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"$"!  $#0)*'#,$  '$  (# ( ")" $"- " % !% 7.$ *)%( !#" !!*&*#""'7/1,-"&$&$(&) #*+: %(;   '5 )7%*'..!+%! &4,,)"- #* " '"-#  -'# /(# !5   2,,,III^^^{{{&&&99DDDNXXaaavvv      ***3>3GGGRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRRR =KPQRRQQQQPONMLKJIHFEDCB@>><;986532 KKKCCS_^rtt   "%%,--444;;;BBBIIIOOOVVV\\\bbbhhgnnmsssyxx}}}<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;<<;;;<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<+6258050-03 6258002-03 6258003-02 6258005-00 Run Started Run StoppedCollection StartedCollection Stopped) POP7 Z-BigDyeV3Dye1Dye2Dye3Dye4Comment: bc1115a6e0d74ff2b1ced21ab3c3c15cSeq50Pop7Z_5000SeqPop7z KB.bcpKB_3730_POP7_BDTv3.mobfalsefalsefalsefalse10.00KB_3730_POP7_BDTv3.mobQUJJRgBldGRpcgAAAAED/wAcAAAACAAAAdwAAACXAAAAAP////////////////////////////// //////////////////////////////////////////////////////////////////////////// //////////////////8WS0JfMzczMF9QT1A3X0JEVHYzLmJjY0NNTlQAAAABABIAAQAAAAEAAAAB AAAAAAAAAABDTkFNAAAAAQASAAEAAAAXAAAAFwAAAIAAAAAARFlFXwAAAAEABAACAAAAAQAAAAIA AwAAAAAAAEZXT18AAAABAAIAAQAAAAQAAAAER0FUQwAAAABTSEZDAAAAAQAEAAIAAAABAAAAAgAA AAAAAAAAU0hGRAAAAAEABAACAAAAAQAAAAIAAAAAAAAAAFNIRk4AAAABAAQAAgAAAAEAAAACAAAA AAAAAABTSEZTAAAAAQAEAAIAAAABAAAAAgAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 00.000falsefalse20false510false800false25.0falsetruefalsefalse20true5502042020420falseKB.bcp# 5Prime Basecaller Paramters Seq50Pop7Z_5000 Seq50Pop7Z_50001LongSeq50_POP71DC_Cap96_Fill17800DC_Cap96_EPS_ARC_SELF10DC_Prep_Time600DC_Scale_Divisor2.0DC_Down_Sample1DC_Laser_Power_Setting25.0DC_RS_Binning_PatternDC_RS_NumCapsDC_RS_CSDataOven_Temperature60PreRun_Voltage15.0DC_PreRun_Time180Injection_Voltage1.5DC_Injection_Time15First_ReadOut_Time300Second_ReadOut_Time300Exposure_Time0Run_Voltage8.5Voltage_Number_Of_Steps40Voltage_Step_Interval15Voltage_Tolerance0.6Current_Stability30.0DC_Ramp_Delay600DC_Data_Delay405DC_Run_Time5000Seq50Pop7Z_5000Run_ABI3730-82MY6V1_2015-10-30_11-34_18883130Seq15260131437_sgoyette_131437_654Rmfagan96-WellK1344_A10_ESRBCL654RKB_3730_POP7_BDTv3.mobSeqPop7z *2=JZjx/<EIHEA??BHOW`gotxxtk_QC9458;;6+ vqonlhaZTQQSUTOH?7200/-(!2DWlQT4nA(Kf}d9X%z{.Wr|eI,{>g ZgH-I~(Rnyt`@yH{slgdcba^YRKEBACGKORRPLF@<9751-)''*.24543332.(!#')(''()+*'!  #.7<=<96568;==<:877778:=BHLLG?5+%#%(+,,.7O{$8pNvei9q< "#!%-59;;;>BFJKLLMQW]`^YPG=60-++**(%!!$&'&%##%')*)+4KxpZvZ'  &/6;<91'$(+,,-..-+('+3AP^fhcZOC7,$"+Gxw89${? 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=+G8OJ:I4=O8KO2VQ\46+=0DF#Jb2W,8>%GQ,W!\8((Z:7T "F=`N(HCU?<` PUC!I2PVY6L1( L]U oce{+sMJ V8zXO@@3[)2@T  cxq$v.Xs  ?!b~_"rC8Aack0w8EC8h %)3R.?7=UH3,Lh-ZKl2rSZO9aAd.Q;v@Vkj uVK3^PAOe,RaJ[#,%e5iY{24 /4^F}<qWTk 3@Fubcf^vFb":dLFJ4n!c)k,B|nr;^KupvWH\_4>>@, hnA$BV a|3\Gs`YSTn6PO% !R^ \YMX/W^U 6VfJ1pt7T\`m\1{k  pSLQ7rF6q!$^@u%% > N|?xRcXDNeUUxXYay=5H2W_J8= %3-C"$$ )#/&> ":.!                     ) $$  .!, +&%)&N?6/*,$.-'21..&5@/05* ?>7?<@33;1DD.AU3E<B=39A7AF8CDDS5C9;HIYo@BIeVjeSUNL3W]m}xZJiYWX4]LU^aaaYia6UgxeNNNooodd`vwzuet|o}w~jpylv vfmooootTJ{Rp98;[wK8CP8 `t"wU/o,P6 ox?ED5] \O]dd+M ky{* FW*(4dgiS5K d]$|{$^23" 8+8xdpj"[+X|YM,VoIS'd#_q^]03H d:-b1LA8xKr:{~_.Zc" 78Ao8e~i \MdOdD:B*JdWRN?-,T xM[k:X :& ",d|-:p8ppp_p,0)]5[LN1Ec47@96W~|3!PJ>1~a,m,6BSN35[4+hLm_H|UqN=5!%#D =l)4(  !+K(GVEz>^h%1p]%=%2#49b0"1^#1K9&!7"'4K+= 6Ei(t?&*'J:*&7\C7 8",-b?IDoI<:F!)@*4 =,60>1 4.B!)")0+<;T!0B"(E,S <A)I?+"*$:Q F(&?%!@ B6f+2T$2FW0ZB_(A4+P*-8K%%35Uc#@ !/:$5";&5$+AP">-ED<a0*$2"%L;Z '(3"C>"5!$%2#6M4R_56&;;H 7 -3-@@(c(<De$a!%&S71+G+?U.1""<44k2/8#fVA&!,+A,$% &")+"I+O'B7G3_k$BOGL`T*%5B'9BVn\SF;=)O%)P7++1\j FA5/<t$&<?G5H& .8BA]H NQ<`Ycn+.LPb81]FIasl7%]Vt|\OEo/:WS2W!4%il|74|e=\H3 1GCCCAATTTAATTTTTGGAGGATACCAAACCCACCTGGGGTACGGAACCCGAACAACGTTGATTTCCACATCCGCCTCTACAACATCCCCCAAAAGAGGGCCTTATTAGGGCCTAGATCGCCCTCCAAACCCCCCAAGAAATTTGTGGGTCCATTTCCCCTTTCACACCTTGAGTCCTGTGTGCCGTTCAAATATTTTTCTAAGAGTACCCGCCAGAAATACGAGGCTCTCCAAGGCGTGGCCCGTGAAGAGTTAAATTTCAAGGCCCTTCCCTTACTGGGAATGTACCCGGTTGGAAGAGAAACCTTCCTAAAGGGGGCCCGAGGAAATAGGCTACTTCCGCTATCCCGTGTTCCCCTTCTAGAGCAAGGTTTTATATGCCAAACCATCGCCTTTGGAAGCGAAAAGGATTATAAACCTTTTCTGCAAACCGAGTCCCCAACTCCCTTGTATTTTTTTCGGAATATATTGAAGCTCGGCTTCCTACTGTGGTTGGGGGGCAAGGCGCCCCCACCAGAGGGGCTCCAACTTTATAACTATATCAATTTCGGTATAGCCCCCTGTATTATGAGACCGGCAATTATCAGACACAACTTAAGACTATACCACATGCTAAATACTCTGCGGGTGTATGACATBCPFileName=KB.bcp;AnalyzerVersion=KB 1.4.1.8;MobilityFileName=KB_3730_POP7_BDTv3.mob;StartPoint=0;BaseOneLocation=0;StopPoint=0;StopAtPCRStop=0;AverageSpacing=0;ProcessedDataProfile=0;StopAfterNumBases=0;MaxBasesToCall=800;CallMixedBases=0;SecondaryPeakThreshold=25;NCallQvThreshold=0;StopAfterNumNs=0;MaxNumNs=20;StopAfterNumNsInXBases=0;NumNsPerXBaseInterval=5;XBaseInterval=10; KB 1.4.1.82015-10-30 13:22:21 -04:00AEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTS~YBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?~*TDATA ?~*ҽDATA ?~*PDATA ?~*DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FWO_GATCGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANEPLAST~~|LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMCP1AM~fbP1RL~afP1WD~k^P2AM~uVP2BA~~zRP2RL~pZPBAS~~OPBAS~~MvPCON~~^PCON~~\jPDMFTsPDMFMZPLOC~WnPLOC~RrPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%M;SCAN@SMED SMLtSMPLT^SPACAf_SPACMSSPACAf_SVER4.0SVER ~NSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHMqphDYbigphQLcphTRphTR,}This is the confidence range *ABI_Limits..FVocAEPt@AEPt@APFN TAPXV2APrN 0APrV5APrX~~0ARTNASPFASPtASPtB1PtB1PtBCTS~YBufT JCMNT 0nCTIDJgCTNMJmCTOwJCTTL 0eCpEPDATA@DATA@DATA@DATA@DATA DATA ]DATA #DATA %DATA ?~*TDATA ?~*ҽDATA ?~*PDATA ?~*DCHTDSamDySN 0FDye#DyeN0QDyeN0VDyeN0[DyeN0`DyeWDyeW8DyeWSDyeWgEPVt!4EVNT /EVNT /EVNT/EVNT0FTabFVocFWO_GATCFeat//tGTyp!!0%HCFGCEHCFGHCFGHCFG>>InScInVtLANEPLAST~~|LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMCP1AM~fbP1RL~afP1WD~k^P2AM~uVP2BA~~zRP2RL~pZPBAS~~OPBAS~~MvPCON~~^PCON~~\jPDMFTsPDMFMZPLOC~WnPLOC~RrPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%M;SCAN@SMED SMLtSMPLT^SPACAf_SPACMSSPACAf_SVER4.0SVER ~NSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHMqphDYbigphQLcphTRphTR2I\(f^t8 e,&o.]/+M;`8>>~/H'JKo4<"}3C;PGYTe3"Q\|F{?5[3:im;S!?a9TghW~^N@.]neZ_kP)X^UM]aD&Wr`s;bqz^]/\4'\%E:"*lu`>U{mwYgE{}Vm)HNtXehkT/n@Z- O{C8T`T*#sKx'SF*$6elkgah(mae$*6I|t+7@7x,"L-2QvA- d+[Y.me|[{0%4dc{Ae`ox%CR"lyV?:w02"_pgr:vrG\boF3uXB23yKnu3B7UVxXm}j3q6A@ wvr]{;:Y2WHbxUM~*-6)H_v&'Y?sxWlwMXG9{k"K-Dty{J$XXt;J0FkeH=)Ak&Q\W{[yVh,p)wk]M[H#S_d.u?zdq3+fFc':Z^P?ZY{7+VDTj.uFgq%,NKo-,(B~bwj{}L]^k@_ajF=W.X}=";WjcHdkabCDkm[&HcXe"t}1%j-]Tsz^`A.u?s-u@G1fCy:*?GD8Y~Csk%pcyO_lh#m|!yrA]#7w"OMbq?Eb,Z#GlOjW^]=7l1%Em[.YYo%/D^*Pis|K/l3672'[)VBT9\qwH=qd)B*E\jE@r9#$%rnLjL3ro{`N%7`h?D,kSagv`k=U@xkk^CELhhSc%bVm? >S]*|QK:}$OEH3udrVbH`6@m rp%r3vEu`~#`V4{Zf&/7p9e R0]-jE(_y"DQX;:,292CjFnjUZ:U$n?XDD[ho*9wQi:A,fv7b{Wj2oFtqwGK"j+OC5wz^`Z]k7{d&]?#5ngDVD$3#2ps'Mvn[Ca,k?]mnjH[q?Eb,u2We:lFm%FHN] 5y$47y 4orKOR`{A,}@1%i`+|*A:B639u!k@U8`.pqLQUfu:_D\a$Fl@i2ps'Mvn}4|Md^_w&;H26t{_"#k<>Dj&['KD7I 95[Xv,Kho|[E,fcd]R03Wi8b9s|l}$rv/Z-\dXm4c1/`WajZUTw|]jl@i&RCSwToN6|1Dlag&<{[;N>InScInVtLANEPLAST~~|LIMS!!0{LNTD2LsrPaMCHNMODF0MODL3730NAVGNLNE`NOISMCP1AM~fbP1RL~afP1WD~k^P2AM~uVP2BA~~zRP2RL~pZPBAS~~OPBAS~~MvPCON~~^PCON~~\jPDMFTsPDMFMZPLOC~WnPLOC~RrPSZE`PTYPJPXLBRGNmJ_RMXV1RMdNB3RMdV1RMdXBCRPrNJ%RPrV1RUND  RUND  RUND  RUND  RUNT  "RUNT  RUNT  8;RUNT  Rate 0RunN**J5S/N%M;SCAN@SMED SMLtSMPLT^SPACAf_SPACMSSPACAf_SVER4.0SVER ~NSVER,,/Scal@Scan@TUBEA10Tmpr<UserphARphCHMqphDYbigphQLcphTRphTR