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                 # !  !&(  *"(% #'      $($.)#  &       $$$0     ) $#                 $                                                 #     "         %   - "    $ (   "   "         *        #' '!+%  %('""")%'$  $&"# !"  %#"#%$8  &  $!        !  %    2 "                            2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 8d2e4d8e663d4d2fb1e2ab36a2b47aeeSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_G4_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z%3Hf3BIIIP`|3LZ\YZj/3,(3R#-.*''*,,*''*030# *GWS=  -HUN3 aF3'"fQ@0n_P>)v]MGGHD8"vqrx|toligioyvt|z~n]PHC<0"      (39:742232.& "+//,(#  !! 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'66W+E,V d*1!4I`:FE^U d\T@J]+S\@P"KV<5-*$AQB)92:YN"* -?Q.';!=$Q9Y>O3+*:ECMJ# d Y0,OY52 F265<W>2/% RFGD@ <P-cEG P*K;2W'?6A2 D.E=2-_bU@29b,_#-A?E 2&+BC8UGZS;.K2'0J:\ C)6"8VC2!TZ+_BUV TT_ '!H 2YC2S%H8 C$)4"(H; P'D@ #<h]*a_\Xf'*<:6{S^/;O%6y]ED <R.3^Vc~.w(/G?s)iFk9O{5YRhG_ 8qQ b}[SKr~jmae1ny~e#@  U9cS3Wz(}WP+mhdF4E#9`ce%D 7MT\^w G z;Hy9gjoejz<LB`[^6HiGG<c! @q2?Rp=J0+@ tRwg6P7~THk=.lKjZmSQ@'jQ\laamD#@.@9d`}k #d ;K/:8iVzNQ{0V?Ge&  U3YD[  ? fFv+tA'\VbX{8[JZA08o<k(38J{>>G8?nGC6C;v+AP<R7==<;>2.!)j+&MY)G7OF(,/3*G-)+P!(,0R=--0.'XO I\qVgqV&+`\ Gz^ \S-tpSx#8Q0L7Qx<'9H(0;4s\!V58 EVn5 +% W.FT]Gh3.Zs =' 6:,_"S)uOBY8* q4/9]^ k7MTg(pd1@8 &J('L=$-Y \UNF]*.";oSR@N5'ch83>>r(=B1r\<g>>77777000<(>000$97,9T)TT9JT7TATTb$CU67JX2T27\bTT7)7w9JHSS,S4OLZ,SO'3TBy,OOOO,OO,OSOO,oo;oMMMM`MMMMMopMooooMhMMMMMooooFo]_?IMMoMfMMMMMMM`MojMrD(po"Aqpp=p8dpN E#uhksEP_var8sJ5^,n?VU,#dLpNw0$hRpA91px,pvetdE(8^tPdGx[h[={-PmW"Qpu)l:p8e8H" dCEr\,,mvdPtp.,s +QpdE{=uRvppgdkwE 84f>XN8^pMX8|w!86p _H  ?7H=ed,pg>^p7G{` 7Ek B8$3tTh+2:Zc+=]zd9,>=z-eg pGF}[F( ~>MkTmsF!z0)sF`u7Tod';ueb oHh)*vL|6p},#[M7]6=g1]CJa eB%mE79m@EqL+O2a* >WBjVcBW)mU6k1hMY%1LWZ_E r Zo8?,<(zWsk_(0:pIlW2E[">G@\k78Irh!k*p?C~L<Z 4Xn^@H2ri0>}` #eT+}[>3g6vR GCAAAAAGGAAAATAACCCCCACACTCCGGAGAGCAAGCCCGACAACGCGGACGGCCTCAGAGGCTGCACCATCATTCCCAAGAGGAGAGCGAAACTAGGGTTGGATGGATGGTCCTAACCACGGAAGGCGGTCATAGGTGGACTCACCCTTTCCAACCGGCCGGACAAGGAGCCGCCGGTTCAAGGTTGGAATATGAGCCGCCCGAAGGAATCGGTCGGCCTAAAAGAGGGCACAGAGGAGTGGCATTTTCCCGAGACACCTTCCAGGGTAAAATGAGGGGCAGGGGGGACGCCGTCTTCCTCAGAGGGAGCCGAAGTAGGGCCTCCCGCGCGAGAGAGTTGGGCTCCTCCAACGCGCAAAAGGTTAAAAAACAATAAGCTTGCCTGTGCGGAGGACTTAATCCACAACCTCTAGTGACACGATTGGTCCACGAGCACTTAGAGAGATGGCGCGGGCTCCAAAGCATTGTCTTCTTTATAGGGGAGCAGAAGAGCGTCTAAATCGGAAAAAGTTCCCTAGACAAAACTCCGCGGGGGAAATACCAACTTGTAAGATACATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:21:04 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTS\BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;w6TDATA ;w6DATA ;w6BDATA ;w64DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~[HLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS1rP1AM1bGP1RL1bC-P1WD1bKP2AM1bTP2BA11YP2RL1bPSPBAS113PBAS111PCON11@PCON11>PDMFTrPDMF1PLOC1b:iPLOC1b6PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%1jSCAN@SMED SMLtSMPLT^SPACMSPAC1SPACMSVER4.0SVER \SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCH1phDYbigphQLcphTRphTRTThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTS\BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;w6TDATA ;w6DATA ;w6BDATA ;w64DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabk+FVockFWO_GATCFeat..jGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~[HLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS1rP1AM1bGP1RL1bC-P1WD1bKP2AM1bTP2BA11YP2RL1bPSPBAS113PBAS111PCON11@PCON11>PDMFTrPDMF1PLOC1b:iPLOC1b6PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%1jSCAN@SMED SMLtSMPLT^SPACMSPAC1SPACMSVER4.0SVER \SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCH1phDYbigphQLcphTRphTR-"3oAZKC[p3}$r&kUV'4Mu*}c+-O)C@5&J06THKoS]/~8R&}[>FESt%e]:aH<6.tJdQeK~jca1n6*X!afT7+|4C9|z*yEwiO*$Fs{`jC52#GN;Fi$t.&)`|W"[XYTZ`/6?|$lQ%e+Bw9#MBz3)-I5d3g9kw6T-(WxdUo`($'tV&MWW|;WL.]_93Qq[%w%:T?NWFikas1HX.M;76N8{q6.>`AJP:zdq3+fyW\j&1qTFU-=DaJ2`>nX$t!TQqvLWja$~,Zv{:LByB+:iwE9;lwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX6jJ}Bq#pJE8m+adJs_-ZZ^4n\809b#.Igl0eY?R08o3L"#`5E^W6t{_"#k6l2WePm6W.#oU4v_DwW>qO9`}!&RW8X&tG98PbC.Ju5%2-8~$GPf$}pG}3G4RF~&b+&S)BcS[7}?ef5U_9}j~:HzZbti2]2rRU)>`V|I+!K|-o_}4]'b5 ]{7HFwMzR9vAMV^@c O*Pv#$6wC(vJKq&1hE;qT%;5]U' S!oSRIoM/V/5`agvb_@U])%,1+7XM&b$SXtmx+9Hgn<_swW^$beP`C:F__diM(]y"/:Boz?&qNR0Xwt2)lOY^k'eaK|`Bt#2ze/z,(,y]18`F7IOo_3@/C 4O$AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtAUDTvvy>B1PtB1PtBCTS\BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;w6TDATA ;w6DATA ;w6BDATA ;w64DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabk+FVockFWO_GATCFeat..jGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANELAST~~[HLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS1rP1AM1bGP1RL1bC-P1WD1bKP2AM1bTP2BA11YP2RL1bPSPBAS113PBAS111PCON11@PCON11>PDMFTrPDMF1PLOC1b:iPLOC1b6PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%1jSCAN@SMED SMLtSMPLT^SPACMSPAC1SPACMSVER4.0SVER \SVER,,/Scal@Scan@TUBEG4Tmpr<UserphARphCH1phDYbigphQLcphTRphTR