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"& "! !       +" ,         2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: ee5b5a9e9a6e4f23b74c25799743d412Seq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_C11_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z%-=V{?e 'Lw &/=N`mtupkfb^VH4v]I<4/+$"4H\o~kXD.~eN8# }gUE4~tkc]YVUTUVXZZZXTOJEBAEKS[`ccdhq 7iU&$`!j5cG0            "$%$$%(-2676421247;BM`|P.BA*AtVA2+-;Z]wC 8$8y]I( !%&%#!   !! <p~@lh5\SM%     =s[)AF# #>g851 j f2         >*B>lg"    9Z0im;b~4     "(,+&$G[*XP6o7!*/+ &z>b_o?[*7         !)0442-'""(+%u,1dfa:bq5$%z      +h)<n*      )1674-# (-.)! 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Y>505A''54**LWK + T,0%N`H G d]A+P?04<;54B&3L=01@E<[>&>**a?I 2,YI%^>%>!* ["CVGCI-MQ@NEAbDW2PG%]HWJX9B,NTO J&&XSc"M,H>\4c_1Z[$(2]#1$^YT#"B3DAM69;!EOT<Fb =_:4 OG 1Q4R!(S^21IK)%ZE*"a9T^!6S) _YVP OR0KGK1HE5# ?2%.]`'<)1>4%6Q?"J:& !]\;P)2&& F5'o\h5c,ssmEhm^R#G,LM[L|1Wneu;b"W4-( j [:iJx"L'OZ)E 9G2ryd1@zsD =+~Dh psMYW Z#=zRdv_-K ij^F va1 i+@jIUGoy;XkME4 ]Ss T5 j(;B`)d-=x] j|:d y_\WOD|@AWp( H Fh[7@}Y eZt T Z,H)0mBic ^\8> 8~V7`3@H%VU]   o vRe k;YpR:DzWMf'X%CZ3|K4}3(1y+(c]] a-3 H]DHV_yA+khloZDF^>_m)Y(R4/?/57+"& *)' (          #  *)*((" &% %*(8'%'50'!7/5+&$Q-'3/4)2+3/$%2408>94;AEB9B6P0;F?&F6:5*ZMNN@HCD@<PP8>E>VZjHZVWL\ZKnZUXOSNp_YWUfi]^ZZ`wu^ss^`ouvvvZwZyuxlx]tisskpppwss}xkkkssspptmrqstn8 ` `+ggggkgspsk|f`.pf4jppwkGrp^H4S2M6r` 1  Mt } zCbbQ K !tdC;oL3$ ,Hi2'8dS 0I#q=BI;Fucz/ EK1([:8kmO#tdQFlZud$p5_?ljb[g45`-Wj]2u,} 8lpxI }j zpIdpVpp/pp,p p\%pG m %.64U(so%<A r_ 81)9R0,}( `q%&7"Hn` >R'wKO&9]N> .6-9`-4<F -'#' '-Pyu_LV!%CH .A!GO 6@J8E. (.!G)6.!-2YbE0+(I."+=;\(7 5$71A&=' "4#5!$&@"55!>&0:3FX*R!%!#)!$?!6! %8 $*H1(5""&7@;".6L0[K[9-4.G;3/9]WL+S*[);'0%,!C%LL3R( d$"6$.'74Z#1%@0D63>!,$,8#A&Cl'; 1#,*s;48)BCWX*!3Eb.*4-,O!0A1*5-&!0X7_@*(K9=^d !35(@8#1.m%79/*0.%U63R1!Q/},!.=3%'NY,"X:F=/YA8=)(D"90O\.Q*60=xE'ahl*9G`SHh28C<D=dBoZ{d?Teq8=p1B-wqgJ9MA*'!`URQO=LRhosvydDL^]$~Na0?_Zc||Ix}!5`^TJLGGGAGGGGGTATCCCCCCACACATTCATAGAGTAACCCCAACAACAAGGACACACACACGGACCTCTACAACGTTCCCCATAGAATACCCATCTTTGGGTTGAATTAGTCCACCTAAGGCCCGCAGGATTTTGTGGGTCCTTTCCGTCTCTCACTACTAGAAGGGGAGGAGCCGAAGTTATAATTTTGGGGTATTAGGCGCCAGAATTACTCATTTCTCCAAGGCGAAGGGCTGGTGGGTTTGGGAGCCTACCTACTTCCCTTACTGGAGAGGTGAGGTTTTGAGTAGCACACCTTCCTACACGGAGATGGAGAGAATAGGCTACATAGGCGTTATATGGTTGCACCCCTCGGCCAGGGGTTAAACTATCATAGCTTCTACTTAAATAGGGAGGAGGAGGGATGGGTCTATCAGTCAACCCAGTAGCCTATGAACGAGTGTTTTTTAAAGAGGATAGGGAGCCTCTTGCTCCTTCTGATGTTGCTCGGGATCGAGAACACTTACGAGGGGCGGCGACAGTATAGTTGTCTCGGTTGTAATATTAAGAAGAGTATTATGTGATTCTGAATTCTAGATCACGACCTCAATCTCTACCACAGGTTCAATACTCGTCAGGTGTAATGACATABCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:22:34 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtGASPtGB1PtFB1PtFBCTSd{BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;wTDATA ;wuDATA ;wDWDATA ;w9DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE[LAST~~bLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS4#P1AMtLP1RLtGP1WDtQP2AMt[P2BAtt`~P2RLtVPBAStt6PBAStt4VPCONttEPCONttCPDMFTsPDMF4:PLOCt>&PLOCt9>PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%4SCAN@SMED SMLtSMPLT^SPACAiSPAC43SPACAiSVER4.0SVER dpSVER,,/Scal@Scan@TUBEC11Tmpr<UserphARphCH4QphDYbigphQLcphTRphTRjThis is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtGASPtGB1PtFB1PtFBCTSd{BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;wTDATA ;wuDATA ;wDWDATA ;w9DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabrFVocrFWO_GATCFeat//rGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE[LAST~~bLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS4#P1AMtLP1RLtGP1WDtQP2AMt[P2BAtt`~P2RLtVPBAStt6PBAStt4VPCONttEPCONttCPDMFTsPDMF4:PLOCt>&PLOCt9>PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%4SCAN@SMED SMLtSMPLT^SPACAiSPAC43SPACAiSVER4.0SVER dpSVER,,/Scal@Scan@TUBEC11Tmpr<UserphARphCH4QphDYbigphQLcphTRphTRTFo@L15FT!sz^2h3)-I561hB_f*>0-D%~{a,vT`S'MS{xCM&mUYa?Z?Rq}n{if?K;@WJG7jVYmW=GNx_|$xzW,DK >M7NJrj(oJQi)-PA)yw(~?V'i~o41{mXt@krT::_o5qSF1F7T3|2f[F6,6ir%u_bO?NL? nwt!}q|nc{[*Ml$.9@_05a=gHFhwD~ ')&o"J'8O[,?a9'Kdb"U"=C!(3b^ ppjWqke~HIW.|$-?vbN|U`K'H:{GKi1SkJKi|!%MQJ1r]C$p~/Ykf4;N^YEu'+=3yHt.&T, /Y+!mfbhP}7}_'AIh_`V$];9'k*|QK:}$OEH3SP')3g;Z6ObG`<(:&I;u4,ZE_GY@ZWeoa$sp@OJ-omMOx8yT,IlY'_2Acr]t>OE2(/(W,XD3J6EX! w+[_3sh(6(1[!O$j=%anSA.us]4@3)Gb2T|+adJs _ZZ^k42g|5(T3#T]TcS[7}?e|Eg0gv`k:!.tk.2=s]jh,cW`$~~hY8|k~_D}<|hG6`VsI{o7QCUTAk|k.j,PCH-p@vadJLYcF3E;qTg?USx&#v3"qo,n[4|M,S]ZD>tSEH$*YYo%+0|>w>6?xHf.QR9]CR"lWW .C&q6X+9muy%lFLuLot\zEUbW{/=~$9Zb{aGd7hVo+&k=;A{%i|o(>V&=o>Z$~y;|>,m_noM$mOYePWz"$QAjat>9.b':8 v=iqNs)lktayr=u46_n&)-%Tw1(_CcSb;6AI8rBAr&j6|MR_le/M#yKAI8rBAr_s_bed{a9_ajF=W.X}=_;!;`sEYWC.%ouO{9X6!7&o.jaxJK[Yl)d762H#A#Q9U,7Yv^TTH455-z]G3o6RlSR#yK9YtIRPV]O_,A8 z9X=5>2jP@*F8a?G>Sx+C!K}Z'BNpbX\"B=8djF3u2yl:[>zw#NRd)B*OqPk&OE6OLxPo%?E7\lzqdY(xs/b@j/.3]Hq<6(m=':Z^u4[42g|5PxKoJ-[CW?R5Ch2d'jw~|>J3zi^{g5AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtGASPtGAUDTxxB1PtFB1PtFBCTSd{BufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ;wTDATA ;wuDATA ;wDWDATA ;w9DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabrFVocrFWO_GATCFeat//rGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANE[LAST~~bLIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOIS4#P1AMtLP1RLtGP1WDtQP2AMt[P2BAtt`~P2RLtVPBAStt6PBAStt4VPCONttEPCONttCPDMFTsPDMF4:PLOCt>&PLOCt9>PSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%4SCAN@SMED SMLtSMPLT^SPACAiSPAC43SPACAiSVER4.0SVER dpSVER,,/Scal@Scan@TUBEC11Tmpr<UserphARphCH4QphDYbigphQLcphTRphTR