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'                                                                                                    ,,@II^^^{{{&&&999DDDXXXaaavv      ***333GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR6LQSSTSTSSRQPOOMMKKIGGEDB@?><;:87533JKKCCD_^_uuu  &%&---545<<ABBIIJPPPWWV\\\cccihhnnntttyyy~}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: 3ef556f1c31749389a6b90b54d169413Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_15-08_18903130Seq15262131439_sgoyette_131439_628Fmfagan96-WellK1344_C12_ESRBCL628FKB_3730_POP7_BDTv3.mobSeqPop7z    ")/342-(%%'+..*$#).11-&'9Nct   |vneZQHA:1%    '1;EP]k{@e6MVN8lL/,DU\[ULD=3$zcPB:67<DO]mu`J4!$Cf5Wq~zeCgE#rbXSSUVUQMHECDEGHIHGFDDDDC@<71-+,18AIPW_hszgSA0! 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-$/N:/,-ZNC*'"V2O9+2>12TZa'9+&>7F2!1EIOdKR&:;)<(7QW2<(;- 40;), !'420*&29+IY  D>< E40^"F2&<(2c09}Wk=?h: h=)OD[7;1Y5#0Kx|5FZ Gz',{A< 52  V%O]w&|( jM_|c|M %&%Ce%lLV9.H5)t5-{q_\>sIF1m>)fdm Z$=)|I@F&}:qz"+bfL7yH#?,:wl~]h04IG`'']cIx[5=WA _3,=0z,0" 0Y{KHKlo 4s%>zLayE R0Wkx zykbad:m8E1,f=xrSzNlNB c)-k=$km tPn;*g?> 0T1Dz4$pybH,(V O7L2fC1v`pv HJA}^io #~%oc[w^Sa9n:=a4KSMA-)GL:0 &,;D68)WT,7%>.8>)%'#$!/$ !                                6 !  :  (.(!5%"%" * "5!)'=(7@1&-")24C(0/3D<,E5956:8GH.+)>RQQQ/DH7O3QVVULKN@]F<1TSRUKjBN[^mVb3ZhhRlllF\\S]QS__ku\m]\bRZooartJTne}hpuZv|h}!lcQ[lct?Ed!G.d X 1J 9kpphX|,RQ`76P"Co#IHU(o8lskpqc&,O BfGpR?-t HdD6p+{RXwP\HtilO3PdSdM!dba>#*!{o9;KdM*Sf+}TU{8dN@Q\G}4dwh':q pE|Z~ S|d"[,'vGw Sjpp,uVw$dp',Q,dpppppmy4U'8^qH}p<1Qr|X5<c)>=+C.6i5nS1|8xUE\,36$@A1xN^1*4-/-N/Oe!<%<$Q#V.$>N!G<0J';$,-8"V:8.==0,0,7/F+9;>#YS %*p3*G.<?5*<!:%,-'M+'.%;CP0E*?C/3L9G](@9H5)CnHc 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D&ng=L}07b}Tjm]GCGGCTTCTTAGTATACAGATTCGAAATATGTACATGTCGCATAAGATGTAATGCTACATAAGGGATGGGCTACGGCACCCTTTAAAGACGATTTAATAACCCCCCCTCCTCGCAAAACCCCCCGGAAAGCACATTTCTTCATGAGCAACTACTGGCGGGACTTTTACAGGGAACCTACTTTTTAATTTTTTGATGGCGCACCCAGGTTTCTACTCCTGCATTTCCTGGGAATTATGAGGGCTGTCTTGGATAGACAGCAAATTCGGTTTGTATTTCCCTGTGTACCAGAAATTGTATCGAGAATGTGGGATAAAAATAATACCGACGCGGAATGTGCGGTGTCCATTTAAGTGGAAGAGCAAGACATCCCTGTGTTATGCGACTAGTCACTCCGCGACTATAATATGGAGGGGCATAAACGCGGCGACTTCTACACCACTTTTTTTAGATAATAGACGAGAGAAGTACTGCGTTTAGCTGGGCCAGAAATAGACGTAGGACGCATGCGTGCTTTAGCAGAGTTATGGGCAAACAATACTGTCTAATACTACTCCGCATAATCATGGTCACATCTTTGTTAAGCTACGCATGCTGCTGTATATATCAGCGTGGCGTAGTAGACTCGCGTATTGACTCTCATCAAGACCGGTGTTATCTCGTGTGTGGATGTCAAGTTAACACTCGAGGATATAATGTGTAGACTTGAGGCBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 16:57:25 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtTASPtTB1PtSB1PtSBCTSLBufT JCMNT 0vCTIDJoCTNMJuCTOwJCTTL 0mCpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 'DATA %DATA 88ppTDATA 88pp DATA 88pp5}DATA 88ppDCHTDSamDySN 0NDye#DyeN0YDyeN0^DyeN0cDyeN0hDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0-HCFGCEHCFGHCFGHCFG>>InScInVtLANE\LAST~~KJLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISeP1AM2TP1RL,P1WD7P2AMBP2BAHP2RL=lPBAS^PBASPCON*PCON'<PDMFT}PDMF|PLOC!PLOC$PSZE`PTYPJPXLBRGNmJgRMXV1RMdNB;RMdV1RMdXBKRPrNJ-RPrV1RUND  RUND  RUND  RUND  RUNT 1RUNT 7 RUNT +RUNT 7Rate 0!RunN**J=S/N%]SCAN@SMED SMLtSMPLThSPACAkH`SPACuSPACAkH`SVER4.0SVER LSVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCHphDYbigphQLcphTRphTR#This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtTASPtTB1PtSB1PtSBCTSLBufT JCMNT 0vCTIDJoCTNMJuCTOwJCTTL 0mCpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 'DATA %DATA 88ppTDATA 88pp DATA 88pp5}DATA 88ppDCHTDSamDySN 0NDye#DyeN0YDyeN0^DyeN0cDyeN0hDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab[.FVoc[FWO_GATCFeat//ZGTyp!!0-HCFGCEHCFGHCFGHCFG>>InScInVtLANE\LAST~~KJLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISeP1AM2TP1RL,P1WD7P2AMBP2BAHP2RL=lPBAS^PBASPCON*PCON'<PDMFT}PDMF|PLOC!PLOC$PSZE`PTYPJPXLBRGNmJgRMXV1RMdNB;RMdV1RMdXBKRPrNJ-RPrV1RUND  RUND  RUND  RUND  RUNT 1RUNT 7 RUNT +RUNT 7Rate 0!RunN**J=S/N%]SCAN@SMED SMLtSMPLThSPACAkH`SPACuSPACAkH`SVER4.0SVER LSVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCHphDYbigphQLcphTRphTRMKdkij7"URQ`)e3T[.3Q~/]nLa0#C"4kyESilV'~\F>9HbM|(%: h4qKl}M2":Da^?vftu3ky+a/hV6XD9=6W\!3[s?z5O`Sdk-~4Kb}wLKkD[&C#]-@f9kf'zbO\8b790-I;dfAY(y'tMz/yxf/5f0]/joM`)6-_X.> %9WK] O7e<>/D?*&qDp-o+9mW'cj!Q4sIRD8z{q>t[XAn@ggWK!-D-g.wMwH9,K>GHKz!{bmKB_,qz7A*k|_Mt0mP`k(U.*)./&0@ejUZo-vNIGQ5EYLYswo,n`!3lCIezQd)l'[)VBT0?OC5wz^`Akc PkWhIdp"iK]jLH#[,TcM#yKAI8rBAlI{_*.9nWFa-st2hSXdF#lQ%cy-*7y 4/a"Xuj3~MEZv{:LByBI+Zw>5Alwe*vddD\4A,h{|dsgbk1^/.S3xCmqL}4|Md^_w&;H26.?bFW?E)X~6"den?,}&8)TK7\4v3s'kY6C}B-<`}l#[GVsI.J:*yEwiOfFD\4**+^cfoQek>HO7T3|2f[F6.9bEd9gP`koGhnWe^kQtAqjM>P\;Er|Dnm$7uM2^7f!]47.<2*VSU(]y"/:Boz?5wz^`Z#XTE-yh;}S$OKOfx&iJmW+E+n[yrA]#7w"OMbI.*R@_ajF=j&3d}7&I]N@c'v+gK >xPo\W'KFkaqQltX6jJ}Bq#pJE8m+adJs_-ZZ^4n\809b#.IglheVDR.No;nL#`5E^W6t{_"#k6l2WePm6W.#oU4v_DwW>qO9`}!&RW8X&tG98PbC.Ju5%2-8~$I]OuY6P,)[>M=suDfLs6)\R5y=Y`g%gcmea_';_/e"^=0UA!%EeKOWQ`QV-@[3>>B)u:[owQC(vJnPE*F;2_/gYFi$t_SF`|zvn=Y`1&!AuJUo6;^F6@_=!)}W}oo-o;X^`>EY$y!TW3nG6`VX+*fl{iG>A.8X:B?r@i$!^h{we**,ACT;fQGE-`x&i)6JWT|*0_fs$X=<;#(+gbk1^>7(FTcSO[mOEW<#GnP Qc$@)k,g5?wAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtTASPtTAUDTxxiAB1PtSB1PtSBCTSLBufT JCMNT 0vCTIDJoCTNMJuCTOwJCTTL 0mCpEPDATA@DATA@DATA@DATA@DATA DATA _DATA 'DATA %DATA 88ppTDATA 88pp DATA 88pp5}DATA 88ppDCHTDSamDySN 0NDye#DyeN0YDyeN0^DyeN0cDyeN0hDyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTab[.FVoc[FWO_GATCFeat//ZGTyp!!0-HCFGCEHCFGHCFGHCFG>>InScInVtLANE\LAST~~KJLIMS!!0LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISeP1AM2TP1RL,P1WD7P2AMBP2BAHP2RL=lPBAS^PBASPCON*PCON'<PDMFT}PDMF|PLOC!PLOC$PSZE`PTYPJPXLBRGNmJgRMXV1RMdNB;RMdV1RMdXBKRPrNJ-RPrV1RUND  RUND  RUND  RUND  RUNT 1RUNT 7 RUNT +RUNT 7Rate 0!RunN**J=S/N%]SCAN@SMED SMLtSMPLThSPACAkH`SPACuSPACAkH`SVER4.0SVER LSVER,,/Scal@Scan@TUBEC12Tmpr<UserphARphCHphDYbigphQLcphTRphTR