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"(# , ,+  "  &                '        !                *                       !                                                                                                                           2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: f8765e33768c4c638291c19c92eca1baSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_D8_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z%/@]*Sy6fDwBd{D(f@[jhQ+J  b2vE.4JaiZ9m/pVRZ\L#['~S"u]MA6,}sib__cjorncR>,!/Hj];)*:LVO7  /Yc>ucXQLG@7,  (6>=70.5E[sb^]ybf(`&$kNx]yYjCBvW?36DXn jM00I`py}}|zxtolq\Q3{ZMjYRT_m{ c} vm qZJ@:9;AIS[bfjoyA7A]SDhL;0)$$.J}0#1]e7IB+K0]gqy2 %F;Nnf8*x; #&''%$$&,5@N_v7vy6smrxpjo!28Ytgs4OmxD*"!"&,4;<7- %6FOND4#&'"    $'+/5=EKLH?3'!%&# +5;=<=BIQWXVSU]l~Tvqsxf[[fuCvKqddimonmmmmje`[YYZYUMD=;?FKMKKWy}CvzMk@03@MTUPJD>80%  %,+$      &'""#"! 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"#!   +7=>;51037;<92)   $+00.+)+07>BB?81,)*/6?GLLG>4,().5:;7/%              !!   %)'"       $),,+(%       !!$%!                         $((#          &)*(%#"##!",23.&%,0/)!    $('# $(($#&'%"#()&   $%$#"#$$!                                                                                 !                                                                                                                                                                                                                                                                                                                                                                                         #(&                                                                                                                                                                         "!  #()%      1@OvAA` AKB.bcpKB_3730_POP7_BDTv3.mobtermGGGAGGTCAAAGATAAAATATTAAATCATCATTACTGTGAATCAAGTCCGCCGCGAAGGCATTCGTAGACGGCTCTACCATAATTTTTAGCAGACAGACCTAATTTTGGCTTGATTGTACATCCCAATAAAGGACGTCCATATTTGTTCAGTTTATCCCTTTCGACCTGAATACCATGAGGCGGTCCGATGAAAGTTTTGGAATAAGCAGGAGGAATTCGAAGGTCTTCCAAGCGTAAAGCGCGTAGAGCCTTAAATCCAAAAACATTACCTACAATGGAGGTGAACAAGTTGGTGACGGAACCTTCCTCGAATAGATCCAAGGGATAAGCTACCTACGCGATATATTGGTTTTCTTCCCCGGGGAGGGGTTCGATGTAAAACCACCCCCCCTTGTAAAGGGAAAAAGGGAGGTCAAAGATAAAATATTAAATCATCATTACTGTGAATCAAGTCCGCCGCGAAGGCATTCGTAGACGGCTCTACCATAATTTTTAGCAGACAGACCTAATTTTGGCTTGATTGTACATCCCAATAAAGGACGTCCATATTTGTTCAGTTTATCCCTTTCGACCTGAATACCATGAGGCGGTCCGATGAAAGTTTTGGAATAAGCAGGAGGAATTCGAAGGTCTTCCAAGCGTAAAGCGCGTAGAGCCTTAAATCCAAAAACATTACCTACAATGGAGGTGAACAAGTTGGTGACGGAACCTTCCTCGAATAGATCCAAGGGATAAGCTACCTACGCGATATATTGGTTTTCTTCCCCGGGGAGGGGTTCGATGTAAAACCACCCCCCCTTGTAAAGGGAAAAA ".;ES^isy  +9>GT]fp~)6BN\it (3@LXcmy)4?JXbn{ #/;GQ\fr $0<FR]jv)6CO[gs~ $1>JWcoz  $ 0 < G Q \ i u    ) 6 B M Z f q }  # . : F R ] i u   ! . ; F Q ] k u    & 3 ? K W d p {  %0=HS_iw*4@KXbny $0=GT`lx -=GQ^ix"*6@JSajs ".;ES^isy  +9>GT]fp~)6BN\it (3@LXcmy)4?JXbn{ #/;GQ\fr $0<FR]jv)6CO[gs~ $1>JWcoz  $ 0 < G Q \ i u    ) 6 B M Z f q }  # . : F R ] i u   ! . ; F Q ] k u    & 3 ? K W d p {  %0=HS_iw*4@KXbny $0=GT`lx -=GQ^ix"*6@JSajs    #/)# %4#  )8>8>/ /,8+&38"2&$++&#"&%#228828>6616600;11;>;33+8001)" "1;$$(1;31;>;;;>88;13;;;>8;86668;";83...$"211,#6.$0#228>>2>1;6-2+8;888%;++"18."261386#>828+3+331!"#("+"+"$+.)".";.28;311;;;11;;8..&1+..8&$"0"2.%++"228211+  + ""-     '--  "            #/)# %4#  )8>8>/ /,8+&38"2&$++&#"&%#228828>6616600;11;>;33+8001)" "1;$$(1;31;>;;;>88;13;;;>8;86668;";83...$"211,#6.$0#228>>2>1;6-2+8;888%;++"18."261386#>828+3+331!"#("+"+"$+.)".";.28;311;;;11;;8..&1+..8&$"0"2.%++"228211+  + ""-     '--  "        N(; XN<)LB)2L0%KT .YB1((\5CE$ Q+2GN-B: SJ'` ^! !5#''$:UaM,<+`5 .`SDX>UGYJ U<*%CQ!2SQ33>Q2:<[BHJ:4H@)/:!$.E? U7S"D!A]HY'9?6?M>Z`RCT^+L F? 6GH'\*6!WQ7:#0 C@*G%3^&D%<45+2Y; ?' ^INKB7:.21MC;<'D N2W+L>]5)OL2=R3C ;"?_-ZJ2?74)\!_2:`E( 7%, F+2&b WHJVP0VV`=(O=]7&Wb*=C4>I 5" P56ACSA%U8 @?NIPU+K 9K62ZKVd_2N"5TE6,3c\>7bU L6- P (ju>H,i@:.C5I7PVbsLHOmKjH+0 q;&+my2?Y,4Qgy{T#  j3!;<Plb2Sp$i`>L.oQd6F5_BH&z`@]]mqpb X/;:^P# `El<F5 x;] <XOf M<;]^#Ga4yp7YGaJN$r b<}+&@kq{xwaiFD}owz  ` `~IdWDDez,Z/[G1lLz{5'6 I@/.' -L a2%+t<         ( $  ( &!09   $  3.@e`*z?N 6S@J, ic\+EBTZ0Aq9!p(*'  ''''''''   }NWU| ,,I d)\p\$, Vm9#,=a_Ko%n  d T|+x k,8[Vb88> 0XE, Re@08I,FXzV ??M @vx3,=r1l1V(9.cd>&Qb!(8m jUo #R{}8{8.wAHd,8]&}Qp6B #<( H(  CEu  &Q2Q>CoP-B0el`w^;TI1mwzjo.4Xk\LO$9>p3%:=j=L_Td2itSs,UzF\7?.} CA4_J0(Dn>3rkWgcYW=<ci\QH,7/6p77em4YP.qjF2 +}&T?L"WKygb,{`\#lc3<l\lg|h};p9mey _^[qVr^{nI/(/B6)6>*x>`Mq=N~uiIEzr }Er-0a@:^Fe#kU +TAAGAAGGGGGATACCCTATGATTCCACAACAATTGTGAGGAACCCCTAAAACATGGAATTACTTAGAGACCTCTACAACATTCCCCATAAGAGAGAGGTCTTAGGGTTTGGATGATCCACCAAACCCCGGAAGGCCGTTATAGGTGGTTTACATCTTTCCCACCAAGAGGACAAGGAGACGCCCGAATAAGGTTGCCATAGTACCCGCCCGAAGGACTATGTTCTCCTACCAAAAGGGAGCGCAGAGAGGCCTTTCAACCCGTATACCTTCCACTGTAGTTGAGCGCTTAGTTGACAAACCTTCCTTCTAGGATATAAAGGAAAGGGGCTCCTACCGCGAGATATAGTTGGCCTCCTTTACACACCTACGCTATGAACTTCAATGGGGTTTAGTATTCCAACCCGGBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:21:53 -04:00AEPt>AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTSqkBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?8TDATA ?8DATA ?8SDATA ?8:DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE:LAST~~oLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQzP1AM.aP1RL.^eP1WD.dP2AM.kP2BAnKP2RL.gPBASSDPBASQPCON\PCON[7PDMFTrPDMFQPLOC.X PLOC.TPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QrSCAN@SMED SMLtSMPLT^SPACMSPACQSPACMSVER4.0SVER q`SVER,,/Scal@Scan@TUBED8Tmpr<UserphARphCHQphDYbigphQLcphTRphTR62This is the confidence range *ABI_Limits..FVocAEPt>AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTSqkBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?8TDATA ?8DATA ?8SDATA ?8:DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE:LAST~~oLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQzP1AM.aP1RL.^eP1WD.dP2AM.kP2BAnKP2RL.gPBASSDPBASQPCON\PCON[7PDMFTrPDMFQPLOC.X PLOC.TPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QrSCAN@SMED SMLtSMPLT^SPACMSPACQSPACMSVER4.0SVER q`SVER,,/Scal@Scan@TUBED8Tmpr<UserphARphCHQphDYbigphQLcphTRphTRA08^#5EN^D\we*S#ibu@Qe7EG>x'H1PSpj:6R}ag5d9w];H:'~%b9DBjh'&~o[k7MLr92U*a!ZCCd+4ko)Yr13.iOW/D-]$#{N.g\7dHm,_~/S9D>)ecH<]%9t}TU}xE_q-%Qvqs@dUa_e^W^4c Y \,yvMU:6%4sI}]66{6sA bBTd.u?zAw3+fc`]Z>i4Fzn&\c35|=J/yh++:!VO_5M%_|_n?NhN{C]J=E]V&=o>ZEZ,*`y{XW,@|DmOYX[l'ny#/D?*Uq$[-o9D4oW~!^F7N? >S]T'_C:[rn{.QHT"Hf.Q F3eG"k@j/.3]H6X+9mK\TI_aEdO{7+VfU~oM6CqwToN668d#AEPt>APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtAUDTvvB1PtB1PtBCTSqkBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?8TDATA ?8DATA ?8SDATA ?8:DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//GTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE:LAST~~oLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISQzP1AM.aP1RL.^eP1WD.dP2AM.kP2BAnKP2RL.gPBASSDPBASQPCON\PCON[7PDMFTrPDMFQPLOC.X PLOC.TPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%QrSCAN@SMED SMLtSMPLT^SPACMSPACQSPACMSVER4.0SVER q`SVER,,/Scal@Scan@TUBED8Tmpr<UserphARphCHQphDYbigphQLcphTRphTR