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" $23!) *6-:+<2"%   &)'.,&1 /!02/='$"*.   !&1 "        &      #&   !$D """ ) "           ,$  (" " $,&'$% %)&"" *'*!'"  -     # *+' # '  ,*     % "# ))+.2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f676367759d1403eba35e6b7377494c2Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_E5_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7zAf2^ ;m$Q/hFu 6Schfa`dmxu^@h8^0iH0" $Ag )ASaks{seWJ>4) vV5l@\+wO, }zxvspmmoqssng^XUUY\_`^]\^bjv!Fo+@LQL=#m<vcSE:2+&#! !#%&'(*,-/26:?BA=5-'%)08=>:3,%! !#%(*)(%"!""#! !"(.4:?DIMPSUWY[\\]^_`^ZSI?76>RwnZ hxG>"7EmT~lel`jW[ rF,,Gn&lS W1   '7V$UKs=    '*(!  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X.(@Z$ $, N?D_^^]\\KZQ R/?$-";PYQX0J3 *238 _ )O#$9P0M)9<:+049N- 4@QK dG-!9SC13] `Fa*6DV.$a9WCG-/6U 2!#+1A 3#+U%;XZC^IMUIW -0=3LBPDVPA=A>A:c+_C 9&BP.73+JD NUE*V/E<2P9+VZ>LK:^a;)I5Y4:*K3C V[`:U"GC^J2Q+\S(8321+9G32 &DYC.L3"BB"=NRIN@X\5MBY "- "\-C\ XBG V2A%?G02!8HcVGH@V Zaa%C!.?NV"V 24cL42+2;C4LC10!EYPHM6.%.'GH62W6c2][%H-6 GH YD 202B .LH2\ 25.M.bcJ\ %,WJbO;$-(7O]8+:08 G28W:Gc 2,`PR,(Qb@A(^ Gd$ZU8N8<,!(8;5A%(88_"J22,^b& DF,/4I5+ =j[95zcp<3$=sUH &gNMR1{X+7JT LFo +1 fGv*arn. ^Rf7nCaw")q7A-ht,]2 $d f_c: LrJ\Bb.JHADWOIJdv4u , s^ iSK;H*S}xB6b[k? # ;$wPp.u_(4Q+.J_OG f6pco`&:Mv@'Kka&O0@<ab'?NU'[W0K1u']+zvg *e<LJ:@y2+Z2uNYjp/K7] ,D)>vt6szxxwy~nl?]q;L\jkg!y$U qGuVb!Hp[A2ojFo !;z#}Q=nG4O}C*-zE-Us 9q|-sZ&ooVb FSS.]epu|  SG/ ;sBx \[{* RIo (&l>v:i[7(S$'@Hl,B8E7"{ M }6DoM)+J6G.^@ (K'\J<9ONxLxben$]~zL0 rZ4*+ Cqs=, u!_\-~Lk;\|4k^' Aj^ 5"; ` `gf2LDZb55m6WRRNMC# 8"/?G&-  &  @]1.@ xx(   (!       8(  "%    %$:%+  $!3! &/*'/0*!5=;"+,#1B**$6$(6)&6666;66((-,K+*.69,=7E)6:-,K$:F06=HU8;.>6DQ0U-DIA@P[?GW]WWWW[ddGMTgzrhMgLHGeydRXY?cU^]]ZLVmwZaoZCtljcvgp]]]s]bbgqsudl}}g}n4 [~pppppp5 <b```m` ``uuu uuu CCC  CCCCCC  C      <"x""<"<""xx<x<<xxx""x"x"<"<rVrVV VVVV?rrrVrrrrV V rrrr    XXXXXXwiNUX.XXXXXXdXX$,Tw peuhtcwpz0^nXA*b\T*pl D<`{0pN k_wuo]OugDtR,sekM{p1G pm84XAl#{J<6s`Q CYUg>Za+%#+ ^b8+ wP~2 Ei7EZ2G,t8kkn#8F\38%v&Ep28su:4tp~gQ{A)*)pmWO9_ 2@pv)p0=xQp;,, pGQp1dp-3Ip4p pdzd88pp8+2{pppippFp8m,pZ(p,p#ypu,pppp-8 o\\8p,>DX(J lN*`m~``!;63@6\4-)Wt*]Z& %(5'$ +)2L\M(*7)!/AK3&%,-,'7+## *$,1[e)Fc;6[7K/&#.GA=>31i?3H**C O6&345& %E^ $4&&$" (2@ !+4o`?H]DV;+ <G$,!&/%5.' .]U=;6?!$,'4+-+ -'.D"$,hX>*(;)/+ .3.-D6$8fH:>C-D>cF?'ELH7!9;!;,B/*-71RK""/TB+=5)AMD\%,2"/F8>!&E'!P7:J >$=HlTBCU*)-W*$%:"5D# -!/HXH"6=ODC'E)67Q_E_4&710P0,I)',3@'DK;;G:0)G59((S=6 S:%M#A*/XL]P'M^#L-$'!E@'M!6B+ID;+`OYb>nA</EmO_Dt_QDqN#F?z/ullR9z.I7I2%@E|9;N539QF}!GPiJQgK){TcDi^&PjrI0E6HtfSeUUQxL7I'Jaj\:h7zU=ssRB]ZtXgsh  2pP` P.kz.@zAa"HbDOTneVY,FtHUB pBEu< q"AzG$y!0D);r 5sfCz> :8>DH5+VG}w RRLl`ou{ow8~7AA2@QR{f{E|76AFAe'35}?a/!VV"ut0:< }zX)(_f^K Rw"x|MIfp56Pt29B/!MnbZESd-t0mrW6n. !TGAAGATTTGTATACCCTCACCACATTCGTTGACACACCCCTACATCGAAGACACACACATTGGCCGGTCATACATCCCCCATAAGAGACCCGTTTTAGGGTTGGGTGGGTGCGCCTACCCCCCTTAGAAAATTGTGCGTCCATTTCCCCTTTCACACCAAAAGGAGGAGGACCCGCCGGTCGGAGTTTAGACCCTCCCCCTCCAGAAGGGGTACTCGGTGGACCGAGAAGAGCGCAAGGGTTGGGAAGGAAACGGGGGTCCCGTATTGGGAAGGAGAGTTTGGGGTTGGGAAATCTTCCAAAACGGACCGGGAGGGAAGGGTCTCCTTCCCCTTGGCCGAAAGGGCCTGCGAAGCCAGGGGTTACGAGCTCGGAGCTCCGAATTGGCTAGAAATAAACCGGAGAAGGCCAACCGGTCTATAGGGACCCCTGGAAAGATGTAGGGATGAAAGGAGTAAAGGGAAGCAATACTTCCTTATTTTGAAGGGGGGAAAGCCGCCCCCTCGATAGGGCCGACGACGCTAGAAAAATCGGCGTCGTCACAGAGGAAAGAGTGCTAGTTGACTATATATTAGGCATGACAACCTAGATCTATACCACATCCGCCATTCTGTTTGGGTGTATGAGACATCTTATGGAACTATTATCCTCTAATTAAATCCCGACGCAAACAGCGGTTTAATGGACAGATGCACTCCCGGGGAGGGCCTGTTAAGGGGGCAGAACCGCTGCACGGACTAGAGGCGGTCGGCACCGTAGAAAACCACAATACGTCTCGCCGACATAAAATCGAGGAAAGTCAGCATATCTTCGAGCGAGTCAATCCTTCTGAACGGAGGGGAGCCGAGTGTGGTCCTTACCATAATGCCGTACTCGCTAGAGTGTTCGCGTATAGAACGGACTTCATGTGTCCACAAACTGAGGAGTATACGCGGGCACACTCATATGTGACCGAGAGGAAGGGTGTGTCTCTAGCCGTGGATCTCTAACACTATGAGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:21:30 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt ASPt B1Pt B1Pt BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?ZTDATA ?ZDATA ?ZSFDATA ?ZҠDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE'LAST~~GLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMy1P1RLqeP1WDP2AMP2BAaP2RLPBASVPBASR5PCONmPCONiPDMFTrPDMFRPLOCaPLOCZPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACA`ϻSPACRSPACA`ϻSVER4.0SVER SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR0phDYbigphQLcphTRphTRwThis is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt ASPt B1Pt B1Pt BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?ZTDATA ?ZDATA ?ZSFDATA ?ZҠDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab+FVocFWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE'LAST~~GLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMy1P1RLqeP1WDP2AMP2BAaP2RLPBASVPBASR5PCONmPCONiPDMFTrPDMFRPLOCaPLOCZPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACA`ϻSPACRSPACA`ϻSVER4.0SVER SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR0phDYbigphQLcphTRphTRA08^#5E^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]Z>i4Fzn&\c35|=}hy6Oe:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYX[l'ny#/D?*Uq$[-o9D4oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPt ASPt AUDTvv>B1Pt B1Pt BCTSBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?ZTDATA ?ZDATA ?ZSFDATA ?ZҠDCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab+FVocFWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE'LAST~~GLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISRP1AMy1P1RLqeP1WDP2AMP2BAaP2RLPBASVPBASR5PCONmPCONiPDMFTrPDMFRPLOCaPLOCZPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QSCAN@SMED SMLtSMPLT^SPACA`ϻSPACRSPACA`ϻSVER4.0SVER SVER,,/Scal@Scan@TUBEE5Tmpr<UserphARphCHR0phDYbigphQLcphTRphTR