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                                w                                                                                                                                      2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 2ca02e665e494a309103b6b62c9a37d5Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_E8_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z &Gf} 1Ndy+Y"Ju  ,;L[fhbUD4%nV>(+C[q}o_M8!kCtS1 \3uia[WTQNLIGEC?;731013552/*&#!           !#$%'+/5:>ABDDDB>93.*)+/7D\{_v)0 ~WEFY|M' `H}(}E"#%#$(++*'%"  "" (>c0*a}xS^oA%  %,26751+$"=n"47nnR( -L}v$XgN^nC,"!$(,037;@CDC@==BQjGM0<ywL>kp|7   %T6}{6_2 6[ lE( !""$%&'%"6h gOc1-|b}8    6^i@!    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BNRdFL*#7# 6Pa^B[ 1_8^1'JdJ)S(.*3[]*Q\ &  2]8L "\^;UR>Q,: (TB C`/Lc27M R654Jc :; +63&-Nb&=%6(bDPc9%JCU'U\dU$+/8O3;P7&L *$[ -\>=J(P1bU: Q/ ,/T<K^8$D  VcAPDW6;+UQID;GB`b#!#G4X5C!G0ECZ8O `Tc (,\FF%:bBF8@Z-.R[Ib,(= N" 9(DSU<%) F[E!..:1!JBA@b U2*H9 (0' ">W2T[6;2OM\W$DM;DIWQcQ69>[P'OE#7[4%24 N,V/1/':$B2_OHb2#a22 -?6;=9)T,NX' " S2Y6gb$p6}_4,<MkZf}$OL~P0oz~\4A5j:$fM_KI b AJ#BK e.\UIGm O] glbbr/S+.{@Z)9K$6w L{)PamK!H E/`]Fb$> X{K.T/CeMWT'T&^#\J 1$~,mo%N CJ1&j-h9e5 D{%;x1^ gXV (HE YFE'b/w8ICtp*Z >-93l Vz ^s][\UE!r>|K%oO11ZHfkm3{QoX$ (3`, |y~M[{3byu2nOkjMqd=WU5" uTzCQ dD(H0JwAx'b^6Qi3\5>*f>]qr`9+;LB ~sge ! % H W _mJ TK `$ 1roWcXUVNZiZaF2-61758G1(!4*+(-32! %(3           %!               8  (2.  # !%(!"- %:" )(*+;"-(1/+B )!!09+,.),,6D!4A/1?3=#41&4)0%31-:>? &JAj++JJJUJJJJTTXImGO=LLfH[FCPiELbEf`cYU;]U^jORZccwwwfigD|eh_}vkAAAwd^ilAAZ=vM~l{yf~Tms]jOnvzzYozzzzz!*zzzzzzp-pTp*poP+p8,#pzohu8uKI/R0[>cuSC\UBdo>d[,?d"4;pk ,)OCdG,{P3p&gD ~YIz\Od4\yV[8?rp\?8!b.i ,J8p9|F/7O~Tb/9yPiV 9YpE+,gd u2afCiTX8SppppRI,{ppps{qp0ApG3q9pp?0gu)+>_L9$KZ+{mx49Ms';42M@B>.&$$]x$#D"e0O|!3LBD%6'1G!C#3*8+@Z#/+':132FC6* 75J.#0:Cj'2AT-/ /LB%X 6I4,9&8;;0? :=+(:-2(!24fJ '"%##$&"4H&6Jy"96J&?'$,!<P17ApV'K7&3G1A,*1&%#0@2"&;#@("jp/F,&(!< &1$1& >c #60"97Oh5X!<;'20<2o9>(0>#)3 8!D"7=!E-Y4%<"BJ$5;$!(*60;1;IOLRm!#8[;/</',?50C/=/45.)%,HS#%<;:*/08BJd /=FO(H4"@Mb;:Q=!R#=$'P$/1%69Il`OH$ ,p1>/OE-$AMacA Cn XFI3.T@+WK- (X4 c(7;|*3T5J6=Sj8Nd/8sRbsO3I/Iul:pY?Mog+]Kp5(XD58j3_euiJA&G)UO^]kO<LW`>V:wQ?w^MwH]?C&Uq4HFzgHlsx{^"R@ kQrTAAAAGGGGGATAACACCCACACATCGGGTGAGTAACCCGGACAGCGAGGACGTACTTATACACCGCTACAACATTCCTCATAAGAGACACATATTAGGGTTTGGTTAGTATACCAAACGACGGAAGGCCGTTATGAGTGGGTTACCCCTTTCCCAACAAAAGGACAAGGAGCCGCCCGAGGAATTTTGAAATAGGACGCGCCAAAAGGGAAAGGTTCTCCTACGAAAAGGGCGTGAAGTGTGGGATTTAAAAGGCTATCACTAACTTTAGTGTTGAGGACGTGGTAAAGACACCTTCCTAAAAGGAGAGATACGGAAGGGTCGCCATCCGCGAGGGGGGGTTGCCCTTCACGGCCGAGGTTAGGTAGACAATACATGCGCCGTGGAAAGGAAAAACCAGGGAAAACCATACCTGCATTTGGGAAGCCGTGGGCCCATGTGGGGATGAAGGGAGGAAATATAGGCACTGCCTCCTTCTTTGGTTGGGAAGAATGGTGCCCCGATACTATGGACGCCGATGATATAGATGTATCCATTGTCAAGTAAGCACCTGTGGTAGCTGTCTCTATATTCTGAGACACAGCCTGAGTGTATACCACAGCCGCAATACTCTTTGAGTGAAGAGACTATCGTBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:21:51 -04:00AEPt?xAEPt?xAPFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?TTDATA ?TDATA ?TS:DATA ?TҎDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE8LAST~~LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMyjP1RLyeP1WDyoP2AMyyP2BAyy~P2RLytPBASyyTPBASyyRPCONyyclPCONyy`PDMFTrPDMFRPLOCy\PLOCyWPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACAb'SPACQSPACAb'SVER4.0SVER SVER,,/Scal@Scan@TUBEE8Tmpr<UserphARphCHRphDYbigphQLcphTRphTRmThis is the confidence range *ABI_Limits..FVocAEPt?xAEPt?xAPFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?TTDATA ?TDATA ?TS:DATA ?TҎDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE8LAST~~LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMyjP1RLyeP1WDyoP2AMyyP2BAyy~P2RLytPBASyyTPBASyyRPCONyyclPCONyy`PDMFTrPDMFRPLOCy\PLOCyWPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACAb'SPACQSPACAb'SVER4.0SVER SVER,,/Scal@Scan@TUBEE8Tmpr<UserphARphCHRphDYbigphQLcphTRphTR-Dq.$+W x:H-p9bgh`U*lO6viO$@I{-!`:ZPV2fI&$yNihB:Drj{H,:MX]#=c$DfqcdsTF]luKgk]35FQ6nB!X`7.w>6?xHf.QR9JK[YlyjSu30&r[AgC3p`giE$4~A8./U=f:hN{+eqL}4|Md'ac_`k(U.*C]J=E]1|eKW3CNIG2gbXW,VE+0&'7%4X+*fl979v$sp3@=?]p&q!R;{?kJ:h+p?H(+Q;T`h3/[ze/G,y"o-,(B~b) e:=8}R"wKgpjm2D%~4r8h98&>LbsMvF(wD1RuM]\*+x=osrsd45x~;ABDD2K#Z]Pk=o?eU-:.<)%,8#V>Ax`X~lTmt.qK|-o*.AQ>UA!%EeKOWQ`QV-!w2l89+uTE#!#hmmfc4hAo?e3{0+oiw87[%Vr6R[Ule,rS]RnjH,3LiPtj glX ,cW`}F)h>?[ha_DMOFU>^[?|&Rv3672Z)~6bI.J:*yEwiOM#Y\V&&OE6OLogXY@ZB 3O{ ~VJ(/on}sF{AR8=(V-OGC#!^MrrGqqQX8Bv):vZ`nF+DweczEI"m.9,(B~b) e:=K{_"#k<>Dj&['K2;+hj3jT$3o%+0Oj!Q4]"{vz6$l>o XS_d.u?zD6~hlwXs9?Ou4FS7&3-OT5ro{`N%7`h?&0(B~W^17]GhnW"K-[llj_odF4NYHY8|!I,62Z5hf6]X?K90j2oFtZ[pZX<-KGzwx?Qx]c){-*<\m(+<-F3x[R2LxC{IF}o-8xOrz6 ?63_1bTio0>InScInVtLANE8LAST~~LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQP1AMyjP1RLyeP1WDyoP2AMyyP2BAyy~P2RLytPBASyyTPBASyyRPCONyyclPCONyy`PDMFTrPDMFRPLOCy\PLOCyWPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACAb'SPACQSPACAb'SVER4.0SVER SVER,,/Scal@Scan@TUBEE8Tmpr<UserphARphCHRphDYbigphQLcphTRphTR