUBEH5Tmpr<UserphARphCH@phDYbigphQLcphTRphTR